LOCUS       sequence-392943-        9983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)] protease
            reporter and P2A firefly luciferase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Arakawa M, Yoshida A, Okamura S, Ebina H, Morita E
  TITLE     A highly sensitive NanoLuc-based protease biosensor for detecting 
            apoptosis and SARS-CoV-2 infection.
  JOURNAL   Sci Rep. 2023 Jan 31;13(1):1753. doi: 10.1038/s41598-023-28984-4.
  PUBMED    36720982
REFERENCE   2  (bases 1 to 9983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-01-31"; volume: "13(1):1753. doi"; pages: " 
            10.1038/s41598-023-28984-4"
COMMENT     Sequence Label: pQC.Flip-nluc(LgBiT1-8)CP[Casp3A(D5A)]-Fluc
FEATURES             Location/Qualifiers
     source          1..9983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        73..452
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        453..656
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             657..832
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    895..1094
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1295..1711
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1641..1663
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1679..1695
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1748..2051
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2052..2255
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2252..2276
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2423..2432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2813..2866
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2873..2882
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3371..3418
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             3422..3541
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             3557..3613
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3620..5272
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    5387..5849
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5443..5460)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5670..5689
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5875..6474
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5875..5894)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6371..6391
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6833..7258
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7361..7383)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7519..7539)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7524..7853
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7704..7839
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7875..7894
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7975..7992)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8146..8734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8226..8245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8905..9696,9697..9765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9528..9547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9766..9870)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9938..9956
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat
       61 taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      121 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      181 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      241 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      301 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      361 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      421 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      481 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      541 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      601 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcaat
      661 aaaagagccc acaacccctc actcggcgcg ccagtcttcc gatagactgc gtcgcccggg
      721 tacccgtatt cccaataaag cctcttgctg tttgcatccg aatcgtggtc tcgctgttcc
      781 ttgggagggt ctcctctgag tgattgacta cccacgacgg gggtctttca tttgggggct
      841 cgtccgggat ttggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      901 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgtttgatg ttatgcgcct
      961 gcgtctgtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
     1021 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
     1081 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
     1141 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
     1201 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
     1261 tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag
     1321 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1381 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1441 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1501 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1561 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1621 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1681 ttatccagcc ctcactcctt ctctaggcgc cggaattgaa gatctggggg atcgatcctc
     1741 tagagtccgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     1801 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     1861 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     1921 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     1981 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     2041 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     2101 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     2161 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     2221 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     2281 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
     2341 gcggccggga acggtgcatt ggaacggacc tgcaggaatt gatccgcggc cgcaccggta
     2401 ggcctcgtac gcttaattaa cggccaccat gggctccctg ctgttccgag taaccatcaa
     2461 cggcggcggt ggaggctccg ggggaggcgg gtctggcgga gggggaagtg gcggtggtgg
     2521 ctctgaagtt agcgctctgg aaaaagaagt gtctgcactc gagaaagaag taagtgccct
     2581 tgagaaggag gtgtccgcac tcgagaagga ggtcagcgcc ctggaaaagg aaaaggtgag
     2641 tgggtggcga cttttcaaaa aaatctccgg atcctcagga gatgaggtgg ccggtggaag
     2701 caaggtgtcc gccctgaagg aaaaagtaag cgcactgaaa gaaaaggtga gcgcgctgaa
     2761 ggagaaagtg agcgccctga aagagaaagt ctctgccctt aaggaggaat tcgagggcag
     2821 aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccaaagc ttgccaccat
     2881 ggttttcaca ttggaagact tcgtgggtga ctgggagcag actgccgcgt acaacttgga
     2941 tcaagttctg gagcaaggtg gagtaagctc cctcctgcaa aaccttgcgg taagcgttac
     3001 tcccatccaa aggatcgtaa ggtccgggga aaatgctctc aaaatagata tacacgttat
     3061 cataccttac gaagggctgt ctgccgatca gatggctcaa atagaagaag ttttcaaagt
     3121 agtctacccc gtggacgatc atcattttaa ggtgattctc ccttatggga ccctcgttat
     3181 cgacggggtt acacctaata tgctcaatta cttcggaagg ccatatgaag gaatagctgt
     3241 tttcgatggt aaaaagatta cggtcaccgg cactctctgg aatgggaata agataataga
     3301 tgagagactg ataacgccag acggcggaga tgaggtggcc ggtggaagcc aattgggagg
     3361 cagcgaagtg gcttgcaaga actggttcag tagcttaagc cactttgtga tccaccttaa
     3421 ttctcacggc tttccgcctg aggttgaaga gcaagccgcc ggtacattgc ctatgtcctg
     3481 cgcacaagaa agcggtatgg accggcaccc agccgcttgt gcttcagctc gcatcaacgt
     3541 cggcggccgc tctagagcaa ccaatttcag ccttcttaaa caggctggag atgtggaaga
     3601 gaatcctgga ccagaattga tggaagatgc caaaaacatt aagaagggcc cagcgccatt
     3661 ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga agcgctacgc
     3721 cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca ttacctacgc
     3781 cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg ggctgaatac
     3841 aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg
     3901 tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg agcgcgagct
     3961 gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga aagggctgca
     4021 aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca tcatggatag
     4081 caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc atttgccacc
     4141 cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct
     4201 gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac cgcaccgcac
     4261 cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga tcatccccga
     4321 caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca ccacgctggg
     4381 ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg agctattctt
     4441 gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac tatttagctt
     4501 cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg agatcgccag
     4561 cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct tccacctacc
     4621 aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga tcacccccga
     4681 aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg ctaaggtggt
     4741 ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt gcgtccgtgg
     4801 ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc tcatcgacaa
     4861 ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc acttcttcat
     4921 cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc cagccgaact
     4981 ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg gcctgcccga
     5041 cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta aaaccatgac
     5101 cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga agctgcgcgg
     5161 tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg acgcccgcaa
     5221 gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtgt aaccgccccc
     5281 ccccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     5341 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     5401 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     5461 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     5521 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     5581 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     5641 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     5701 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     5761 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     5821 tggttttcct ttgaaaaaca cgatgataag cttgccacaa ggagacgacc ttccatgacc
     5881 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     5941 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6001 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6061 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6121 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6181 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6241 tggttcctgg ccaccgtcgg agtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6301 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6361 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6421 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatgcttg
     6481 caggcatctc atgagtgtgt cttcccgttt tccgcctgag gtcactgcgt ggatggagcg
     6541 ctggcgcctg ctgcgcgacg gcgagctgct caccacccac tcgagggctg cagcgctgca
     6601 gaggccgagt gcagaactgc tccaaaggga cctcaaggct ttccgaggga cactaggctg
     6661 actccatcga gccagtgtag agataagctt atcgattagt ccaatttgtt aaagacagga
     6721 tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt
     6781 acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     6841 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     6901 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagggtcga
     6961 ccctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
     7021 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     7081 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
     7141 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
     7201 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg
     7261 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt
     7321 aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     7381 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     7441 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     7501 gagtgtagat ccggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     7561 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     7621 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     7681 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     7741 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     7801 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta
     7861 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     7921 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     7981 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8041 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8101 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8161 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8221 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8281 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8341 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8401 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8461 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8521 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8581 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8641 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8701 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8761 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8821 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8881 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8941 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9001 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9061 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9121 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9181 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     9241 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9301 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9361 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9421 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9481 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     9541 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9601 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9661 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9721 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9781 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9841 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9901 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     9961 tcgtcttcaa gaattagctt ggc
//
