LOCUS       sequence-392782-        9184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivector with tet-inducible porcine DUXC (codon altered).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Nip Y, Bennett SR, Smith AA, Jones TI, Jones PL, Tapscott SJ
  TITLE     Human DUX4 and porcine DUXC activate similar early embryonic 
            programs in pig muscle cells: implications for preclinical models of
            FSHD.
  JOURNAL   Hum Mol Genet. 2023 Feb 2:ddad021. doi: 10.1093/hmg/ddad021.
  PUBMED    36728804
REFERENCE   2  (bases 1 to 9184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2023 Feb 2:ddad021. doi: 10.1093/hmg/ddad021."
COMMENT     Sequence Label: pCW57.1-pDUXCca
FEATURES             Location/Qualifiers
     source          1..9184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    47..164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        218..532
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    226..244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    262..280
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    297..315
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    333..351
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    369..387
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    404..422
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    440..458
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1963..2087)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2105..2122
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        2132..2642
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2561..2579
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2652..3248
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2652..2671)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3148..3168
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3249..3302
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3303..3920,3921..3926,3927..3962,3963..3965,3966..4001,
                     4002..4004,4005..4040,4041..4049)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(4089..4109)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4507..4518)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4723..4956
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5028..5149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5065..5084)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5119..5138
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5189..5324
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        5324..5395
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5396..5464,5465..6256)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5614..5633)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6427..7015
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6916..6935
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7169..7186
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7303..7324
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7339..7344,7345..7362,7363..7369)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7377..7393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7382..7404
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7401..7417
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7401..7417
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7436..7456
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7438..7456
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7484..7710
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7711..7891
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7938..8063
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8556..8789
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8974..9018
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggggacaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
       61 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      121 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      181 acagcagaga tccactttgg ccgcgaatcg atatgtcgag tttactccct atcagtgata
      241 gagaacgtat gtcgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
      301 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
      361 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
      421 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
      481 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagaat
      541 tggctagcca ccatggactc tagctctaca tcttgtacta gctctggaag cctcagccgc
      601 gggtctagga ggagacgcct ggtgctcaag caaagccaac gcgacgccct ccaggccgtg
      661 ttccaggaaa aaccttatcc cggcatcacc acacgcgagc gcctcgctcg cgagctgtcc
      721 attcccgagt cccgcatcca agtctggttt cagaatcagc gcaagcgccg cctgaaacaa
      781 caatcccgcg gccctcccga aaccattcct cagcccggcc ccccccaacg cgaacagcaa
      841 ctgcaaacca gccctacccc cgccattccc aaggaagccg gccgcaaacg cacctttatt
      901 agccctagcc agaccgatat tctgcgccag gctttcgaac gcgagcgcta tcccgggatc
      961 gctgctcgcg aggagctcgc ccgccagacc ggcatccccg agccccaaat cctcgtctgg
     1021 ttccaaaatc gccgcgcccg ccatcccgaa caaaaaggca gcggcagcgc taacgtccct
     1081 ggcgtggatc ctaacagcgc caaggggctg cccctgccca gcgatcaagg gatgcccaca
     1141 accgctcatt cctcccccac ccatagcgcg ccacccccac caagcaatcc tcctcgcgaa
     1201 aatatgctca gcattacacc tatggtcgct accgccgcca ttgctcctaa gtttatcgtg
     1261 cccggcgccc ctaccgccgg gtgcgaaggg caatccctcc ctatgatttt tattatggct
     1321 caaccctccc ctgtgctcca agccatcgtc aatcccccta tgctgtggac cctgccactc
     1381 acccaaagca gccccggccc tatgcctatc cccgctggcg gactgacccc catccatacc
     1441 ggcctgtggc ctaccagcca ggagggcccc tggcaagaaa ataacctcca taccatgccc
     1501 gccgagaagt gtctgcctca tattccccaa cctccactgg catcccgcgc cgaacctctc
     1561 cccctcctcg atcccgtcaa aacatgtacc tacgctcgcc ctgagtgggc tcaagccagc
     1621 agcgcccagg tgaccagcgg caaacccgtc cacggcgcta tgctccaacc cgcccaagcc
     1681 gataccctga tttgtcctcc ccacctcgct cctagcaacg aggaactctg tccccctatc
     1741 gatctccaac aaaataaacc tagcgctttt caagggagca gcaatctgct ggaagagatc
     1801 atggccgccg ccgggatcct ccccgaagcc ggccccctgc ccgatgtcga agagcaagag
     1861 gaactgcctc tgggcgatct cgaggcccct ctgagcgaag aggactttca agcacttttg
     1921 gatatgttgc cttccagccc tggcccatgc ccctaagtcg accatagtga ctggatatgt
     1981 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     2041 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttta gtaatgaacc
     2101 ggtccaccac caccaccacc actaaggatc cggggttggg gttgcgcctt ttccaaggca
     2161 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
     2221 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
     2281 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
     2341 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
     2401 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
     2461 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
     2521 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
     2581 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
     2641 agcaattcac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     2701 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     2761 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     2821 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     2881 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     2941 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     3001 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     3061 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     3121 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     3181 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     3241 ccggtgccga aggtagaggt tctctcctca cttgtggtga tgttgaagaa aaccctggtc
     3301 caatgtctag actggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg
     3361 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     3421 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     3481 tggacaggca tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc
     3541 ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     3601 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     3661 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     3721 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     3781 cacctaccac cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc
     3841 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     3901 agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc
     3961 tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg
     4021 attttgacct tgacatgctc cccgggtaac taagtaagga tcgatccaag atatcgtatt
     4081 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4141 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4201 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4261 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4321 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4381 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     4441 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4501 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4561 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4621 ccgcctgttt cgcctcggcg tccggactag aggtaccttt aagaccaatg acttacaagg
     4681 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4741 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     4801 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4861 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4921 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     4981 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     5041 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5101 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     5161 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5221 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     5281 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctttcaa atatgtatcc
     5341 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5401 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5461 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5521 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5581 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5641 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5701 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5761 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5821 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5881 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5941 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6001 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6061 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6121 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6181 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6241 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6301 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6361 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6421 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6481 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6541 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6601 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6661 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6721 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6781 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6841 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6901 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6961 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7021 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7081 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7141 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     7201 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     7261 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     7321 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     7381 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     7441 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     7501 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     7561 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     7621 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     7681 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     7741 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7801 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7861 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     7921 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     7981 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     8041 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     8101 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8161 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     8221 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     8281 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8341 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     8401 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     8461 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     8521 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     8581 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     8641 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     8701 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     8761 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     8821 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     8881 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     8941 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     9001 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     9061 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     9121 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     9181 ccga
//
