LOCUS       sequence-392778-        9100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector with tet-inducible porcine DUXC/human DUX4 chimera 
            (codon altered).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9100)
  AUTHORS   Nip Y, Bennett SR, Smith AA, Jones TI, Jones PL, Tapscott SJ
  TITLE     Human DUX4 and porcine DUXC activate similar early embryonic 
            programs in pig muscle cells: implications for preclinical models of
            FSHD.
  JOURNAL   Hum Mol Genet. 2023 Feb 2:ddad021. doi: 10.1093/hmg/ddad021.
  PUBMED    36728804
REFERENCE   2  (bases 1 to 9100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2023 Feb 2:ddad021. doi: 10.1093/hmg/ddad021."
COMMENT     Sequence Label: pCW57.1-pDUXChd:hDUX4ctd-ca
FEATURES             Location/Qualifiers
     source          1..9100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    71..188
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        242..556
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    250..268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    286..304
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    321..339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    357..375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    393..411
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    428..446
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    464..482
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     529..553
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      571..580
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1903..2027)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2045..2062
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2072..2582
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2501..2519
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2592..3188
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2592..2611)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3088..3108
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3189..3242
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3243..3860,3861..3866,3867..3902,3903..3905,3906..3941,
                     3942..3944,3945..3980,3981..3989)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4029..4049)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4447..4458)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4663..4896
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4968..5089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5005..5024)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5059..5078
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5129..5264
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5264..5335
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5336..5404,5405..6196)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5554..5573)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6367..6955
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6856..6875
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7109..7126
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7243..7264
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7279..7284,7285..7302,7303..7309)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7317..7333
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7322..7344
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7341..7357
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7341..7357
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7376..7396
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7378..7396
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7424..7650
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7651..7831
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7878..8003
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8496..8729
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8914..8958
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttcagaccca cctcccaacc ccgaggggac aattctcgac ctcgagacaa atggcagtat
       61 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
      121 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
      181 aaaattttcg ggtttattac agggacagca gagatccact ttggccgcga atcgatatgt
      241 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      301 gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc
      361 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
      421 gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
      481 gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt
      541 gaaccgtcag atcgcctgga gaattggcta gccaccatgg actctagctc tacatcttgt
      601 actagctctg gaagcctcag ccgcgggtct aggaggagac gcctggtgct caagcaaagc
      661 caacgcgacg ccctccaggc cgtgttccag gaaaaacctt atcccggcat caccacacgc
      721 gagcgcctcg ctcgcgagct gtccattccc gagtcccgca tccaagtctg gtttcagaat
      781 cagcgcaagc gccgcctgaa acaacaatcc cgcggccctc ccgaaaccat tcctcagccc
      841 ggcccccccc aacgcgaaca gcaactgcaa accagcccta cccccgccat tcccaaggaa
      901 gccggccgca aacgcacctt tattagccct agccagaccg atattctgcg ccaggctttc
      961 gaacgcgagc gctatcccgg gatcgctgct cgcgaggagc tcgcccgcca gaccggcatc
     1021 cccgagcccc aaatcctcgt ctggttccaa aatcgccgcg cccgccatcc cgaacaaaaa
     1081 ggcagcggcc ctgctcaggc cggtggactc tgctctgctg cccctggggg cggccatcca
     1141 gcaccttcct gggtggcttt cgctcatact ggcgcttggg gtaccgggct gcctgctccg
     1201 catgttccct gtgctccagg ggccctcccg cagggagcgt ttgtttccca ggcagctagg
     1261 gctgcacctg ccctgcaacc atcacaggca gcgccagctg aaggcatcag ccaacccgcc
     1321 ccagcccgcg gagattttgc ttatgcagcg ccagcacctc cagacggtgc cctgagccac
     1381 ccccaagccc ccagatggcc ccctcaccct ggtaagtccc gggaagaccg cgatccccaa
     1441 cgagatggac tgcccggtcc ttgcgctgtg gcccagccag gacctgctca agccggccct
     1501 caggggcaag gagtgctggc cccacctaca agccagggat ctccctggtg gggttgggga
     1561 cgcggacctc aggttgctgg agccgcttgg gagcctcagg ccggagctgc accgccgcca
     1621 caaccggccc ctcccgacgc gtcagcgtcc gcccgacaag gccagatgca gggaatccca
     1681 gcacctagcc aagctcttca agagcctgcc ccttggagcg cactgccgtg tgggctgctc
     1741 ctggatgaac tcctggccag cccagaattt ctccagcagg cacagccact cctggaaaca
     1801 gaagctccgg gagagctcga agcctccgaa gaagcagcaa gcctggaggc acctctttcc
     1861 gaggaggagt atagagccct tctggaagaa ctttgagtcg accatagtga ctggatatgt
     1921 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     1981 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttta gtaatgaacc
     2041 ggtccaccac caccaccacc actaaggatc cggggttggg gttgcgcctt ttccaaggca
     2101 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
     2161 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
     2221 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
     2281 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
     2341 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
     2401 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
     2461 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
     2521 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
     2581 agcaattcac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     2641 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     2701 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     2761 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     2821 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     2881 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     2941 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     3001 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     3061 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     3121 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     3181 ccggtgccga aggtagaggt tctctcctca cttgtggtga tgttgaagaa aaccctggtc
     3241 caatgtctag actggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg
     3301 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     3361 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     3421 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
     3481 ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     3541 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     3601 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     3661 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     3721 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
     3781 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     3841 agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc
     3901 tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg
     3961 attttgacct tgacatgctc cccgggtaac taagtaagga tcgatccaag atatcgtatt
     4021 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4081 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4141 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4201 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4261 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4321 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     4381 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4441 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4501 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4561 ccgcctgttt cgcctcggcg tccggactag aggtaccttt aagaccaatg acttacaagg
     4621 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4681 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     4741 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4801 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4861 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     4921 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     4981 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5041 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     5101 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5161 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     5221 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctttcaa atatgtatcc
     5281 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5341 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5401 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5461 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5521 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5581 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5641 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5701 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5761 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5821 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5881 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5941 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6001 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6061 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6121 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6181 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6241 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6301 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6361 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6421 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6481 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6541 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6601 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6661 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6721 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6781 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6841 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6901 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6961 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7021 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7081 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     7141 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     7201 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     7261 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     7321 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     7381 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     7441 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     7501 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     7561 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     7621 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     7681 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7741 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7801 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     7861 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     7921 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     7981 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8041 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8101 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     8161 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     8221 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8281 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     8341 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     8401 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     8461 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     8521 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     8581 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     8641 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     8701 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     8761 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     8821 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     8881 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     8941 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     9001 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     9061 atagtgaata gagttaggca gggatattca ccattatcgt
//
