LOCUS       sequence-39289-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  TITLE     Akt
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 1227 pcDNA3 Myr HA Akt2
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             77..118
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             122..148
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YAYDVPDYA"
     CDS             128..154
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttccc accaccatgg ggagcagcaa gagcaagccc aaggacccca gccagcgcgc
      121 ctacgcctac gacgtgcccg actacgcctc cctcggatcc atgaatgagg tgtctgtcat
      181 caaagaaggc tggctccaca agcgtggtga atacatcaag acctggaggc cacggtactt
      241 cctgctgaag agcgacggct ccttcattgg gtacaaggag aggcccgagg cccctgatca
      301 gactctaccc cccttaaaca acttctccgt agcagaatgc cagctgatga agaccgagag
      361 gccgcgaccc aacacctttg tcatacgctg cctgcagtgg accacagtca tcgagaggac
      421 cttccacgtg gattctccag acgagaggga ggagtggatg cgggccatcc agatggtcgc
      481 caacagcctc aagcagcggg ccccaggcga ggaccccatg gactacaagt gtggctcccc
      541 cagtgactcc tccacgactg aggagatgga agtggcggtc agcaaggcac gggctaaagt
      601 gaccatgaat gacttcgact atctcaaact ccttggcaag ggaacctttg gcaaagtcat
      661 cctggtgcgg gagaaggcca ctggccgcta ctacgccatg aagatcctgc ggaaggaagt
      721 catcattgcc aaggatgaag tcgctcacac agtcaccgag agccgggtcc tccagaacac
      781 caggcacccg ttcctcactg cgctgaagta tgccttccag acccacgac
//
