LOCUS       sequence-392753-        9424 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9424)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9424)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0974
FEATURES             Location/Qualifiers
     source          1..9424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2362..2950
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2415..2435)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2833..2844)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3022..3255
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3333..3454
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3370..3389)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3424..3443
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3494..3629
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3649..3668)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3650..3668)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3678..3695)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3678..3694)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3687..3709)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3836..4291
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3923..3942)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4133..4154
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4317..4421
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4422..4490,4491..5282)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4640..4659)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5453..6041
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5942..5961
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6195..6212
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6329..6350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6365..6370,6371..6388,6389..6395)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6403..6419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6408..6430
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6427..6443
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6427..6443
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6462..6482
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6464..6482
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6510..6736
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6737..6917
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6964..7089
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7582..7815
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8000..8044
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8275..8485
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8297..8493
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8344..8479
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8525..9127
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8525..8544)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9021..9041
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9170..9287
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgcc
     1201 acgctccttc ctggtgaaga gcaagaaggc tcacacctac caccagcccc gtgtgcagga
     1261 agatgaaccg ctctggcctc ctgcccttac cccggtgccc agagaccagg ctccaagcaa
     1321 cagccctgtc cttagcactc tattcccaaa ccagtgcctg gactggacca acctcaaacg
     1381 agagccggag ctggagcagg accagaactt ggccaggatg gccccggcac cagagggccc
     1441 cattgtgctg tcccgacccc aggatgggga ctctccactg tccgactcac ccccattcta
     1501 caagcctagc ttctcctggg acaccttggc cacaacctat ggccacagct accggcaggc
     1561 cccctccacc atgcagtcag ccttcctgga gcactccgtc agcctgtacg gcagtcctct
     1621 tgtgcccagc actgagcccg ccttggactt cagcctccgc tactccccag gcatggatgc
     1681 gtaccactgt gtgaagtgca acaaggtctt ctccacccct cacgggctcg aagtgcatgt
     1741 gcgacgctcc catagtggga cccggccctt cgcctgtgac atctgcggca aaaccttcgg
     1801 ccacgctgtg agcctggagc agcacacgca cgtccactcc caggggatcc cggccgggtc
     1861 cagtcctgag cctgcacctg accccccggg gcctcatttc ctccggcagg agcgcagctt
     1921 cgagtgccgc atgtgcggca aggccttcaa gcgctcgtcc acgctgtcca cccacctgct
     1981 catccactca gacacgcggc cctacccctg ccagttctgc ggcaagcgtt tccaccagaa
     2041 gtccgacatg aagaagcaca cctacatcca cacaggtgag aagccgcaca agtgccaggt
     2101 gtgcggaaag gccttcagcc agagctccaa cctcatcacc cacagccgca agcacacagg
     2161 cttcaagccc ttcagctgtg agctgtgcac caaaggcttc cagcgcaagg tggacctgcg
     2221 gcggcaccgc gagagccagc acaatctcaa gtagtaatga actagtccgt aacttgaaag
     2281 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaacagcagg gttgctctat
     2341 ggaccaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     2401 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     2461 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     2521 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     2581 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     2641 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     2701 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     2761 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     2821 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     2881 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     2941 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact
     3001 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     3061 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3121 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     3181 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     3241 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac
     3301 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     3361 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     3421 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     3481 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3541 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     3601 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     3661 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     3721 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     3781 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     3841 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     3901 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     3961 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4021 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4081 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4141 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4201 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4261 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     4321 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     4381 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4441 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     4501 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     4561 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     4621 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     4681 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4741 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4801 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4861 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4921 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4981 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5041 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5101 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5161 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     5221 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     5281 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     5341 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     5401 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5461 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5521 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5581 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5641 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5701 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5761 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5821 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5881 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5941 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6001 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6061 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6121 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6181 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     6241 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     6301 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     6361 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     6421 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     6481 gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg
     6541 caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg
     6601 ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct
     6661 gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct
     6721 agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     6781 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     6841 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     6901 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
     6961 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     7021 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     7081 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     7141 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     7201 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     7261 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     7321 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     7381 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     7441 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     7501 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     7561 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     7621 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     7681 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     7741 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     7801 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     7861 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     7921 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     7981 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     8041 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     8101 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     8161 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccat
     8221 gcattgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     8281 agaagtatgc aaagcatgcg tctcaattag tcagcaacca tagtcccgcc cctaactccg
     8341 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8401 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8461 ggaggctttt ttggaggcct aggcttttgc aaaaagcttt ctagaggtac caccggagct
     8521 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     8581 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     8641 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     8701 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc gtggcggtct ggaccacgcc
     8761 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     8821 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     8881 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     8941 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     9001 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     9061 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     9121 cagttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     9181 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     9241 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     9301 gggacagcag agatccactt tggcgccggc tcgaggcctg caggtgcaaa gatggataaa
     9361 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     9421 aatt
//
