LOCUS       sequence-392915-        6015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing an antigen targeting the GABA-A receptor alpha5 
            subunit.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6015)
  TITLE     Trimmer lab antigen plasmids
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GABAAR alpha5 HA/pCI
FEATURES             Location/Qualifiers
     source          1..6015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     459..478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        769..1148
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1149..1352
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1303..1323
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1349..1373
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1488..1620
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1603..1622
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1665..1683
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3760..3881
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3771..3790)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3825..3844
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      4062..4517
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4149..4168)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4359..4380
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4584..4603)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4703..4725
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4763..4781)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4849..4953
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4954..5022,5023..5814)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5172..5191)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(5985..6015,1..558)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
       61 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
      121 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      181 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      241 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      301 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
      361 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      421 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      481 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      541 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
      601 tggccttttg ctcacatggc tcgacagatc ttcaatattg gccattagcc atattattca
      661 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
      721 ataatatgta catttatatt ggctcatgtc caatatgacc gccatgttgg cattgattat
      781 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      841 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      901 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      961 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     1021 tgccaagtcc gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     1081 agtacatgac cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     1141 ttaccatggt gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac
     1201 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     1261 aacgggactt tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc
     1321 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcactagaa
     1381 gctttattgc ggtagtttat cacagttaaa ttgctaacgc agtcagtgct tctgacacaa
     1441 cagtctcgaa cttaagctgc agaagttggt cgtgaggcac tgggcaggta agtatcaagg
     1501 ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct
     1561 tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag
     1621 gtgtccactc ccagttcaat tacagctctt aaggctagag tacttaatac gactcactat
     1681 aggctagcct cgagaattag cttcaagatc aagttggagg aaaggacggt ttttcttcta
     1741 aattcatctg cttcaactat tattcttact gggaatggac aatggaatgc tctctagatt
     1801 tatcatgacc aaaaccctcc ttgtcttctg catttccatg accttatcca gtcactttgg
     1861 cttttcacaa atgccaacta gttctgtaca agatgagacc aatgacaaca tcacaatatt
     1921 caccaggatc ttggacgggc tcttggatgg ctatgacaac agactgcggc ctgggctggg
     1981 agagcgaatc acgcaggtgc gaacagacat ctatgttacc agctttggcc cagtgtccga
     2041 cacggaaatg gaatatacca tagatgtgtt tttccgacaa agctggaaag atgaaaggct
     2101 gcggtttaag gggcctatgc aacgtctccc tctcaacaac cttcttgcca gcaaaatctg
     2161 gaccccagac acattcttcc acaatgggaa gaagtccatt gcgcacaaca tgacgacacc
     2221 caacaagctg ctgaggctgg aggatgatgg cacacttctc tacaccatgc gcctgacgat
     2281 ctctgctgag tgtccaatgc agcttgagga cttcccgatg gatgcccatg cttgtcccct
     2341 gaaatttggc agttatgctt accctaattc ggaagttgtc tatgtttgga ccaatggttc
     2401 caccaagtct gtggtggtgg cagaagatgg ctccagactc aaccagtacc acctcatggg
     2461 gcagacagta ggcactgaga acatcagcac cagcacaggt gaatatacaa tcatgactgc
     2521 tcattttcac ctgaagagga agatcgggta ctttgtcatc cagacgtacc ttccctgcat
     2581 catgactgtc atcttatccc aggtgtcttt ttggcttaat cgagaatctg tcccagctag
     2641 gacagttttt ggagtgacca cagtgctgac catgacaacc ctcagcatca gtgcccggaa
     2701 ttcgctgccc aaagtggcct atgccacagc catggactgg ttcattgctg tctgctatgc
     2761 atttgtcttc tctgccctga ttgaatttgc cacagtcaac tactttacaa agagaggatg
     2821 ggcctgggat ggcaagaagg ccttggaagc agctaaaatc aagaaaaaag aacgtgaact
     2881 catactaaat aagtcaacaa atgcttttac aactgggaag ttgacccatc ctccaaacat
     2941 cccaaaggag cagcttccag gcgggactgg gaatgctgtg ggtacagcct caatcagagc
     3001 atctgaggag aagacttctg agagtaaaaa gacctacaac agcatcagca agatcgacaa
     3061 aatgtcccgg attgtgttcc ccattttgtt tggcactttc aatctagttt actgggcaac
     3121 atatttgaat agggagcccg tgataaaagg ggctacctct ccaaagtaag acaggaaacc
     3181 atacttgcac agaaatgaac ctgaggagag gtcaagctca cagagactat ttgggcgctg
     3241 tctttcagga aattttgcat gtttaataat atgtacaaat aatattgcct tgatgtttct
     3301 atgtgtaact tcaatgtttc aaggatgtcc ccttaataaa ccaagcaaat ggccttctac
     3361 aacaacggga ggcaatgact gactctcaga tgctcagcgt cctaacatca atagtttaca
     3421 aacaagataa gtatattttt aactgttctg agtatatgac gtttttatac ttcgaatgcc
     3481 atttcgtacc atttttccca gccaacagaa cattttaggg aatccctgtg atgaccactt
     3541 gacaggtgaa aaagcaaaga tcctcgggta cacaaagtcc atgaagagca aactgtggac
     3601 atttaagtcc agtacgaatt gcctttaaca attcttcttg ttctgaaatt agaaaaatac
     3661 tgcatgaact gacattaaga agtagaataa gcaaacattt atgccggaat tcatcgatat
     3721 ctagatctcg acccgggcgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
     3781 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     3841 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     3901 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct
     3961 acaaatgtgg taaaatcgat aaggatccgg gctggcgtaa tagcgaagag gcccgcaccg
     4021 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc
     4081 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4141 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4201 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4261 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4321 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4381 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4441 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4501 attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4561 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     4621 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     4681 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4741 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4801 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4861 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4921 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4981 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5041 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5101 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5161 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5221 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5281 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5341 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5401 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5461 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5521 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5581 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5641 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5701 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5761 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5821 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5881 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5941 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6001 ctgcgcgtaa tctgc
//
