LOCUS       sequence-392941-        9983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A] protease 
            reporter and P2A firefly luciferase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Arakawa M, Yoshida A, Okamura S, Ebina H, Morita E
  TITLE     A highly sensitive NanoLuc-based protease biosensor for detecting 
            apoptosis and SARS-CoV-2 infection.
  JOURNAL   Sci Rep. 2023 Jan 31;13(1):1753. doi: 10.1038/s41598-023-28984-4.
  PUBMED    36720982
REFERENCE   2  (bases 1 to 9983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-01-31"; volume: "13(1):1753. doi"; pages: " 
            10.1038/s41598-023-28984-4"
COMMENT     Sequence Label: pQC.Flip-nluc(LgBiT1-8)CP[Casp3A]-Fluc
FEATURES             Location/Qualifiers
     source          1..9983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        92..471
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        472..675
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             676..851
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    914..1113
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1314..1730
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1660..1682
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1698..1714
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1767..2070
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2071..2274
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2271..2295
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2442..2451
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2832..2885
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2892..2901
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3390..3437
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             3441..3560
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             3576..3632
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3639..5291
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    5406..5868
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5462..5479)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5689..5708
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5894..6493
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5894..5913)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6390..6410
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6852..7277
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7380..7402)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7538..7558)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7543..7872
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7723..7858
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7894..7913
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7994..8011)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8165..8753)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8245..8264)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8924..9715,9716..9784))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9547..9566
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9785..9889)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9957..9975
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat tagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat
       61 attggctcat gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag
      121 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      181 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      241 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      301 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      361 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      421 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      481 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      541 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      601 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      661 tatataagca gagctcaata aaagagccca caacccctca ctcggcgcgc cagtcttccg
      721 atagactgcg tcgcccgggt acccgtattc ccaataaagc ctcttgctgt ttgcatccga
      781 atcgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccacgacggg
      841 ggtctttcat ttgggggctc gtccgggatt tggagacccc tgcccaggga ccaccgaccc
      901 accaccggga ggtaagctgg ccagcaactt atctgtgtct gtccgattgt ctagtgtcta
      961 tgtttgatgt tatgcgcctg cgtctgtact agttagctaa ctagctctgt atctggcgga
     1021 cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg
     1081 actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc
     1141 cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
     1201 aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca
     1261 tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat tagggccaga
     1321 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc
     1381 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc
     1441 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg
     1501 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg
     1561 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
     1621 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
     1681 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattgaag
     1741 atctggggga tcgatcctct agagtccgtt acataactta cggtaaatgg cccgcctggc
     1801 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     1861 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     1921 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     1981 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2041 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2101 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2161 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2221 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     2281 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
     2341 cgggaccgat ccagcctccg cggccgggaa cggtgcattg gaacggacct gcaggaattg
     2401 atccgcggcc gcaccggtag gcctcgtacg cttaattaac ggccaccatg ggctccctgc
     2461 tgttccgagt aaccatcaac ggcggcggtg gaggctccgg gggaggcggg tctggcggag
     2521 ggggaagtgg cggtggtggc tctgaagtta gcgctctgga aaaagaagtg tctgcactcg
     2581 agaaagaagt aagtgccctt gagaaggagg tgtccgcact cgagaaggag gtcagcgccc
     2641 tggaaaagga aaaggtgagt gggtggcgac ttttcaaaaa aatctccgga tcctcaggag
     2701 atgaggtgga cggtggaagc aaggtgtccg ccctgaagga aaaagtaagc gcactgaaag
     2761 aaaaggtgag cgcgctgaag gagaaagtga gcgccctgaa agagaaagtc tctgccctta
     2821 aggaggaatt cgagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg
     2881 gcccaaagct tgccaccatg gttttcacat tggaagactt cgtgggtgac tgggagcaga
     2941 ctgccgcgta caacttggat caagttctgg agcaaggtgg agtaagctcc ctcctgcaaa
     3001 accttgcggt aagcgttact cccatccaaa ggatcgtaag gtccggggaa aatgctctca
     3061 aaatagatat acacgttatc ataccttacg aagggctgtc tgccgatcag atggctcaaa
     3121 tagaagaagt tttcaaagta gtctaccccg tggacgatca tcattttaag gtgattctcc
     3181 cttatgggac cctcgttatc gacggggtta cacctaatat gctcaattac ttcggaaggc
     3241 catatgaagg aatagctgtt ttcgatggta aaaagattac ggtcaccggc actctctgga
     3301 atgggaataa gataatagat gagagactga taacgccaga cggcggagat gaggtggacg
     3361 gtggaagcca attgggaggc agcgaagtgg cttgcaagaa ctggttcagt agcttaagcc
     3421 actttgtgat ccaccttaat tctcacggct ttccgcctga ggttgaagag caagccgccg
     3481 gtacattgcc tatgtcctgc gcacaagaaa gcggtatgga ccggcaccca gccgcttgtg
     3541 cttcagctcg catcaacgtc ggcggccgct ctagagcaac caatttcagc cttcttaaac
     3601 aggctggaga tgtggaagag aatcctggac cagaattgat ggaagatgcc aaaaacatta
     3661 agaagggccc agcgccattc tacccactcg aagacgggac cgccggcgag cagctgcaca
     3721 aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg
     3781 aggtggacat tacctacgcc gagtacttcg agatgagcgt tcggctggca gaagctatga
     3841 agcgctatgg gctgaataca aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt
     3901 tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc tgtggcccca gctaacgaca
     3961 tctacaacga gcgcgagctg ctgaacagca tgggcatcag ccagcccacc gtcgtattcg
     4021 tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa gaagctaccg atcatacaaa
     4081 agatcatcat catggatagc aagaccgact accagggctt ccaaagcatg tacaccttcg
     4141 tgacttccca tttgccaccc ggcttcaacg agtacgactt cgtgcccgag agcttcgacc
     4201 gggacaaaac catcgccctg atcatgaaca gtagtggcag taccggattg cccaagggcg
     4261 tagccctacc gcaccgcacc gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg
     4321 gcaaccagat catccccgac accgctatcc tcagcgtggt gccatttcac cacggcttcg
     4381 gcatgttcac cacgctgggc tacttgatct gcggctttcg ggtcgtgctc atgtaccgct
     4441 tcgaggagga gctattcttg cgcagcttgc aagactataa gattcaatct gccctgctgg
     4501 tgcccacact atttagcttc ttcgctaaga gcactctcat cgacaagtac gacctaagca
     4561 acttgcacga gatcgccagc ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg
     4621 ccaaacgctt ccacctacca ggcatccgcc agggctacgg cctgacagaa acaaccagcg
     4681 ccattctgat cacccccgaa ggggacgaca agcctggcgc agtaggcaag gtggtgccct
     4741 tcttcgaggc taaggtggtg gacttggaca ccggtaagac actgggtgtg aaccagcgcg
     4801 gcgagctgtg cgtccgtggc cccatgatca tgagcggcta cgttaacaac cccgaggcta
     4861 caaacgctct catcgacaag gacggctggc tgcacagcgg cgacatcgcc tactgggacg
     4921 aggacgagca cttcttcatc gtggaccggc tgaagagcct gatcaaatac aagggctacc
     4981 aggtagcccc agccgaactg gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg
     5041 gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg
     5101 aacacggtaa aaccatgacc gagaaggaga tcgtggacta tgtggccagc caggttacaa
     5161 ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg
     5221 gcaagttgga cgcccgcaag atccgcgaga ttctcattaa ggccaagaag ggcggcaaga
     5281 tcgccgtgta accgcccccc ctaacccccc ccccctaacg ttactggccg aagccgcttg
     5341 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     5401 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     5461 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     5521 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     5581 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     5641 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     5701 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     5761 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     5821 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaag
     5881 gagacgacct tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     5941 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     6001 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     6061 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     6121 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     6181 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     6241 ccggcccaag gagcccgcgt ggttcctggc caccgtcgga gtctcgcccg accaccaggg
     6301 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     6361 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     6421 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     6481 gcccggtgcc tgatgcttgc aggcatctca tgagtgtgtc ttcccgtttt ccgcctgagg
     6541 tcactgcgtg gatggagcgc tggcgcctgc tgcgcgacgg cgagctgctc accacccact
     6601 cgagggctgc agcgctgcag aggccgagtg cagaactgct ccaaagggac ctcaaggctt
     6661 tccgagggac actaggctga ctccatcgag ccagtgtaga gataagctta tcgattagtc
     6721 caatttgtta aagacaggat atcagtggtc caggctctag ttttgactca acaatatcac
     6781 cagctgaagc ctatagagta cgagccatag ataaaataaa agattttatt tagtctccag
     6841 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
     6901 ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc aaggtcagga
     6961 acagatggaa cagggtcgac cctagagaac catcagatgt ttccagggtg ccccaaggac
     7021 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     7081 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt
     7141 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     7201 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     7261 agcgggggtc tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac
     7321 cgacccacca ccgggaggta agctggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     7381 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     7441 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     7501 cagtcacgta gcgatagcgg agtgtagatc cggctgtgga atgtgtgtca gttagggtgt
     7561 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7621 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7681 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     7741 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7801 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7861 ggcttttgca aaaagcttac tggcttaact atgcggcatc agagcagatt gtactgagag
     7921 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     7981 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8041 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8101 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8161 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8221 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8281 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8341 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8401 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8461 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8521 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8581 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     8641 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8701 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8761 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8821 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8881 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8941 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     9001 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9061 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9121 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9181 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     9241 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9301 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9361 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9421 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9481 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9541 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9601 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9661 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9721 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9781 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9841 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9901 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     9961 ggcgtatcac gaggcccttt cgt
//
