LOCUS       sequence-392920-        9983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of Flip-nluc(LgBiT1-8)CP[CoVA] protease 
            reporter and P2A firefly luciferase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Arakawa M, Yoshida A, Okamura S, Ebina H, Morita E
  TITLE     A highly sensitive NanoLuc-based protease biosensor for detecting 
            apoptosis and SARS-CoV-2 infection.
  JOURNAL   Sci Rep. 2023 Jan 31;13(1):1753. doi: 10.1038/s41598-023-28984-4.
  PUBMED    36720982
REFERENCE   2  (bases 1 to 9983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-01-31"; volume: "13(1):1753. doi"; pages: " 
            10.1038/s41598-023-28984-4"
COMMENT     Sequence Label: pQC.Flip-nluc(LgBiT1-8)CP[CoVA]-Fluc
FEATURES             Location/Qualifiers
     source          1..9983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2279..2288
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2669..2722
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2729..2738
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3227..3274
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             3278..3397
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             3413..3469
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3476..5128
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    5132..5705
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5299..5316)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5526..5545
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5731..6330
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5731..5750)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6227..6247
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6689..7114
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7217..7239)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7375..7395)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7380..7709
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7560..7695
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7731..7750
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7831..7848)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8002..8590)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8082..8101)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8761..9552,9553..9621))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9384..9403
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9622..9726)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9794..9812
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9912..9983,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca ccggtaggcc tcgtacgctt aattaacggc
     2281 caccatgggc tccctgctgt tccgagtaac catcaacggc ggcggtggag gctccggggg
     2341 aggcgggtct ggcggagggg gaagtggcgg tggtggctct gaagttagcg ctctggaaaa
     2401 agaagtgtct gcactcgaga aagaagtaag tgcccttgag aaggaggtgt ccgcactcga
     2461 gaaggaggtc agcgccctgg aaaaggaaaa ggtgagtggg tggcgacttt tcaaaaaaat
     2521 ctccggatcc tcagtggccg tgctgcagag cggcttcaag gtgtccgccc tgaaggaaaa
     2581 agtaagcgca ctgaaagaaa aggtgagcgc gctgaaggag aaagtgagcg ccctgaaaga
     2641 gaaagtctct gcccttaagg aggaattcga gggcagagga agtctgctaa catgcggtga
     2701 cgtcgaggag aatcctggcc caaagcttgc caccatggtt ttcacattgg aagacttcgt
     2761 gggtgactgg gagcagactg ccgcgtacaa cttggatcaa gttctggagc aaggtggagt
     2821 aagctccctc ctgcaaaacc ttgcggtaag cgttactccc atccaaagga tcgtaaggtc
     2881 cggggaaaat gctctcaaaa tagatataca cgttatcata ccttacgaag ggctgtctgc
     2941 cgatcagatg gctcaaatag aagaagtttt caaagtagtc taccccgtgg acgatcatca
     3001 ttttaaggtg attctccctt atgggaccct cgttatcgac ggggttacac ctaatatgct
     3061 caattacttc ggaaggccat atgaaggaat agctgttttc gatggtaaaa agattacggt
     3121 caccggcact ctctggaatg ggaataagat aatagatgag agactgataa cgccagacgg
     3181 cgtggccgtg ctgcagagcg gcttccaatt gggaggcagc gaagtggctt gcaagaactg
     3241 gttcagtagc ttaagccact ttgtgatcca ccttaattct cacggctttc cgcctgaggt
     3301 tgaagagcaa gccgccggta cattgcctat gtcctgcgca caagaaagcg gtatggaccg
     3361 gcacccagcc gcttgtgctt cagctcgcat caacgtcggc ggccgctcta gagcaaccaa
     3421 tttcagcctt cttaaacagg ctggagatgt ggaagagaat cctggaccag aattgatgga
     3481 agatgccaaa aacattaaga agggcccagc gccattctac ccactcgaag acgggaccgc
     3541 cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt
     3601 taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg
     3661 gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgcag
     3721 cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt
     3781 ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca
     3841 gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa
     3901 gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca
     3961 aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt
     4021 gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac
     4081 cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc
     4141 ccgcgacccc atcttcggca accagatcat ccccgacacc gctatcctca gcgtggtgcc
     4201 atttcaccac ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt
     4261 cgtgctcatg taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat
     4321 tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga
     4381 caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga
     4441 ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct
     4501 gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt
     4561 aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact
     4621 gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt
     4681 taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggcga
     4741 catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat
     4801 caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc
     4861 caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc
     4921 cgcagtcgtc gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt
     4981 ggccagccag gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt
     5041 gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc
     5101 caagaagggc ggcaagatcg ccgtgtaacc gcccctctcc ctcccccccc cctaacgtta
     5161 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     5221 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     5281 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     5341 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     5401 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     5461 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     5521 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     5581 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     5641 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     5701 gataagcttg ccacaaggag acgaccttcc atgaccgagt acaagcccac ggtgcgcctc
     5761 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     5821 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     5881 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     5941 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6001 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6061 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggagtc
     6121 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6181 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6241 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6301 tggtgcatga cccgcaagcc cggtgcctga tgcttgcagg catctcatga gtgtgtcttc
     6361 ccgttttccg cctgaggtca ctgcgtggat ggagcgctgg cgcctgctgc gcgacggcga
     6421 gctgctcacc acccactcga gggctgcagc gctgcagagg ccgagtgcag aactgctcca
     6481 aagggacctc aaggctttcc gagggacact aggctgactc catcgagcca gtgtagagat
     6541 aagcttatcg attagtccaa tttgttaaag acaggatatc agtggtccag gctctagttt
     6601 tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata aaataaaaga
     6661 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
     6721 tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt
     6781 tcagatcaag gtcaggaaca gatggaacag ggtcgaccct agagaaccat cagatgtttc
     6841 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
     6901 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
     6961 tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa
     7021 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
     7081 gtgattgact acccgtcagc gggggtcttt catttggggg ctcgtccggg atcgggagac
     7141 ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggctgcctc gcgcgtttcg
     7201 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     7261 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     7321 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtagatccgg ctgtggaatg
     7381 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7441 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7501 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7561 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7621 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7681 gcttttttgg aggcctaggc ttttgcaaaa agcttactgg cttaactatg cggcatcaga
     7741 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     7801 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7861 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7921 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7981 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8041 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8101 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8161 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8221 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8281 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8341 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8401 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     8461 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8521 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8581 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8641 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8701 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8761 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8821 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8881 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8941 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9001 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9061 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9121 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9181 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9241 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9301 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9361 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     9421 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9481 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9541 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9601 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9661 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9721 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     9781 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat tagcttggcc
     9841 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
     9901 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     9961 agttcatagc ccatatatgg agt
//
