LOCUS       sequence-392755-        9409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9409)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0756
FEATURES             Location/Qualifiers
     source          1..9409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2347..2935
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2400..2420)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2818..2829)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3007..3240
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3318..3439
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3355..3374)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3409..3428
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3479..3614
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3634..3653)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3635..3653)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3663..3680)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3663..3679)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3672..3694)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3821..4276
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3908..3927)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4118..4139
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4302..4406
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4407..4475,4476..5267)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4625..4644)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5438..6026
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5927..5946
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6180..6197
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6314..6335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6350..6355,6356..6373,6374..6380)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6388..6404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6393..6415
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6412..6428
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6412..6428
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6447..6467
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6449..6467
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6495..6721
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6722..6902
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6949..7074
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7567..7800
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7985..8029
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8260..8470
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8282..8478
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8329..8464
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8510..9112
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8510..8529)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9006..9026
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9155..9272
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 aacacccttc tacggcgatg aggcgctgag cggcctgggc ggcggcgcca gtggcagcgg
     1261 cggcagcttc gcgtccccgg gccgcttgtt ccccggggcg cccccgacgg ccgcggccgg
     1321 cagcatgatg aagaaggacg cgctgacgct gagcctgagt gagcaggtgg cggcagcgct
     1381 caagcctgcg gccgcgccgc ctcctacccc cctgcgcgcc gacggcgccc ccagcgcggc
     1441 accccccgac ggcctgctcg cctctcccga cctggggctg ctgaagctgg cctcccccga
     1501 gctcgagcgc ctcatcatcc agtccaacgg gctggtcacc accacgccga cgagctcaca
     1561 gttcctctac cccaaggtgg cggccagcga ggagcaggag ttcgccgagg gcttcgtcaa
     1621 ggccctggag gatttacaca agcagaacca gctcggcgcg ggcgcggccg ctgccgccgc
     1681 cgccgccgcc gccggggggc cctcgggcac ggccacgggc tccgcgcccc ccggcgagct
     1741 ggccccggcg gcggccgcgc ccgaagcgcc tgtctacgcg aacctgagca gctacgcggg
     1801 cggcgccggg ggcgcggggg gcgccgcgac ggtcgccttc gctgccgaac ctgtgccctt
     1861 cccgccgccg ccacccccag gcgcgttggg gccgccgcgc ctggctgcgc tcaaggacga
     1921 gccacagacg gtgcccgacg tgccgagctt cggcgagagc ccgccgttgt cgcccatcga
     1981 catggacacg caggagcgca tcaaggcgga gcgcaagcgg ctgcgcaacc gcatcgccgc
     2041 ctccaagtgc cgcaagcgca agctggagcg catctcgcgc ctggaagaga aagtgaagac
     2101 cctcaagagt cagaacacgg agctggcgtc cacggcgagc ctgctgcgcg agcaggtggc
     2161 gcagctcaag cagaaagtcc tcagccacgt caacagcggc tgccagctgc tgccccagca
     2221 ccaggtgccc gcgtactagt aatgaactag tccgtaactt gaaagtattt cgatttcttg
     2281 gctttatata tcttgtggaa aggacgagaa tgatgctcct tgtatcactt aacgcgttaa
     2341 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2401 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2461 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     2521 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     2581 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     2641 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2701 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     2761 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     2821 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     2881 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     2941 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3001 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     3061 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3121 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3181 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3241 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     3301 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3361 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3421 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     3481 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3541 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3601 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     3661 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     3721 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     3781 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     3841 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3901 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3961 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4021 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4081 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4141 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4201 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4261 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4321 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4381 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4441 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4501 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4561 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4621 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4681 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4741 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4801 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4861 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4921 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4981 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5041 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5101 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5161 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5221 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5281 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5341 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5401 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5461 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5521 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5581 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5641 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5701 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5761 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5821 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5881 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5941 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6001 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6061 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6121 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6181 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     6241 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     6301 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     6361 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     6421 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     6481 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     6541 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     6601 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     6661 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     6721 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6781 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6841 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6901 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     6961 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     7021 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     7081 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     7141 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     7201 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     7261 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     7321 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     7381 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     7441 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     7501 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     7561 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     7621 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     7681 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     7741 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     7801 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     7861 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     7921 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     7981 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     8041 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     8101 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     8161 accattatcg tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat
     8221 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8281 atgcgtctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     8341 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8401 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8461 ggcctaggct tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta
     8521 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     8581 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     8641 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     8701 gtgggtcgcg gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc
     8761 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     8821 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     8881 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     8941 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     9001 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     9061 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct
     9121 agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     9181 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     9241 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     9301 cactttggcg ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag
     9361 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
