LOCUS       sequence-392893-        6711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing an antigen targeting the GABA-A Receptor delta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6711)
  TITLE     Trimmer lab antigen plasmids
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GabaARD-GFP/pCDNA
FEATURES             Location/Qualifiers
     source          1..6711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      578..1166
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1067..1086
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1320..1337
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1454..1475
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1490..1495,1496..1513,1514..1520)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1528..1544
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1533..1555
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1552..1568
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1552..1568
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        1597..1976
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1977..2180
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2131..2151
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2177..2201
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2411..2429
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2411..2428
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2442..2461
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(2625..2653)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3248..3268
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     regulatory      4594..4603
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4607..4741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4644..4663)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4698..4717
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        4810..5167
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4810..5001
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4833..4853)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5018..5153
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5187..5204)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5187..5203)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5196..5218)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5416..5871
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5503..5522)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5713..5734
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5888..5907)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6007..6029
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6067..6085)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6153..6257
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6258..6326,6327..6711,1..407)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6476..6495)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
       61 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
      121 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
      181 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
      241 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
      301 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
      361 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
      421 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
      481 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
      541 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      601 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      661 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      721 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      781 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      841 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      901 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      961 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1021 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1081 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1141 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1201 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1261 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     1321 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     1381 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     1441 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     1501 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     1561 gctatgacat gattacgaat taattcgagc tcgcccgaca ttgattattg actagttatt
     1621 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     1681 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     1741 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     1801 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     1861 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     1921 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     1981 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     2041 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     2101 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     2161 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
     2221 gctgttttga cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt
     2281 gcattggaac gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata
     2341 ggcccacccc cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca
     2401 tacacatacg atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg
     2461 tgtccactcc caggtccaac tgcacctcgg ttctatcgat tgaattcgcc atggacgttc
     2521 tgggctggct gctgctgccg ctccttctgc tgtgcacgca gccgcaccat ggcgccagag
     2581 caatgaatga cattgggctc gagggagaag aacttttcac tggagttgtc ccaattcttg
     2641 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     2701 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     2761 catggccaac acttgtcact actctgacct atggtgttca atgcttttca agatacccag
     2821 atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
     2881 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
     2941 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
     3001 ttcttggaca caaattggaa tacaactata acgatcacca ggtgtacatc atggcagaca
     3061 aacaaaagaa tggaatcaaa gctaacttca aaattagaca caacattgaa gatggaggcg
     3121 ttcaactagc agaccattat caacaaaata ctccaattgg cgatgggccc gtccttttac
     3181 cagacaacca ttacctgttt acaacttcta ctctttcgaa agatcccaac gaaaagagag
     3241 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     3301 tatacaaatc tagagactac gtgggctcca acctggagat atcctggctc cccaacctgg
     3361 atggactaat ggagggctac gcccgaaact tccgaccagg cattggaggt cctccagtga
     3421 atgtggcgct tgccctagag gtggccagca ttgaccacat ctcagaagca aatatggaat
     3481 acaccatgac agtgttcctg caccagagct ggcgagacag caggctgtcc tacaaccata
     3541 ccaacgagac cctgggcctg gatagccgct tcgtggacaa gctgtggctc cctgacacct
     3601 tcattgtgaa tgccaagtct gcctggttcc atgatgtgac cgtggaaaac aagcttatcc
     3661 gcctacagcc cgacggtgtg attttataca gcatccgcat cacctccaca gtggcctgtg
     3721 acatggacct tgccaagtac cccatggacg agcaggagtg catgctggac ctggagagct
     3781 atggctactc ttctgaggac attgtctatt attggtcaga aaaccaggag cagatccacg
     3841 ggctggacag gctgcaactg gcccagttca ctatcaccag ttaccgcttc accacggagc
     3901 tgatgaactt caaatcagct ggccagttcc ctcgactcag cttacacttc cagcttcgga
     3961 ggaaccgggg tgtctacatc atccagtctt acatgccctc tgtcctcctg gttgccatgt
     4021 cctgggtctc cttctggatt agccaagcag cagtgcctgc cagagtatct ctaggcatca
     4081 ccactgtgct gacaatgacc acactcatgg ttagtgcccg ctcctccctc ccgcgggctt
     4141 ctgctatcaa ggctctggat gtgtatttct ggatctgcta tgtcttcgtg tttgctgccc
     4201 tggtggagta tgcatttgcc cacttcaatg ctgactacag gaagaaacgg aaagccaagg
     4261 tcaaggtcac gaagccaagg gcagagatgg acgtgaggaa cgccattgtc ctcttctccc
     4321 tctctgctgc tggggtcagc caggagttgg ctatctcccg ccgtcaaggc cgggtccctg
     4381 ggaacctcat gggttcctat aggtctgtag aagtggaggc aaagaaggag ggggggtccc
     4441 gcccaggagg cccaggaggc atccgttcca gactcaaacc catcgatgca gacaccatcg
     4501 acatctatgc ccgcgctgtg ttcccggcag cctttgcagc agtcaacatc atctactggg
     4561 cggcgtatac catgtgacgg ctgcagaagc ttggccgcca tggcccaact tgtttattgc
     4621 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4681 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     4741 cgggaattaa ttcggcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt
     4801 aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     4861 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4921 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4981 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5041 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5101 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5161 ttgcaaaaag ctgttaacag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
     5221 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     5281 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     5341 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     5401 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5461 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5521 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5581 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     5641 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5701 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
     5761 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5821 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     5881 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     5941 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     6001 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     6061 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     6121 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6181 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6241 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6301 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6361 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6421 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6481 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6541 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6601 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6661 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac a
//
