LOCUS       sequence-392959-        2491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid contains the coding sequence for signaling peptide M6, 
            which contains the ITYAAV signaling motif. Digest this insert and 
            add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Daniels KG, Wang S, Simic MS, Bhargava HK, Capponi S, Tonai Y, Yu W,
            Bianco S, Lim WA
  TITLE     Decoding CAR T cell phenotype using combinatorial signaling motif 
            libraries and machine learning.
  JOURNAL   Science. 2022 Dec 16;378(6625):1194-1200. doi: 
            10.1126/science.abq0225. Epub 2022 Dec 8.
  PUBMED    36480602
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abq0225"; journalName: "Science"; date: 
            "2022-12-16- 16"; volume: "378"; issue: "6625"; pages: "1194-1200"
COMMENT     Sequence Label: pMAT_M6_ITYAAV
FEATURES             Location/Qualifiers
     source          1..2491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(634..651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(744..1332)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(824..843)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1503..2294,2295..2363))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2126..2145
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2364..2468)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccgcatc ggtacccggg gatccacctg caacactccg gatcaggatc taagcctatg
      421 gccgagagca tcacatatgc cgccgtggcc agacattctg ccggaggctc cggatcagga
      481 tccggcagtg gtacaaggca ggtggatcct ctagagtcgc tgggcctcat gggccttccg
      541 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaac atggtcatag
      601 ctgtttcctt gcgtattggg cgctctccgc ttcctcgctc actgactcgc tgcgctcggt
      661 cgttcgggta aagcctgggg tgcctaatga gcaaaaggcc agcaaaaggc caggaaccgt
      721 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      781 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      841 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      901 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
      961 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1021 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1081 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1141 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     1201 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     1261 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     1321 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     1381 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     1441 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     1501 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     1561 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     1621 cccagtgctg caatgatacc gcgagaacca cgctcaccgg ctccagattt atcagcaata
     1681 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     1741 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     1801 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     1861 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     1921 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     1981 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2041 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2101 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     2161 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     2221 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     2281 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     2341 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     2401 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     2461 gttccgcgca catttccccg aaaagtgcca c
//
