LOCUS       sequence-392780-        8744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector with tet-inducible 3xFLAG-NLS-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8744)
  AUTHORS   Nip Y, Bennett SR, Smith AA, Jones TI, Jones PL, Tapscott SJ
  TITLE     Human DUX4 and porcine DUXC activate similar early embryonic 
            programs in pig muscle cells: implications for preclinical models of
            FSHD.
  JOURNAL   Hum Mol Genet. 2023 Feb 2:ddad021. doi: 10.1093/hmg/ddad021.
  PUBMED    36728804
REFERENCE   2  (bases 1 to 8744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2023 Feb 2:ddad021. doi: 10.1093/hmg/ddad021."
COMMENT     Sequence Label: pCW57.1-3xFLAG-NLS-GFP
FEATURES             Location/Qualifiers
     source          1..8744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     468..487
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     721..738
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    855..876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(891..896,897..914,915..921)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    929..945
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     934..956
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     953..969
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     953..969
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     988..1008
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        990..1008
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1036..1262
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1263..1443
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1490..1615
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2108..2341
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2526..2570
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2783..2900
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2954..3268
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2962..2980
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2998..3016
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3033..3051
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3069..3087
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3105..3123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3140..3158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3176..3194
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3241..3265
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3349..3358
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3358..3423
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3448..3468
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3469..3471,3472..4185)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3469..3471,3472..4182)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3511..3532)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3772..3791)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4119..4140
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4240..4256)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4241..4257)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    complement(4259..4383)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4401..4418
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4428..4938
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4857..4875
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4948..5544
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4948..4967)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5444..5464
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5545..5598
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5599..6216,6217..6222,6223..6258,6259..6261,6262..6297,
                     6298..6300,6301..6336,6337..6345)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6385..6405)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6803..6814)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7019..7252
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7324..7445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7361..7380)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7415..7434
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7485..7620
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7620..7691
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7692..7760,7761..8552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7910..7929)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8723..8744,1..567)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
       61 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      121 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
      181 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
      241 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
      301 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
      361 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      421 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      481 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      541 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      601 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      661 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
      721 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
      781 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
      841 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      901 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      961 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     1021 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     1081 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     1141 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     1201 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     1261 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     1321 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     1381 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     1441 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     1501 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     1561 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     1621 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     1681 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     1741 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     1801 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     1861 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     1921 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     1981 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     2041 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     2101 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     2161 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     2221 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     2281 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     2341 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     2401 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     2461 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     2521 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     2581 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     2641 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     2701 caccattatc gtttcagacc cacctcccaa ccccgagggg acaattctcg acctcgagac
     2761 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2821 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2881 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc
     2941 gaatcgatat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc
     3001 ctatcagtga tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc
     3061 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
     3121 agaacgtatg tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct
     3181 atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag
     3241 agctcgttta gtgaaccgtc agatcgcctg gagaattggc tagaactagt ggatcccccg
     3301 ggctgcaggg aatacaagct acttgttctt tttgcaggat ccctgcaggc caccatggac
     3361 tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga tgacgatgac
     3421 aagcccccca agaggatgag gagggagcca aaaaagaaga gaaaggtagt gagcaagggc
     3481 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3541 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3601 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3661 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3721 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3781 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3841 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3901 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     3961 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     4021 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     4081 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     4141 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgtcgac
     4201 caaggcctct cgagcctcta gaaccgatat caagcttatc gataccgtcg acctcgacca
     4261 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
     4321 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
     4381 ggtttagtaa tgaaccggtc caccaccacc accaccacta aggatccggg gttggggttg
     4441 cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc
     4501 gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc
     4561 acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta
     4621 agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt
     4681 ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga
     4741 tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg
     4801 gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt
     4861 tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc
     4921 accgacctct ctccccagca attcaccatg accgagtaca agcccacggt gcgcctcgcc
     4981 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     5041 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     5101 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     5161 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     5221 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     5281 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     5341 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     5401 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     5461 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     5521 tgcatgaccc gcaagcccgg tgccgaaggt agaggttctc tcctcacttg tggtgatgtt
     5581 gaagaaaacc ctggtccaat gtctagactg gacaagagca aagtcataaa cggagctctg
     5641 gaattactca atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg
     5701 ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc
     5761 ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca
     5821 tggcaagact ttctgcggaa caacgccaag tcataccgct gtgctctcct ctcacatcgc
     5881 gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa
     5941 aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg
     6001 tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca
     6061 aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt
     6121 gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata
     6181 tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat
     6241 tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct
     6301 gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaactaag taaggatcga
     6361 tccaagatat cgtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6421 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6481 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6541 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6601 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6661 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6721 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6781 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6841 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6901 cctttgggcc gcctccccgc ctgtttcgcc tcggcgtccg gactagaggt acctttaaga
     6961 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     7021 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     7081 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7141 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7201 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     7261 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     7321 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7381 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7441 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     7501 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7561 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7621 tttcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7681 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7741 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7801 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7861 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7921 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7981 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8041 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8101 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8161 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8221 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8281 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8341 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8401 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8461 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8521 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8581 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8641 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8701 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgc
//
