LOCUS       sequence-392754-        8935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 8935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0698
FEATURES             Location/Qualifiers
     source          1..8935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1873..2461
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1926..1946)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2344..2355)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2533..2766
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2844..2965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2881..2900)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2935..2954
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3005..3140
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3160..3179)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3161..3179)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3189..3206)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3189..3205)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3198..3220)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3347..3802
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3434..3453)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3644..3665
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3828..3932
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3933..4001,4002..4793)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4151..4170)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4964..5552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5453..5472
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5706..5723
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5840..5861
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5876..5881,5882..5899,5900..5906)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5914..5930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5919..5941
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5938..5954
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5938..5954
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5973..5993
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5975..5993
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6021..6247
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6248..6428
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6475..6600
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7093..7326
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7511..7555
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7786..7996
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7808..8004
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7855..7990
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8036..8638
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8036..8055)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8532..8552
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8681..8798
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 ttgtgcctcc tgctcagctc cagggctcct gggctgctgg gaccaggctg aggggctcct
     1261 gggccctggc ccacagggac aacatggctg ccgggagcag ctggagctgt tccagacccc
     1321 gggttcctgt tacccagctc agccgctctc tccaggtcca cagcctcatg attctcccag
     1381 cctcctccag tgccccctgc cctcactgtc ccttggcccc gctgtggttg ctgaacctcc
     1441 tgtccagctg tcccccagcc ctctcctgtt tgcctcgcac actggttcca gcctgcaggg
     1501 gtcttcctct aagctcagtg ccctccagcc cagcctcacg gcccaaactg cccctccaca
     1561 gcccctcgag ctggagcatc ccaccagagg gaagctgggg tcctctcccg acaacccttc
     1621 ctctgccctg gggcttgcac gtctgcagag cagggagcac aaacctgctc tctcagcagc
     1681 cacttggcaa gggctggttg tggatcccag ccctcaccct ctcctggcct ttcctctgct
     1741 ctcctctgct caagtccact tctagtaatg aactagtccg taacttgaaa gtatttcgat
     1801 ttcttggctt tatatatctt gtggaaagga cgaggaagtc ggcctgtgga cttgtctacg
     1861 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     1921 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     1981 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     2041 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     2101 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     2161 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     2221 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     2281 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     2341 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     2401 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     2461 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     2521 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     2581 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     2641 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     2701 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     2761 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     2821 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     2881 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2941 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     3001 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3061 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3121 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     3181 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     3241 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     3301 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     3361 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3421 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3481 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     3541 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     3601 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     3661 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     3721 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     3781 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     3841 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3901 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3961 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4021 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4081 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4141 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4201 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4261 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4321 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4381 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4441 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4501 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4561 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4621 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4681 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4741 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4801 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     4861 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     4921 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4981 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5041 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5101 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5161 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5221 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5281 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5341 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5401 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5461 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5521 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5581 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5641 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     5701 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     5761 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     5821 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     5881 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     5941 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     6001 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     6061 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     6121 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     6181 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     6241 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6301 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6361 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6421 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     6481 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     6541 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     6601 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     6661 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     6721 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     6781 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     6841 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     6901 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     6961 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     7021 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     7081 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     7141 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     7201 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     7261 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     7321 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     7381 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     7441 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     7501 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     7561 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     7621 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     7681 atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat
     7741 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7801 caaagcatgc gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7861 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7921 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7981 tttggaggcc taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac
     8041 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     8101 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     8161 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     8221 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
     8281 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     8341 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     8401 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     8461 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     8521 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     8581 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg
     8641 aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     8701 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     8761 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     8821 gagatccact ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac
     8881 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaatt
//
