LOCUS       sequence-392800-       15193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A pVS1-based T-DNA binary vector and destination vector for Gateway 
            cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15193)
  AUTHORS   Lee K, Kang M, Ji Q, Grosic S, Wang K
  TITLE     New T-DNA binary vectors with NptII selection and RUBY reporter for 
            efficient maize transformation and targeted mutagenesis.
  JOURNAL   Plant Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231.
  PUBMED    37070560
REFERENCE   2  (bases 1 to 15193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2023 Apr 18:kiad231. doi: 10.1093/plphys/kiad231."
COMMENT     Sequence Label: pKL2351
FEATURES             Location/Qualifiers
     source          1..15193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        544..889
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     832..852
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             959..1663
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMEELY"
     primer_bind     1372..1391
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        2193..4188
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     protein_bind    4231..4355
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4380..4385,4386..4403,4404..4410)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4464..5144
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(4530..4549)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5464..5769
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5648..5667
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5810..5934)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     gap             6381..6396
                     /estimated_length=16
     protein_bind    7414..7461
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             7481..8275
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7535..7554)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8145..8164
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8662..8836
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    8914..8938
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9363..10157
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10244..10832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10733..10752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10986..11003
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11018..11158
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11084..11103)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11244..11266
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      11502..11696
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11762..12835)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(13264..13893)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
      121 agggcgatcg ccttgggccc ttagtattcc ctacccctac tccaaaaatg tcaaagatac
      181 agtctcagaa gaccaaaggg ctattgagac ttttcaacaa agggtaattt cgggaaacct
      241 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
      301 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atgcctctgc
      361 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      421 tccaaccacg tcttcaaagc aagtggattg atgtgacatc tccactgacg taagggatga
      481 cgcacaatcc cacccctact ccaaaaatgt caaagataca gtctcagaag accaaagggc
      541 tattgagact tttcaacaaa gggtaatttc gggaaacctc ctcggattcc attgcccagc
      601 tatctgtcac ttcatcgaaa ggacagtaga aaaggaaggt ggctcctaca aatgccatca
      661 ttgcgataaa ggaaaggcta tcattcaaga tgcctctgcc gacagtggtc ccaaagatgg
      721 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
      781 agtggattga tgtgacatct ccactgacgt aagggatgac gcacaatccc actatccttc
      841 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacag cccagatcac
      901 tagtcttaag tccggagcta gctctagaga cgtctcgagg accggtcccg ggggatccat
      961 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     1021 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1081 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     1141 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     1201 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     1261 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     1321 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     1381 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     1441 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     1501 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     1561 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     1621 cgccgagggc cgccactcca ccggcggcat ggaggagctg tacagatctt aaagcggtag
     1681 ctagagtttg ctcctatcta tatgtaataa ggtatgctga tatgcactat tcaaatagga
     1741 gcattagcta tgtttgttaa tgtcacttta tgttatgtgg gtaagtcacc taagacactc
     1801 cacgtaccta cttgttgtct cttacgcggc tttaataaat cttctgccct tgttccatat
     1861 ttactaatta tccctttctt cactaaaaga aaattgttat cattaagtat tagtctttag
     1921 aacatatgag gtctttaatt gggtaggttt tacaaattaa ctaatataaa atgtcataaa
     1981 atccacgtgg ttaaacaaat gcagaaaatc gacgtcgtct attggaccga cagttgctat
     2041 taatataatg ggccaccata gtagactgac aaataaatta cctgacaaca tcgtttcaca
     2101 aaaaaacaaa cacaaaaagg gagtgcattt tccagggcat ttttgtaata aaaaacagtt
     2161 aaaagggagt gcaataagcg aagcttgcat gcctgcagtg cagcgtgacc cggtcgtgcc
     2221 cctctctaga gataatgagc attgcatgtc taagttataa aaaattacca catatttttt
     2281 ttgtcacact tgtttgaagt gcagtttatc tatctttata catatattta aactttactc
     2341 tacgaataat ataatctata gtactacaat aatatcagtg ttttagagaa tcatataaat
     2401 gaacagttag acatggtcta aaggacaatt gagtattttg acaacaggac tctacagttt
     2461 tatcttttta gtgtgcatgt gttctccttt ttttttgcaa atagcttcac ctatataata
     2521 cttcatccat tttattagta catccattta gggtttaggg ttaatggttt ttatagacta
     2581 atttttttag tacatctatt ttattctatt ttagcctcta aattaagaaa actaaaactc
     2641 tattttagtt tttttattta ataatttaga tataaaatag aataaaataa agtgactaaa
     2701 aattaaacaa atacccttta agaaattaaa aaaactaagg aaacattttt cttgtttcga
     2761 gtagataatg ccagcctgtt aaacgccgtc gacgagtcta acggacacca accagcgaac
     2821 cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg catctctgtc gctgcctctg
     2881 gacccctctc gagagttccg ctccaccgtt ggacttgctc cgctgtcggc atccagaaat
     2941 tgcgtggcgg agcggcagac gtgagccggc acggcaggcg gcctcctcct cctctcacgg
     3001 caccggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc
     3061 cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc
     3121 acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg
     3181 ccgctcgtcc tccccccccc ccctctctac cttctctaga tcggcgttcc ggtccatgca
     3241 tggttagggc ccggtagttc tacttctgtt catgtttgtg ttagatccgt gtttgtgtta
     3301 gatccgtgct gctagcgttc gtacacggat gcgacctgta cgtcagacac gttctgattg
     3361 ctaacttgcc agtgtttctc tttggggaat cctgggatgg ctctagccgt tccgcagacg
     3421 ggatcgattt catgattttt tttgtttcgt tgcatagggt ttggtttgcc cttttccttt
     3481 atttcaatat atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt
     3541 ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa ttctgtttca
     3601 aactacctgg tggatttatt aattttggat ctgtatgtgt gtgccataca tattcatagt
     3661 tacgaattga agatgatgga tggaaatatc gatctaggat aggtatacat gttgatgcgg
     3721 gttttactga tgcatataca gagatgcttt ttgttcgctt ggttgtgatg atgtggtgtg
     3781 gttgggcggt cgttcattcg ttctagatcg gagtagaata ctgtttcaaa ctacctggtg
     3841 tatttattaa ttttggaact gtatgtgtgt gtcatacatc ttcatagtta cgagtttaag
     3901 atggatggaa atatcgatct aggataggta tacatgttga tgtgggtttt actgatgcat
     3961 atacatgatg gcatatgcag catctattca tatgctctaa ccttgagtac ctatctatta
     4021 taataaacaa gtatgtttta taattatttt gatcttgata tacttggatg atggcatatg
     4081 cagcagctat atgtggattt ttttagccct gccttcatac gctatttatt tgcttggtac
     4141 tgtttctttt gtcgatgctc accctgttgt ttggtgttac ttctgcaggt cgactctaga
     4201 ggatcccctc gaggcgcgcc aagctatcaa acaagtttgt acaaaaaagc tgaacgagaa
     4261 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
     4321 taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct
     4381 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat
     4441 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
     4501 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
     4561 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
     4621 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
     4681 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
     4741 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
     4801 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
     4861 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
     4921 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
     4981 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
     5041 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
     5101 atgagtggca ggcggggcgt aatctagagg atccggctta ctaaaagcca gataacagta
     5161 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     5221 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     5281 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     5341 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     5401 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     5461 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     5521 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     5581 cgtctgctgt cagataaagt cccccgtgaa ctttacccgg tggtgcatat cggggatgaa
     5641 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     5701 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     5761 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     5821 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     5881 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttcgata
     5941 attcttaatt aactagttct agagcggccg ccaccgcggt ggatagttcc ggacttaccg
     6001 gcgccgctag cctgcagtgc agcgtgaccc ggtcgtgccc ctctctagag ataatgagca
     6061 ttgcatgtct aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg
     6121 cagtttatct atctttatac atatatttaa actttactct acgaataata taatctatag
     6181 tactacaata atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa
     6241 aggacaattg agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg
     6301 ttctcctttt tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac
     6361 atccatttag ggtttagggt nnnnnnnnnn nnnnnncccc tctctacctt ctctagatcg
     6421 gcgttccggt ccatggttag ggcccggtag ttctacttct gttcatgttt gtgttagatc
     6481 cgtgtttgtg ttagatccgt gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga
     6541 cacgttctga ttgctaactt gccagtgttt ctctttgggg aatcctggga tggctctagc
     6601 cgttccgcag acgggatcga tttcatgatt ttttttgttt cgttgcatag ggtttggttt
     6661 gcccttttcc tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgc
     6721 ttttttttgt cttggttgtg atgatgtggt ctggttgggc ggtcgttcta gatcggagta
     6781 gaattaattc tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg
     6841 ccatacatat tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg
     6901 tatacatgtt gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt
     6961 tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc tagatcggag tagaatactg
     7021 tttcaaacta cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc
     7081 atagttacga gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt
     7141 gggttttact gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct
     7201 tgagtaccta tctattataa taaacaagta tgttttataa ttattttgat cttgatatac
     7261 ttggatgatg gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct
     7321 atttatttgc ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc
     7381 tgcaggtcga ctctagagga tcaattcgct agcgaagttc ctattccgaa gttcctattc
     7441 tctagaaagt ataggaactt cagatccacc gggatccacc atggttgaac aagatggatt
     7501 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     7561 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     7621 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     7681 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     7741 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     7801 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     7861 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     7921 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     7981 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgatgatc tcgtcgtgac
     8041 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     8101 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     8161 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     8221 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaggatc
     8281 caccatggtt aacctagact tgtccatctt ctggattggc caacttaatt aatgtatgaa
     8341 ataaaaggat gcacacatag tgacatgcta atcactataa tgtgggcatc aaagttgtgt
     8401 gttatgtgta attactagtt atctgaataa aagagaaaga gatcatccat atttcttatc
     8461 ctaaatgaat gtcacgtgtc tttataattc tttgatgaac cagatgcatt tcattaacca
     8521 aatccatata catataaata ttaatcatat ataattaata tcaattgggt tagcaaaaca
     8581 aatctagtct aggtgtgttt tgcaagcttg catgcctgca cgccccgtcc ggtcctgccc
     8641 gtcaccgaga tttgactcga gtttctccat aataatgtgt gagtagttcc cagataaggg
     8701 aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt
     8761 atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag
     8821 tactaaaatc cagatccccc gaattaattc ggcgttaatt cagtacatta aaaacgtccg
     8881 caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc
     8941 agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag
     9001 cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat
     9061 gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc
     9121 tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaccgc
     9181 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     9241 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     9301 ttgttataat tagcttcttg gggtatcttt aaatactgta gaaaagagga aggaaataat
     9361 aaatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg
     9421 taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa
     9481 acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg
     9541 aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg
     9601 aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg
     9661 aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca
     9721 ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct
     9781 tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg
     9841 aagaagacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc
     9901 ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag
     9961 atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg
    10021 acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc
    10081 tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac
    10141 tggatgaatt gttttagtac ctagaatgca tgaccaaaat cccttaacgt gagttttcgt
    10201 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    10261 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    10321 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    10381 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    10441 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    10501 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    10561 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    10621 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10681 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10741 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10801 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10861 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    10921 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    10981 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
    11041 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
    11101 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
    11161 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
    11221 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
    11281 accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg
    11341 cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag
    11401 cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag
    11461 gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg
    11521 ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt
    11581 ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt
    11641 acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa
    11701 agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac
    11761 attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg
    11821 ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca
    11881 gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga
    11941 tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga
    12001 gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg
    12061 ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact
    12121 ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg
    12181 ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg
    12241 tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc
    12301 ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct
    12361 tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt
    12421 cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg
    12481 gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg
    12541 ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa
    12601 aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg
    12661 gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg
    12721 cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt
    12781 catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga
    12841 ttcctcgggc ttgggggttc cagtgccatt gcagggccgg caggcaaccc agccgcttac
    12901 gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg
    12961 ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt
    13021 tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc
    13081 cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga
    13141 cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg
    13201 cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt
    13261 aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc
    13321 gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac
    13381 gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac
    13441 ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct
    13501 tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat
    13561 gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac
    13621 agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc
    13681 cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc
    13741 ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc
    13801 ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc
    13861 gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt
    13921 actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat
    13981 caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc
    14041 agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg
    14101 ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa
    14161 aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct
    14221 cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc
    14281 tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat
    14341 gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc
    14401 gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg
    14461 ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg
    14521 caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg
    14581 gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta
    14641 ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc
    14701 gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct
    14761 cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct
    14821 ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg
    14881 cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa
    14941 gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca
    15001 tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga
    15061 ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat gcacatacaa
    15121 atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc taataaacgc
    15181 tcttttctct tag
//
