LOCUS       sequence-392795-        6978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pTatABC with an mCherry fluorescent protein tag at the end of TatB..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Sharma A, Chowdhury R, Musser SM
  TITLE     Oligomerization state of the functional bacterial twin-arginine 
            translocation (Tat) receptor complex.
  JOURNAL   Commun Biol. 2022 Sep 19;5(1):988. doi: 10.1038/s42003-022-03952-2.
  PUBMED    36123532
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-19"; volume: "5(1):988. doi"; pages: " 
            10.1038/s42003-022-03952-2"
COMMENT     Sequence Label: pBAD_TatABmCherryC
FEATURES             Location/Qualifiers
     source          1..6978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(96..974)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQFGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1001..1285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1208..1227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     regulatory      2184..2193
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2190..2900
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2335..2353)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2603..2622
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(3836..3853)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4006..4092
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4184..4211
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4230..4321
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4322..4390,4391..5182)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4540..4559)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5224..5679
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5311..5330)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5521..5542
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      5790..6378
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6279..6298
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6532..6549
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     6715..6737
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6875..6894
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatcccgaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca
     1321 tggccgtgta acgtataatg cggctttgtt taatcatcat ctaccacaga ggaacatgta
     1381 tgggtggtat cagtatttgg cagttattga ttattgccgt catcgttgta ctgctttttg
     1441 gcaccaaaaa gctcggctcc atcggttccg atcttggtgc gtcgatcaaa ggctttaaaa
     1501 aagcaatgag cgatgatgaa ccaaagcagg ataaaaccag tcaggatgct gattttactg
     1561 cgaaaactat cgccgataag caggcggata cgaatcagga acaggctaaa acagaagacg
     1621 cgaagcgcca cgataaagag caggtgtaat ccgtgtttga tatcggtttt agcgaactgc
     1681 tattggtgtt catcatcggc ctcgtcgttc tggggccgca acgactgcct gtggcggtaa
     1741 aaacggtagc gggctggatt cgcgcgttgc gttcactggc gacaacggtg cagaacgaac
     1801 tgacccagga gttaaaactc caggagtttc aggacagtct gaaaaaggtt gaaaaggcga
     1861 gcctcactaa cctgacgccc gaactgaaag cgtcgatgga tgaactacgc caggccgcgg
     1921 agtcgatgaa gcgttcctac gttgcaaacg atcctgaaaa ggcgagcgat gaagcgcaca
     1981 ccatccataa cccggtggtg aaagataatg aagctgcgca tgagggcgta acgcctgccg
     2041 ctgcacaaac gcaggccagt tcgccggaac agaagccaga aaccacgcca gagccggtgg
     2101 taaaacctgc tgcggacgct gaaccgaaaa ccgctgcacc ttccccttcg tcgagtgata
     2161 aaccgtatac ccgggtaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg
     2221 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     2281 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     2341 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     2401 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     2461 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     2521 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     2581 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     2641 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     2701 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     2761 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     2821 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     2881 tggacgagct gtacaagtag cggccgcgac tctagaattc caactgagcg ccggtcgcta
     2941 ccattaccaa cttgtctggt gtcaaaaata ataggcctac tagtcatgtc tgtagaagat
     3001 actcaaccgc ttatcacgca tctgattgag ctgcgtaagc gtctgctgaa ctgcattatc
     3061 gcggtgatcg tgatattcct gtgtctggtc tatttcgcca atgacatcta tcacctggta
     3121 tccgcgccat tgatcaagca gttgccgcaa ggttcaacga tgatcgccac cgacgtggcc
     3181 tcgccgttct ttacgccgat caagctgacc tttatggtgt cgctgattct gtcagcgccg
     3241 gtgattctct atcaggtgtg ggcatttatc gccccagcgc tgtataagca tgaacgtcgc
     3301 ctggtggtgc cgctgctggt ttccagctct ctgctgtttt atatcggcat ggcattcgcc
     3361 tactttgtgg tctttccgct ggcatttggc ttccttgcca ataccgcgcc ggaaggggtg
     3421 caggtatcca ccgacatcgc cagctattta agcttcgtta tggcgctgtt tatggcgttt
     3481 ggtgtctcct ttgaagtgcc ggtagcaatt gtgctgctgt gctggatggg gattacctcg
     3541 ccagaagact tacgcaaaaa acgcccgtat gtgctggttg gtgcattcgt tgtcgggatg
     3601 ttgctgacgc cgccggatgt cttctcgcaa acgctgttgg cgatcccgat gtactgtctg
     3661 tttgaaatcg gtgtcttctt ctcacgcttt tacgttggta aagggcgaaa tcgggaagag
     3721 gaaaacgacg ctgaagcaga aagcgaaaaa actgaagaat aaattcaacc gcctctagag
     3781 tcgacctgca ggcatgcaag cttggctgtt ttggcggatg agagaagatt ttcagcctga
     3841 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta
     3901 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
     3961 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
     4021 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     4081 agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     4141 cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg
     4201 gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat
     4261 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4321 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4381 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4441 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4501 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4561 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4621 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4681 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4741 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4801 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4861 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4921 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4981 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5041 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5101 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5161 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5221 tttacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5281 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5341 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5401 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     5461 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5521 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     5581 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5641 ttaacgcgaa ttttaacaaa atattaacgt ttacaattta aaaggatcta ggtgaagatc
     5701 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5761 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5821 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5881 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     5941 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6001 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6061 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6121 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6181 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6241 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6301 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6361 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6421 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     6481 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     6541 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     6601 atttcacacc gcatagggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     6661 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     6721 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag aaggagatgg
     6781 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     6841 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     6901 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatctgct
     6961 catgtttgac agcttatc
//
