LOCUS       sequence-392775-        8928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human CD283 protein expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8928)
  TITLE     TREAT-AD Plasmids 2023
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCDNA3-hTLR3-VenusFlag
FEATURES             Location/Qualifiers
     source          1..8928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    911..935
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3651..3675)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3686..3709
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(3740..3742,3743..3745,3746..4459)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3785..3806)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4046..4065)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4393..4414
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4499..4518)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4543..4591
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4793..5221
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4880..4899)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5090..5111
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5230..5250)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5235..5564
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5415..5550
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5477..5496
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5631..6425
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5685..5704)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6295..6314
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6601..6722
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6638..6657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6692..6711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6771..6787)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6771..6787)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6784..6806)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6795..6811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6819..6825,6826..6843,6844..6849))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6864..6885
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7002..7019)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7173..7761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7253..7272)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7932..8723,8724..8792))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8555..8574
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8793..8897)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgctagt
      901 taagctagca acaagtttgt acaaaaaagt tggcaccatg agacagactt tgccttgtat
      961 ctacttttgg gggggccttt tgccctttgg gatgctgtgt gcatcctcca ccaccaagtg
     1021 cactgttagc catgaagttg ctgactgcag ccacctgaag ttgactcagg tacccgatga
     1081 tctacccaca aacataacag tgttgaacct tacccataat caactcagaa gattaccagc
     1141 cgccaacttc acaaggtata gccagctaac tagcttggat gtaggattta acaccatctc
     1201 aaaactggag ccagaattgt gccagaaact tcccatgtta aaagttttga acctccagca
     1261 caatgagcta tctcaacttt ctgataaaac ctttgccttc tgcacgaatt tgactgaact
     1321 ccatctcatg tccaactcaa tccagaaaat taaaaataat ccctttgtca agcagaagaa
     1381 tttaatcaca ttagatctgt ctcataatgg cttgtcatct acaaaattag gaactcaggt
     1441 tcagctggaa aatctccaag agcttctatt atcaaacaat aaaattcaag cgctaaaaag
     1501 tgaagaactg gatatctttg ccaattcatc tttaaaaaaa ttagagttgt catcgaatca
     1561 aattaaagag ttttctccag ggtgttttca cgcaattgga agattatttg gcctctttct
     1621 gaacaatgtc cagctgggtc ccagccttac agagaagcta tgtttggaat tagcaaacac
     1681 aagcattcgg aatctgtctc tgagtaacag ccagctgtcc accaccagca atacaacttt
     1741 cttgggacta aagtggacaa atctcactat gctcgatctt tcctacaaca acttaaatgt
     1801 ggttggtaac gattcctttg cttggcttcc acaactagaa tatttcttcc tagagtataa
     1861 taatatacag catttgtttt ctcactcttt gcacgggctt ttcaatgtga ggtacctgaa
     1921 tttgaaacgg tcttttacta aacaaagtat ttcccttgcc tcactcccca agattgatga
     1981 tttttctttt cagtggctaa aatgtttgga gcaccttaac atggaagata atgatattcc
     2041 aggcataaaa agcaatatgt tcacaggatt gataaacctg aaatacttaa gtctatccaa
     2101 ctcctttaca agtttgcgaa ctttgacaaa tgaaacattt gtatcacttg ctcattctcc
     2161 cttacacata ctcaacctaa ccaagaataa aatctcaaaa atagagagtg atgctttctc
     2221 ttggttgggc cacctagaag tacttgacct gggccttaat gaaattgggc aagaactcac
     2281 aggccaggaa tggagaggtc tagaaaatat tttcgaaatc tatctttcct acaacaagta
     2341 cctgcagctg actaggaact cctttgcctt ggtcccaagc cttcaacgac tgatgctccg
     2401 aagggtggcc cttaaaaatg tggatagctc tccttcacca ttccagcctc ttcgtaactt
     2461 gaccattctg gatctaagca acaacaacat agccaacata aatgatgaca tgttggaggg
     2521 tcttgagaaa ctagaaattc tcgatttgca gcataacaac ttagcacggc tctggaaaca
     2581 cgcaaaccct ggtggtccca tttatttcct aaagggtctg tctcacctcc acatccttaa
     2641 cttggagtcc aacggctttg acgagatccc agttgaggtc ttcaaggatt tatttgaact
     2701 aaagatcatc gatttaggat tgaataattt aaacacactt ccagcatctg tctttaataa
     2761 tcaggtgtct ctaaagtcat tgaaccttca gaagaatctc ataacatccg ttgagaagaa
     2821 ggttttcggg ccagctttca ggaacctgac tgagttagat atgcgcttta atccctttga
     2881 ttgcacgtgt gaaagtattg cctggtttgt taattggatt aacgagaccc ataccaacat
     2941 ccctgagctg tcaagccact acctttgcaa cactccacct cactatcatg ggttcccagt
     3001 gagacttttt gatacatcat cttgcaaaga cagtgccccc tttgaactct ttttcatgat
     3061 caataccagt atcctgttga tttttatctt tattgtactt ctcatccact ttgagggctg
     3121 gaggatatct ttttattgga atgtttcagt acatcgagtt cttggtttca aagaaataga
     3181 cagacagaca gaacagtttg aatatgcagc atatataatt catgcctata aagataagga
     3241 ttgggtctgg gaacatttct cttcaatgga aaaggaagac caatctctca aattttgtct
     3301 ggaagaaagg gactttgagg cgggtgtttt tgaactagaa gcaattgtta acagcatcaa
     3361 aagaagcaga aaaattattt ttgttataac acaccatcta ttaaaagacc cattatgcaa
     3421 aagattcaag gtacatcatg cagttcaaca agctattgaa caaaatctgg attccattat
     3481 attggttttc cttgaggaga ttccagatta taaactgaac catgcactct gtttgcgaag
     3541 aggaatgttt aaatctcact gcatcttgaa ctggccagtt cagaaagaac ggataggtgc
     3601 ctttcgtcat aaattgcaag tagcacttgg atccaaaaac tctgtacatt tgccaacttt
     3661 cttgtacaaa gtggttgatc tcgaggacta caaggacgac gatgacaagg gagggggtgg
     3721 atctggaggg ggtggatcta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     3781 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     3841 cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct
     3901 gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg
     3961 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4021 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4081 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4141 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcac
     4201 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
     4261 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4321 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
     4381 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     4441 ggacgagctg tacaagtaag gtaccggtta gtaatgagtt taaacggggg aggctaactg
     4501 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     4561 ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     4621 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     4681 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     4741 gcaggccctg ccatagcaga tctgcgcagc tggggctcta gggggtatcc ccacgcgccc
     4801 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4861 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4921 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4981 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5041 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5101 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5161 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5221 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     5281 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     5341 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     5401 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     5461 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     5521 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     5581 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     5641 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     5701 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     5761 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     5821 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     5881 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5941 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     6001 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     6061 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     6121 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     6181 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     6241 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     6301 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     6361 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     6421 tctgagcggg actctggggt tcgcgaaatg accgaccaag cgacgcccaa cctgccatca
     6481 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     6541 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     6601 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6661 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6721 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     6781 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6841 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6901 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6961 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7021 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7081 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7141 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7201 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7261 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7321 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7381 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7441 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7501 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7561 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7621 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7681 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7741 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7801 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7861 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7921 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7981 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8041 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8101 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8161 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8221 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8281 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8341 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8401 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8461 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8521 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8581 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8641 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8701 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8761 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8821 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8881 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
