LOCUS       sequence-392756-        9385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9385)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0789
FEATURES             Location/Qualifiers
     source          1..9385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2323..2911
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2376..2396)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2794..2805)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2983..3216
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3294..3415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3331..3350)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3385..3404
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3455..3590
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3610..3629)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3611..3629)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3639..3656)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3639..3655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3648..3670)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3797..4252
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3884..3903)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4094..4115
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4278..4382
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4383..4451,4452..5243)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4601..4620)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5414..6002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5903..5922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6156..6173
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6290..6311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6326..6331,6332..6349,6350..6356)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6364..6380
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6369..6391
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6388..6404
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6388..6404
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6423..6443
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6425..6443
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6471..6697
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6698..6878
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6925..7050
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7543..7776
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7961..8005
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8236..8446
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8258..8454
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8305..8440
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8486..9088
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8486..8505)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8982..9002
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9131..9248
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgct
     1201 ccccagcgcg gtcgcagccc acgccggtgc gtactgggac gttgtggctt cctccgcgct
     1261 cctcaacctc cccgctgcgc ccggctttgg caacctgggc aagagtttcc tgatcgagaa
     1321 tttgctgcgg gtcgggggcg ccccaacgcc caggctgcag ccgcccgcgc cccacgaccc
     1381 ggcgaccgcc ctggccaccg cgggcgcgca gctccggccc ctgcctgcta gcccagttcc
     1441 cctaaagctg tgccccgcag ccgaacaagt tagccccgcc ggggcgccct acggaacgcg
     1501 gtgggctttt caagtgctca gtccctctgc ggacagtgcg aggctgccgg gccgggctcc
     1561 gggggaccga gactgtacct tccagccttc agcgccagca ccttccaaac ctttccttct
     1621 gagcaccccg ccattctact cggcgtgctg cggtgggtcc tgtcggcgcc ctgcatcctc
     1681 cactgctttt ccaagagaag agagcatgct gcctctcctg acacaggact ctaattccaa
     1741 agctcggagg ggcattttaa gaagagctgt cttttctgag gaccagagaa aggctctgga
     1801 gaaaatgttt cagaaacaga aatatatcag caaaacagac cgaaagaaac ttgccatcaa
     1861 cttgggacta aaggaatcac aggtgaaaat ttggtttcag aacaggagga tgaaatggcg
     1921 gaattccaaa gaaaaggaag tgctttccaa caggtgtatc caagaagtag gtcttcaaga
     1981 ggatcccctc tcacggtctg ctctgggttt cccttctcca tgtccttcaa tatgggacgt
     2041 cccccaacag cactcaagtc caagatggag ggagaattct ccagaacctt cagaaagact
     2101 aatccaggag agttcagggg caccaccccc agaagcaaat tcactgcaag gtgccttata
     2161 tttatgttct gaagaagaag ctggaagcaa gggtgtactt actggggctg tctagtaatg
     2221 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     2281 cgagaacgcc gggaggtggt cggtcggacg cgttaagtcg acaatcaacc tctggattac
     2341 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     2401 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     2461 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     2521 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     2581 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     2641 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     2701 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     2761 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     2821 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     2881 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     2941 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     3001 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     3061 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     3121 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     3181 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     3241 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     3301 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3361 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     3421 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3481 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3541 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     3601 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     3661 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3721 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3781 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     3841 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     3901 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     3961 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4021 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4081 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4141 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4201 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     4261 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4321 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4381 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4441 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4501 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4561 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4621 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4681 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4741 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4801 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4861 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4921 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4981 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5041 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5101 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5161 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5221 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5281 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5341 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5401 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5461 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5521 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5581 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5641 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5701 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5761 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5821 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5881 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5941 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6001 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6061 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6121 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6181 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     6241 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     6301 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     6361 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     6421 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     6481 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     6541 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     6601 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     6661 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     6721 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6781 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6841 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     6901 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     6961 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     7021 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     7081 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     7141 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     7201 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     7261 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     7321 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     7381 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     7441 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     7501 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     7561 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     7621 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     7681 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     7741 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     7801 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     7861 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     7921 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     7981 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     8041 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     8101 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     8161 cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag
     8221 tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc
     8281 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8341 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     8401 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
     8461 tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     8521 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     8581 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     8641 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     8701 cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtattcg ccgagatcgg
     8761 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     8821 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     8881 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     8941 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     9001 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     9061 catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa atggcagtat
     9121 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     9181 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     9241 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggcct
     9301 gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
     9361 ctaggtcttg aaaggagtgg gaatt
//
