LOCUS       sequence-392888-        9637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway compatible Y2H vector - AD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Lu Q, Tang X, Tian G, Wang F, Liu K, Nguyen V, Kohalmi SE, Keller 
            WA, Tsang EW, Harada JJ, Rothstein SJ, Cui Y
  TITLE     Arabidopsis homolog of the yeast TREX-2 mRNA export complex: 
            components and anchoring nucleoporin.
  JOURNAL   Plant J. 2010 Jan;61(2):259-70. doi: 
            10.1111/j.1365-313X.2009.04048.x. Epub 2009 Oct 16.
  PUBMED    19843313
REFERENCE   2  (bases 1 to 9637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-313X.2009.04048.x"; journalName: "Plant J"; date: 
            "2010-01"; volume: "61"; issue: "2"; pages: "259-70"
COMMENT     Sequence Label: pGADT7-GW
FEATURES             Location/Qualifiers
     source          1..9637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        771..1475
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1521..1541
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1557..1898
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     1904..1923
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1904..1922
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1929..1938
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1941..1967
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1974..2098
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2123..2128,2129..2146,2147..2153)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2207..2866
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2273..2292)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3208..3513
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3392..3411
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3554..3678)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4065..4252
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4120..4144)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(4369..5463)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5464..5869)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(5911..5927)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5911..5927)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5924..5946)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5935..5951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5959..5965,5966..5983,5984..5989))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6004..6025
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    6080..6113
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    6191..6224
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6293..6310)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6464..7052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6544..6563)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7223..8014,8015..8083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7846..7865
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8084..8188)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8256..8274
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8312..8334)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8434..8453
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      8470..9634
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
       61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
      121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
      181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
      241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
      301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
      361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
      421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
      481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
      541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
      601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
      661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
      721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
      781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
      841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
      901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
      961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata
     1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca
     1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat
     1141 gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag
     1201 atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct
     1261 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg
     1321 aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat
     1381 taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca
     1441 caatatttca agctatacca agcatacaat caactccaag ctttgcaaag atggataaag
     1501 cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca
     1561 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta
     1621 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg
     1681 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg
     1741 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt
     1801 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat
     1861 tcgatgatga agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata
     1921 gggcgagcgc cgccatggag tacccatacg acgtaccaga ttacgctcat atgacaagtt
     1981 tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
     2041 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatggc
     2101 ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat
     2161 tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
     2221 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
     2281 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
     2341 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
     2401 cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg
     2461 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
     2521 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
     2581 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
     2641 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
     2701 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
     2761 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
     2821 taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg
     2881 cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat
     2941 atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt
     3001 acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct
     3061 ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga
     3121 aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt
     3181 ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag
     3241 agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg
     3301 atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac
     3361 ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg
     3421 ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa
     3481 aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag
     3541 tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
     3601 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc
     3661 tttcttgtac aaagtggtct cgagctgcag atgaatcgta gatactgaaa aaccccgcaa
     3721 gttcacttca actgtgcatc gtgcaccatc tcaatttctt tcatttatac atcgttttgc
     3781 cttcttttat gtaactatac tcctctaagt ttcaatcttg gccatgtaac ctctgatcta
     3841 tagaattttt taaatgacta gaattaatgc ccatcttttt tttggaccta aattcttcat
     3901 gaaaatatat tacgagggct tattcagaag ctttggactt cttcgccaga ggtttggtca
     3961 agtctccaat caaggttgtc ggcttgtcta ccttgccaga aatttacgaa aagatggaaa
     4021 agggtcaaat cgttggtaga tacgttgttg acacttctaa ataagcgaat ttcttatgat
     4081 ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg
     4141 actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg taggtcaggt
     4201 tgctttctca ggtatagcat gaggtcgctc ttattgacca cacctctacc ggccggtcga
     4261 aattccccta ccctatgaac atattccatt ttgtaatttc gtgtcgtttc tattatgaat
     4321 ttcatttata aagtttatgt acaaatatca taaaaaaaga gaatcttttt aagcaaggat
     4381 tttcttaact tcttcggcga cagcatcacc gacttcggtg gtactgttgg aaccacctaa
     4441 atcaccagtt ctgatacctg catccaaaac ctttttaact gcatcttcaa tggccttacc
     4501 ttcttcaggc aagttcaatg acaatttcaa catcattgca gcagacaaga tagtggcgat
     4561 agggttgacc ttattctttg gcaaatctgg agcagaaccg tggcatggtt cgtacaaacc
     4621 aaatgcggtg ttcttgtctg gcaaagaggc caaggacgca gatggcaaca aacccaagga
     4681 acctgggata acggaggctt catcggagat gatatcacca aacatgttgc tggtgattat
     4741 aataccattt aggtgggttg ggttcttaac taggatcatg gcggcagaat caatcaattg
     4801 atgttgaacc ttcaatgtag gaaattcgtt cttgatggtt tcctccacag tttttctcca
     4861 taatcttgaa gaggccaaaa cattagcttt atccaaggac caaataggca atggtggctc
     4921 atgttgtagg gccatgaaag cggccattct tgtgattctt tgcacttctg gaacggtgta
     4981 ttgttcacta tcccaagcga caccatcacc atcgtcttcc tttctcttac caaagtaaat
     5041 acctcccact aattctctga caacaacgaa gtcagtacct ttagcaaatt gtggcttgat
     5101 tggagataag tctaaaagag agtcggatgc aaagttacat ggtcttaagt tggcgtacaa
     5161 ttgaagttct ttacggattt ttagtaaacc ttgttcaggt ctaacactac ctgtacccca
     5221 tttaggacca cccacagcac ctaacaaaac ggcatcagcc ttcttggagg cttccagcgc
     5281 ctcatctgga agtgggacac ctgtagcttc gatagcagca ccaccaatta aatgattttc
     5341 gaaatcgaac ttgacattgg aacgaacatc agaaatagct ttaagaacct taatggcttc
     5401 ggctgtgatt tcttgaccaa cgtggtcacc tggcaaaacg acgatcttct taggggcaga
     5461 cattagaatg gtatatcctt gaaatatata tatatattgc tgaaatgtaa aaggtaagaa
     5521 aagttagaaa gtaagacgat tgctaaccac ctattggaaa aaacaatagg tccttaaata
     5581 atattgtcaa cttcaagtat tgtgatgcaa gcatttagtc atgaacgctt ctctattcta
     5641 tatgaaaagc cggttccggc gctctcacct ttcctttttc tcccaatttt tcagttgaaa
     5701 aaggtatatg cgtcaggcga cctctgaaat taacaaaaaa tttccagtca tcgaatttga
     5761 ttctgtgcga tagcgcccct gtgtgttctc gttatgttga ggaaaaaaat aatggttgct
     5821 aagagattcg aactcttgca tcttacgata cctgagtatt cccacagttg ggggatctcg
     5881 actctagcta gaggatcaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
     5941 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6001 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6061 aaacctgtcg tgccagctga taacttcgta taatgtatgc tatacgaagt tattaggtct
     6121 gaagaggagt ttacgtccag ccaagctagc ttggctgcag gtcgagcggc cgcgatccgg
     6181 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttatcagctg cattaatgaa
     6241 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6301 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6361 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6421 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6481 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6541 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6601 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6661 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6721 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6781 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6841 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6901 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6961 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7021 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7081 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7141 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7201 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7261 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7321 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7381 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7441 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7501 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7561 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7621 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7681 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7741 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7801 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7861 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7921 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7981 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8041 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8101 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8161 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     8221 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8281 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8341 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8401 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     8461 tgcaccataa cgcatttaag cataaacacg cactatgccg ttcttctcat gtatatatat
     8521 atacaggcaa cacgcagata taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg
     8581 cgttgcattt tcggaagcgc tcgttttcgg aaacgctttg aagttcctat tccgaagttc
     8641 ctattctcta gctagaaagt ataggaactt cagagcgctt ttgaaaacca aaagcgctct
     8701 gaagacgcac tttcaaaaaa ccaaaaacgc accggactgt aacgagctac taaaatattg
     8761 cgaataccgc ttccacaaac attgctcaaa agtatctctt tgctatatat ctctgtgcta
     8821 tatccctata taacctaccc atccaccttt cgctccttga acttgcatct aaactcgacc
     8881 tctacatttt ttatgtttat ctctagtatt actctttaga caaaaaaatt gtagtaagaa
     8941 ctattcatag agtgaatcga aaacaatacg aaaatgtaaa catttcctat acgtagtata
     9001 tagagacaaa atagaagaaa ccgttcataa ttttctgacc aatgaagaat catcaacgct
     9061 atcactttct gttcacaaag tatgcgcaat ccacatcggt atagaatata atcggggatg
     9121 cctttatctt gaaaaaatgc acccgcagct tcgctagtaa tcagtaaacg cgggaagtgg
     9181 agtcaggctt tttttatgga agagaaaata gacaccaaag tagccttctt ctaaccttaa
     9241 cggacctaca gtgcaaaaag ttatcaagag actgcattat agagcgcaca aaggagaaaa
     9301 aaagtaatct aagatgcttt gttagaaaaa tagcgctctc gggatgcatt tttgtagaac
     9361 aaaaaagaag tatagattct ttgttggtaa aatagcgctc tcgcgttgca tttctgttct
     9421 gtaaaaatgc agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt
     9481 tttacaaaaa tgaagcacag attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct
     9541 gttctgtaaa aatgcagctc agattctttg tttgaaaaat tagcgctctc gcgttgcatt
     9601 tttgttctac aaaatgaagc acagatgctt cgttgct
//
