LOCUS       sequence-392966-        8414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone (attL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Sretenovic S, Green Y, Wu Y, Cheng Y, Zhang T, Van Eck J, Qi Y
  TITLE     Genome- and transcriptome-wide off-target analyses of a 
            high-efficiency adenine base editor in tomato.
  JOURNAL   Plant Physiol. 2023 Aug 31;193(1):291-303. doi: 
            10.1093/plphys/kiad347.
  PUBMED    37315207
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Plant Physiol"; date: "2023-08-31- 31"; volume: "193"; issue: "1"; 
            pages: "291-303"
COMMENT     Sequence Label: pYPQ262F-ABE8e
FEATURES             Location/Qualifiers
     source          1..8414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             390..1181
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1274..1862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1763..1782
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2016..2033
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2192..2219)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2311..2397)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2446..2468
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2460..2477
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2461..2477
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2493..2592
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2632..2652
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3253..3318
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3325..3345
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3370..3393,3394..3396,3397..7470)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             7383..7391
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7471..7518
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     protein_bind    8193..8316
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8333..8352)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8334..8352)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8357..8373)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8357..8373)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cacaagataa aaatatatca tcatgcctcc tctggaccag ccaggacaga aatgcctcga
       61 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
      121 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
      181 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
      241 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
      301 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
      361 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
      421 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
      481 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
      541 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
      601 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
      661 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
      721 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
      781 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
      841 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
      901 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
      961 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     1021 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     1081 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     1141 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac
     1201 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     1261 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1321 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1381 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1441 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1501 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1561 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1621 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1681 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1741 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1801 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     1861 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1921 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1981 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2041 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2101 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta
     2161 ccgcgagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg
     2221 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg
     2281 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga
     2341 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg
     2401 cctggcagtt ccctactctc gcgttaacgc ttgcatggat gttttcccag tcacgacgtt
     2461 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
     2521 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
     2581 aaaaagcagg ctccgatgaa acggacagcc gacggaagcg agttcgagtc accaaagaag
     2641 aagcggaaag tctctgaggt ggagttttcc cacgagtact ggatgagaca tgccctgacc
     2701 ctggccaaga gggcacggga tgagagggag gtgcctgtgg gagccgtgct ggtgctgaac
     2761 aatagagtga tcggcgaggg ctggaacaga gccatcggcc tgcacgaccc aacagcccat
     2821 gccgaaatta tggccctgag acagggcggc ctggtcatgc agaactacag actgattgac
     2881 gccaccctgt acgtgacatt cgagccttgc gtgatgtgcg ccggcgccat gatccactct
     2941 aggatcggcc gcgtggtgtt tggctggagg aactcaaaaa gaggcgccgc aggctccctg
     3001 atgaacgtgc tgaactaccc cggcatgaat caccgcgtcg aaattaccga gggaatcctg
     3061 gcagatgaat gtgccgccct gctgtgcgat ttctatcgga tgcctagaca ggtgttcaat
     3121 gctcagaaga aggcccagag ctccatcaac tccggaggat ctagcggagg ctcctctggc
     3181 tctgagacac ctggcacaag cgagagcgca acacctgaaa gcagcggggg cagcagcggg
     3241 gggtcaacca tggattacaa ggaccacgac ggggattaca aggaccacga cattgattac
     3301 aaggatgatg atgacaagat ggctccgaag aagaagagga aggttggcat ccacggggtg
     3361 ccagctgctg acaagaagta ctcgatcggc ctcgctattg ggactaactc tgttggctgg
     3421 gccgtgatca ccgacgagta caaggtgccc tcaaagaagt tcaaggtcct gggcaacacc
     3481 gatcggcatt ccatcaagaa gaatctcatt ggcgctctcc tgttcgacag cggcgagacg
     3541 gctgaggcta cgcggctcaa gcgcaccgcc cgcaggcggt acacgcgcag gaagaatcgc
     3601 atctgctacc tgcaggagat tttctccaac gagatggcga aggttgacga ttctttcttc
     3661 cacaggctgg aggagtcatt cctcgtggag gaggataaga agcacgagcg gcatccaatc
     3721 ttcggcaaca ttgtcgacga ggttgcctac cacgagaagt accctacgat ctaccatctg
     3781 cggaagaagc tcgtggactc cacagataag gcggacctcc gcctgatcta cctcgctctg
     3841 gcccacatga ttaagttcag gggccatttc ctgatcgagg gggatctcaa cccggacaat
     3901 agcgatgttg acaagctgtt catccagctc gtgcagacgt acaaccagct cttcgaggag
     3961 aaccccatta atgcgtcagg cgtcgacgcg aaggctatcc tgtccgctag gctctcgaag
     4021 tctcggcgcc tcgagaacct gatcgcccag ctgccgggcg agaagaagaa cggcctgttc
     4081 gggaatctca ttgcgctcag cctggggctc acgcccaact tcaagtcgaa tttcgatctc
     4141 gctgaggacg ccaagctgca gctctccaag gacacatacg acgatgacct ggataacctc
     4201 ctggcccaga tcggcgatca gtacgcggac ctgttcctcg ctgccaagaa tctgtcggac
     4261 gccatcctcc tgtctgatat tctcagggtg aacaccgaga ttacgaaggc tccgctctca
     4321 gcctccatga tcaagcgcta cgacgagcac catcaggatc tgaccctcct gaaggcgctg
     4381 gtcaggcagc agctccccga gaagtacaag gagatcttct tcgatcagtc gaagaacggc
     4441 tacgctgggt acattgacgg cggggcctct caggaggagt tctacaagtt catcaagccg
     4501 attctggaga agatggacgg cacggaggag ctgctggtga agctcaatcg cgaggacctc
     4561 ctgaggaagc agcggacatt cgataacggc agcatcccac accagattca tctcggggag
     4621 ctgcacgcta tcctgaggag gcaggaggac ttctaccctt tcctcaagga taaccgcgag
     4681 aagatcgaga agattctgac tttcaggatc ccgtactacg tcggcccact cgctaggggc
     4741 aactcccgct tcgcttggat gacccgcaag tcagaggaga cgatcacgcc gtggaacttc
     4801 gaggaggtgg tcgacaaggg cgctagcgct cagtcgttca tcgagaggat gacgaatttc
     4861 gacaagaacc tgccaaatga gaaggtgctc cctaagcact cgctcctgta cgagtacttc
     4921 acagtctaca acgagctgac taaggtgaag tatgtgaccg agggcatgag gaagccggct
     4981 ttcctgtctg gggagcagaa gaaggccatc gtggacctcc tgttcaagac caaccggaag
     5041 gtcacggtta agcagctcaa ggaggactac ttcaagaaga ttgagtgctt cgattcggtc
     5101 gagatctctg gcgttgagga ccgcttcaac gcctccctgg ggacctacca cgatctcctg
     5161 aagatcatta aggataagga cttcctggac aacgaggaga atgaggatat cctcgaggac
     5221 attgtgctga cactcactct gttcgaggac cgggagatga tcgaggagcg cctgaagact
     5281 tacgcccatc tcttcgatga caaggtcatg aagcagctca agaggaggag gtacaccggc
     5341 tgggggaggc tgagcaggaa gctcatcaac ggcattcggg acaagcagtc cgggaagacg
     5401 atcctcgact tcctgaagag cgatggcttc gcgaaccgca atttcatgca gctgattcac
     5461 gatgacagcc tcacattcaa ggaggatatc cagaaggctc aggtgagcgg ccagggggac
     5521 tcgctgcacg agcatatcgc gaacctcgct ggctcgccag ctatcaagaa ggggattctg
     5581 cagaccgtga aggttgtgga cgagctggtg aaggtcatgg gcaggcacaa gcctgagaac
     5641 atcgtcattg agatggcccg ggagaatcag accacgcaga agggccagaa gaactcacgc
     5701 gagaggatga agaggatcga ggagggcatt aaggagctgg ggtcccagat cctcaaggag
     5761 cacccggtgg agaacacgca gctgcagaat gagaagctct acctgtacta cctccagaat
     5821 ggccgcgata tgtatgtgga ccaggagctg gatattaaca ggctcagcga ttacgacgtc
     5881 gatcatatcg ttccacagtc attcctgaag gatgactcca ttgacaacaa ggtcctcacc
     5941 aggtcggaca agaaccgggg caagtctgat aatgttcctt cagaggaggt cgttaagaag
     6001 atgaagaact actggcgcca gctcctgaat gccaagctga tcacgcagcg gaagttcgat
     6061 aacctcacaa aggctgagag gggcgggctc tctgagctgg acaaggcggg cttcatcaag
     6121 aggcagctgg tcgagacacg gcagatcact aagcacgttg cgcagattct cgactcacgg
     6181 atgaacacta agtacgatga gaatgacaag ctgatccgcg aggtgaaggt catcaccctg
     6241 aagtcaaagc tcgtctccga cttcaggaag gatttccagt tctacaaggt tcgggagatc
     6301 aacaattacc accatgccca tgacgcgtac ctgaacgcgg tggtcggcac agctctgatc
     6361 aagaagtacc caaagctcga gagcgagttc gtgtacgggg actacaaggt ttacgatgtg
     6421 aggaagatga tcgccaagtc ggagcaggag attggcaagg ctaccgccaa gtacttcttc
     6481 tactctaaca ttatgaattt cttcaagaca gagatcactc tggccaatgg cgagatccgg
     6541 aagcgccccc tcatcgagac gaacggcgag acgggggaga tcgtgtggga caagggcagg
     6601 gatttcgcga ccgtcaggaa ggttctctcc atgccacaag tgaatatcgt caagaagaca
     6661 gaggtccaga ctggcgggtt ctctaaggag tcaattctgc ctaagcggaa cagcgacaag
     6721 ctcatcgccc gcaagaagga ctgggatccg aagaagtacg gcgggttcga cagccccact
     6781 gtggcctact cggtcctggt tgtggcgaag gttgagaagg gcaagtccaa gaagctcaag
     6841 agcgtgaagg agctgctggg gatcacgatt atggagcgct ccagcttcga gaagaacccg
     6901 atcgatttcc tggaggcgaa gggctacaag gaggtgaaga aggacctgat cattaagctc
     6961 cccaagtact cactcttcga gctggagaac ggcaggaagc ggatgctggc ttccgctggc
     7021 gagctgcaga aggggaacga gctggctctg ccgtccaagt atgtgaactt cctctacctg
     7081 gcctcccact acgagaagct caagggcagc cccgaggaca acgagcagaa gcagctgttc
     7141 gtcgagcagc acaagcatta cctcgacgag atcattgagc agatttccga gttctccaag
     7201 cgcgtgatcc tggccgacgc gaatctggat aaggtcctct ccgcgtacaa caagcaccgc
     7261 gacaagccaa tcagggagca ggctgagaat atcattcatc tcttcaccct gacgaacctc
     7321 ggcgcccctg ctgctttcaa gtacttcgac acaactatcg atcgcaagag gtacacaagc
     7381 actaaggagg tcctggacgc gaccctcatc caccagtcga ttaccggcct ctacgagacg
     7441 cgcatcgacc tgtctcagct cgggggcgac aagcggccag cggcgacgaa gaaggcgggg
     7501 caggcgaaga agaagaagtg agctcagagc tttcgttcgt atcatcggtt tcgacaacgt
     7561 tcgtcaagtt caatgcatca gtttcattgc gcacacacca gaatcctact gagtttgagt
     7621 attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg
     7681 ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg
     7741 aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt
     7801 tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg
     7861 tcaaatcgtg gcctctaatg accgaagtta atatgaggag taaaacactt gtagttgtac
     7921 cattatgctt attcactagg caacaaatat attttcagac ctagaaaagc tgcaaatgtt
     7981 actgaataca agtatgtcct cttgtgtttt agacatttat gaactttcct ttatgtaatt
     8041 ttccagaatc cttgtcagat tctaatcatt gctttataat tatagttata ctcatggatt
     8101 tgtagttgag tatgaaaata ttttttaatg cattttatga cttgccaatt gttccggaac
     8161 tctagataag cttaccggaa agggcgaatt cgcaactttg tatacaaaag ttgaacgaga
     8221 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     8281 ataatactgt aaaacacaac atatccagtc actatgccat ccagctgata tcccctatag
     8341 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     8401 ctgatgttac attg
//
