LOCUS       sequence-392934-        9879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of 
            SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)] protease reporter 
            and P2A firefly luciferase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Arakawa M, Yoshida A, Okamura S, Ebina H, Morita E
  TITLE     A highly sensitive NanoLuc-based protease biosensor for detecting 
            apoptosis and SARS-CoV-2 infection.
  JOURNAL   Sci Rep. 2023 Jan 31;13(1):1753. doi: 10.1038/s41598-023-28984-4.
  PUBMED    36720982
REFERENCE   2  (bases 1 to 9879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-01-31"; volume: "13(1):1753. doi"; pages: " 
            10.1038/s41598-023-28984-4"
COMMENT     Sequence Label: 
            pQC.SARS2_5'UTR(SL1)-Flip-nluc(LgBiT1-8)CP[CoVA(Q5A)]-Fluc
FEATURES             Location/Qualifiers
     source          1..9879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        63..442
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        443..646
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             647..822
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    885..1084
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1285..1701
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1631..1653
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1669..1685
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1738..2041
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2042..2245
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2320..2329
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2710..2763
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2770..2779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3268..3315
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             3319..3438
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             3454..3510
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3517..5169
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    5273..5735
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5329..5346)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5556..5575
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5761..6360
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5761..5780)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6257..6277
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6719..7144
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7247..7269)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7405..7425)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7410..7739
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7590..7725
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7761..7780
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7861..7878)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8032..8620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8112..8131)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8791..9582,9583..9651))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9414..9433
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9652..9756)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9824..9842
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat taccgccatg
       61 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      121 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      181 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      241 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      301 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      361 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      421 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      481 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      541 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      601 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcaat aaaagagccc
      661 acaacccctc actcggcgcg ccagtcttcc gatagactgc gtcgcccggg tacccgtatt
      721 cccaataaag cctcttgctg tttgcatccg aatcgtggtc tcgctgttcc ttgggagggt
      781 ctcctctgag tgattgacta cccacgacgg gggtctttca tttgggggct cgtccgggat
      841 ttggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg gccagcaact
      901 tatctgtgtc tgtccgattg tctagtgtct atgtttgatg ttatgcgcct gcgtctgtac
      961 tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga gttctgaaca
     1021 cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg tggcccgacc
     1081 tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct ggtaggagac
     1141 gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg gaaccgaagc
     1201 cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg tctgactgtg
     1261 tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag tttgacctta
     1321 ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt caagaagaga
     1381 cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg gccgcgagac
     1441 ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc acctggcccg
     1501 catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc ttttgacccc
     1561 cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc tccatccgcc
     1621 ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct ttatccagcc
     1681 ctcactcctt ctctaggcgc cggaattgaa gatctggggg atcgatcctc tagagtccgt
     1741 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     1801 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     1861 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     1921 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     1981 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     2041 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     2101 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     2161 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     2221 gtgggaggtc tatataagca gagctcgttt agtgaaccga ttaaaggttt ataccttccc
     2281 aggtaacaaa ccaaccaggc ctcgtacgct taattaacgg ccaccatggg ctccctgctg
     2341 ttccgagtaa ccatcaacgg cggcggtgga ggctccgggg gaggcgggtc tggcggaggg
     2401 ggaagtggcg gtggtggctc tgaagttagc gctctggaaa aagaagtgtc tgcactcgag
     2461 aaagaagtaa gtgcccttga gaaggaggtg tccgcactcg agaaggaggt cagcgccctg
     2521 gaaaaggaaa aggtgagtgg gtggcgactt ttcaaaaaaa tctccggatc ctcagtggcc
     2581 gtgctggcta gcggcttcaa ggtgtccgcc ctgaaggaaa aagtaagcgc actgaaagaa
     2641 aaggtgagcg cgctgaagga gaaagtgagc gccctgaaag agaaagtctc tgcccttaag
     2701 gaggaattcg agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc
     2761 ccaaagcttg ccaccatggt tttcacattg gaagacttcg tgggtgactg ggagcagact
     2821 gccgcgtaca acttggatca agttctggag caaggtggag taagctccct cctgcaaaac
     2881 cttgcggtaa gcgttactcc catccaaagg atcgtaaggt ccggggaaaa tgctctcaaa
     2941 atagatatac acgttatcat accttacgaa gggctgtctg ccgatcagat ggctcaaata
     3001 gaagaagttt tcaaagtagt ctaccccgtg gacgatcatc attttaaggt gattctccct
     3061 tatgggaccc tcgttatcga cggggttaca cctaatatgc tcaattactt cggaaggcca
     3121 tatgaaggaa tagctgtttt cgatggtaaa aagattacgg tcaccggcac tctctggaat
     3181 gggaataaga taatagatga gagactgata acgccagacg gcgtggccgt gctggctagc
     3241 ggcttccaat tgggaggcag cgaagtggct tgcaagaact ggttcagtag cttaagccac
     3301 tttgtgatcc accttaattc tcacggcttt ccgcctgagg ttgaagagca agccgccggt
     3361 acattgccta tgtcctgcgc acaagaaagc ggtatggacc ggcacccagc cgcttgtgct
     3421 tcagctcgca tcaacgtcgg cggccgctct agagcaacca atttcagcct tcttaaacag
     3481 gctggagatg tggaagagaa tcctggacca gaattgatgg aagatgccaa aaacattaag
     3541 aagggcccag cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa
     3601 gccatgaagc gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag
     3661 gtggacatta cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag
     3721 cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc
     3781 ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc
     3841 tacaacgagc gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg
     3901 agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag
     3961 atcatcatca tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg
     4021 acttcccatt tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg
     4081 gacaaaacca tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta
     4141 gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc
     4201 aaccagatca tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc
     4261 atgttcacca cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc
     4321 gaggaggagc tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg
     4381 cccacactat ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac
     4441 ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc
     4501 aaacgcttcc acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc
     4561 attctgatca cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc
     4621 ttcgaggcta aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc
     4681 gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca
     4741 aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag
     4801 gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag
     4861 gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg
     4921 gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa
     4981 cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc
     5041 gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc
     5101 aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc
     5161 gccgtgtaac cgcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     5221 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     5281 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     5341 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     5401 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     5461 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     5521 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     5581 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     5641 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     5701 gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaggag acgaccttcc
     5761 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     5821 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     5881 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     5941 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6001 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6061 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6121 cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc accagggcaa gggtctgggc
     6181 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     6241 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     6301 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     6361 tgcttgcagg catctcatga gtgtgtcttc ccgttttccg cctgaggtca ctgcgtggat
     6421 ggagcgctgg cgcctgctgc gcgacggcga gctgctcacc acccactcga gggctgcagc
     6481 gctgcagagg ccgagtgcag aactgctcca aagggacctc aaggctttcc gagggacact
     6541 aggctgactc catcgagcca gtgtagagat aagcttatcg attagtccaa tttgttaaag
     6601 acaggatatc agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta
     6661 tagagtacga gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     6721 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
     6781 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag
     6841 ggtcgaccct agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct
     6901 gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc
     6961 cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact
     7021 gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt
     7081 ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt
     7141 catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg
     7201 ggaggtaagc tggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     7261 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     7321 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     7381 atagcggagt gtagatccgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     7441 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     7501 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7561 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7621 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7681 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     7741 agcttactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     7801 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     7861 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7921 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7981 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     8041 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8101 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8161 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8221 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8281 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8341 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8401 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8461 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8521 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8581 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8641 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8701 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8761 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8821 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8881 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8941 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9001 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9061 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
     9121 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9181 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9241 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9301 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9361 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
     9421 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9481 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9541 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9601 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9661 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9721 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9781 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     9841 gccctttcgt cttcaagaat tagcttggcc attgcatac
//
