LOCUS       sequence-392811-        6845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pTatABC with an mNeonGreen fluorescent protein tag at the C-terminal
            end of TatC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Sharma A, Chowdhury R, Musser SM
  TITLE     Oligomerization state of the functional bacterial twin-arginine 
            translocation (Tat) receptor complex.
  JOURNAL   Commun Biol. 2022 Sep 19;5(1):988. doi: 10.1038/s42003-022-03952-2.
  PUBMED    36123532
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-09-19"; volume: "5(1):988. doi"; pages: " 
            10.1038/s42003-022-03952-2"
COMMENT     Sequence Label: pTatABCmNeon
FEATURES             Location/Qualifiers
     source          1..6845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(96..974)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1001..1285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1208..1227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     regulatory      3203..3212
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3703..3720)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3873..3959
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4051..4078
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4097..4188
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4189..4257,4258..5049)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4407..4426)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5091..5546
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5178..5197)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5388..5409
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      5657..6245
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6146..6165
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6399..6416
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     6582..6604
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6742..6761
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
     1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
     1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaggag gaattcacca
     1321 tgggacggct ttgtttaatc atcatctacc acagaggaac atgtatgggt ggtatcagta
     1381 tttggcagtt attgattatt gccgtcatcg ttgtactgct ttttggcacc aaaaagctcg
     1441 gctccatcgg ttccgatctt ggtgcgtcga tcaaaggctt taaaaaagca atgagcgatg
     1501 atgaaccaaa gcaggataaa accagtcagg atgctgattt tactgcgaaa actatcgccg
     1561 ataagcaggc ggatacgaat caggaacagg ctaaaacaga agacgcgaag cgccacgata
     1621 aagagcaggt gggtacctaa tccgtgtttg atatcggttt tagcgaactg ctattggtgt
     1681 tcatcatcgg cctcgtcgtt ctggggccgc aacgactgcc tgtggcggta aaaacggtag
     1741 cgggctggat tcgcgcgttg cgttcactgg cgacaacggt gcagaacgaa ctgacccagg
     1801 agttaaaact ccaggagttt caggacagtc tgaaaaaggt tgaaaaggcg agcctcacta
     1861 acctgacgcc cgaactgaaa gcgtcgatgg atgaactacg ccaggccgcg gagtcgatga
     1921 agcgttccta cgttgcaaac gatcctgaaa aggcgagcga tgaagcgcac accatccata
     1981 acccggtggt gaaagataat gaagctgcgc atgagggcgt aacgcctgcc gctgcacaaa
     2041 cgcaggccag ttcgccggaa cagaagccag aaaccacgcc agagccggtg gtaaaacctg
     2101 ctgcggacgc tgaaccgaaa accgctgcac cttccccttc gtcgagtgat aaaccgtcta
     2161 gataaacatg tctgtagaag atactcaacc gcttatcacg catctgattg agctgcgtaa
     2221 gcgtctgctg aactgcatta tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc
     2281 caatgacatc tatcacctgg tatccgcgcc attgatcaag cagttgccgc aaggttcaac
     2341 gatgatcgcc accgacgtgg cctcgccgtt ctttacgccg atcaagctga cctttatggt
     2401 gtcgctgatt ctgtcagcgc cggtgattct ctatcaggtg tgggcattta tcgccccagc
     2461 gctgtataag catgaacgtc gcctggtggt gccgctgctg gtttccagct ctctgctgtt
     2521 ttatatcggc atggcattcg cctactttgt ggtctttccg ctggcatttg gcttccttgc
     2581 caataccgcg ccggaagggg tgcaggtatc caccgacatc gccagctatt taagcttcgt
     2641 tatggcgctg tttatggcgt ttggtgtctc ctttgaagtg ccggtagcaa ttgtgctgct
     2701 gtgctggatg gggattacct cgccagaaga cttacgcaaa aaacgcccgt atgtgctggt
     2761 tggtgcattc gttgtcggga tgttgctgac gccgccggat gtcttctcgc aaacgctgtt
     2821 ggcgatcccg atgtactgtc tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg
     2881 taaagggcga aatcgggaag aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga
     2941 agtcgacatg gtgtccaagg gcgaagagga caacatggcc agcctgcctg ccacccacga
     3001 gctgcacatc ttcggcagca tcaacggcgt ggacttcgac atggtgggac agggcaccgg
     3061 caaccccaac gacggctacg aggaactgaa cctgaagtcc acaaagggcg acctgcagtt
     3121 cagcccctgg attctggtgc cccacatcgg ctacggcttc caccagtacc tgccctaccc
     3181 cgacggcatg agccctttcc aagccgccat ggtggatggc agcggctacc aggtgcaccg
     3241 gaccatgcag tttgaggacg gcgccagcct gaccgtgaac tacaggtaca catacgaggg
     3301 cagccacatc aagggcgagg cccaagtgaa gggcacaggc tttccagccg acggccccgt
     3361 gatgaccaat agcctgacag ccgccgactg gtgcagaagc aagaaaacct accccaatga
     3421 caagaccatc atcagcacct tcaagtggtc ctacaccacc ggcaatggca agcggtacag
     3481 aagcaccgcc cggaccacct acaccttcgc caaacccatg gccgccaact acctgaagaa
     3541 ccagcctatg tacgtgttcc gcaagaccga gctgaagcac tccaagacag aactgaactt
     3601 caaagagtgg cagaaagcct tcaccgacgt gatgggcatg gacgagctgt acaagtgagc
     3661 atgcaagctt ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc
     3721 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc
     3781 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
     3841 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag
     3901 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     3961 cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc
     4021 cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg
     4081 cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4141 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4201 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4261 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4321 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4381 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     4441 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4501 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4561 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4621 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4681 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     4741 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4801 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4861 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4921 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4981 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5041 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt acgcgccctg
     5101 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     5161 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     5221 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     5281 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     5341 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     5401 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
     5461 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     5521 taacaaaata ttaacgttta caatttaaaa ggatctaggt gaagatcctt tttgataatc
     5581 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5641 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5701 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5761 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     5821 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     5881 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     5941 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6001 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6061 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6121 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6181 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6241 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6301 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6361 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     6421 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     6481 tagggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     6541 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     6601 gaggttttca ccgtcatcac cgaaacgcgc gaggcagaag gagatggcgc ccaacagtcc
     6661 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     6721 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     6781 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atctgctcat gtttgacagc
     6841 ttatc
//
