LOCUS       sequence-392970-        8502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of truncated HSF2 (DBD and HR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Price RM, Budzynski MA, Shen J, Mitchell JE, Kwan JZJ, Teves SS
  TITLE     Heat shock transcription factors demonstrate a distinct mode of 
            interaction with mitotic chromosomes.
  JOURNAL   Nucleic Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304.
  PUBMED    37114996
REFERENCE   2  (bases 1 to 8502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304."
COMMENT     Sequence Label: Halo-H2DBD-HR
FEATURES             Location/Qualifiers
     source          1..8502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     98..117
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(336..440)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(466..921)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(603..624)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     815..834
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1048..1070
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1062..1079
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1063..1079
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1209..1271
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1724..1935
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1948..2216
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2242..2251
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2251..2316
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2326..3216
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             3229..3249
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     misc_feature    3893..4464
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4059..4076)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4286..4305
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4473..5267
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4527..4546)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5137..5156
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5345..5365)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    5407..5528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5444..5463)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5498..5517
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6291..6325
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6816..6832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6816..6832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6829..6851)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6840..6856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6864..6870,6871..6888,6889..6894))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6909..6930
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7047..7064)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7218..7806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7298..7317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7977..8502,1..266,267..335))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
       61 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
      121 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
      181 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
      241 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
      301 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
      361 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
      421 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
      481 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
      541 ccgaaatcgg caaaacccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
      601 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgcc aaagggcgaa
      661 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
      721 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
      781 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
      841 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
      901 atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc
      961 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     1021 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     1081 agcgcgcctc gttcattcac gtttttgaac ccgtggagga cgggcagact cgcggtgcaa
     1141 atgtgtttta cagcgtgatg gagcagatga agatgctcgg cacgctgcag aacacgcagc
     1201 tagattaacc ctagaaagat aatcatattg tgacgtacgt taaagataat catgtgtaaa
     1261 attgacgcat gtgttttatc ggtctgtata tcgaggttta tttattaatt tgaatagata
     1321 ttaagtttta ttatatttac acttacatac taataataaa ttcaacaaac aatttattta
     1381 tgtttattta tttattaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac
     1441 ttttatgagg gacagccctc ccccaaagcc cccagggatg taattacgtc cctcccccgc
     1501 tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc cccgcatccc
     1561 cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc
     1621 tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac ggggaaaagg
     1681 cctccacggc caaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     1741 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     1801 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     1861 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     1921 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     1981 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     2041 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     2101 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     2161 ctgcttgccc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     2221 ctgtgaccgg cgcctactct agccaccatg gactacaaag accatgacgg tgattataaa
     2281 gatcatgaca tcgattacaa ggatgacgat gacaagcttg gctccatggc agaaatcggt
     2341 actggctttc cattcgaccc ccattatgtg gaagtcctgg gcgagcgcat gcactacgtc
     2401 gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc acggtaaccc gacctcctcc
     2461 tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc atcgctgcat tgctccagac
     2521 ctgatcggta tgggcaaatc cgacaaacca gacctgggtt atttcttcga cgaccacgtc
     2581 cgcttcatgg atgccttcat cgaagccctg ggtctggaag aggtcgtcct ggtcattcac
     2641 gactggggct ccgctctggg tttccactgg gccaagcgca atccagagcg cgtcaaaggt
     2701 attgcattta tggagttcat ccgccctatc ccgacctggg acgaatggcc agaatttgcc
     2761 cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc gcaagctgat catcgatcag
     2821 aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc gcccgctgac tgaagtcgag
     2881 atggaccatt accgcgagcc gttcctgaat cctgttgacc gcgagccact gtggcgcttc
     2941 ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg tcgcgctggt cgaagaatac
     3001 atggactggc tgcaccagtc ccctgtcccg aagctgctgt tctggggcac cccaggcgtt
     3061 ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc tgcctaactg caaggctgtg
     3121 gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc cggacctgat cggcagcgag
     3181 atcgcgcgct ggctgtcgac gctcgagatt tccggcgagc caaccactga ggatctgtac
     3241 tttcagagcg ataactctag aatgaagcag agttccaacg tgccggcttt cctcagcaag
     3301 ctgtggacgc ttgtggagga aacccacacc aacgagttca tcacctggag tcagaatgga
     3361 caaagttttc tggtcttgga tgagcaaaga tttgcaaagg aaattcttcc taagtacttc
     3421 aaacacaata acatggcgag ctttgtgaga caactaaata tgtatggctt ccgaaaagta
     3481 gtgcatatcg aatctggaat tatcaaacag gaaagagatg gccctgttga atttcagcat
     3541 ccttatttca agcaaggcca ggatgacctg ttggagaaca ttaaaaggaa ggtttcatct
     3601 tcaaaaccag aggaaaataa aattcgtcag gaagatttaa caaaaattat tagtagtgct
     3661 cagaaggttc aaataaaaca agaaactatt gagtccaggc tttcagaatt aaaaagtgag
     3721 aatgaatccc tttggaagga ggtgtcagaa ctaagagcaa agcatgccca gcagcaacaa
     3781 gttattcgga agattgtcca gtttattgtt acattggttc agaataatca acttgtgagt
     3841 ttaaaacgta aaaggcctct acttctaaac acaaatggag ccccaaagta agcccctctc
     3901 cctccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     3961 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     4021 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     4081 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     4141 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc cctgcggcca
     4201 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     4261 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     4321 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     4381 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     4441 tttcctttga aaaacacgat aatacctccg gaatgattga acaagatgga ttgcacgcag
     4501 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     4561 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     4621 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     4681 tggccgcgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     4741 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     4801 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     4861 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     4921 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     4981 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     5041 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     5101 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     5161 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     5221 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagtc gacaatcaac
     5281 ctctggtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5341 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcgt
     5401 taactaaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5461 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5521 gtatcttatc atgtctggaa ttgactcaaa tgatgtcaat tagtctatca gaagctcatc
     5581 tggtctccct tccgggggac aagacatccc tgtttaatat ttaaacagca gtgttcccaa
     5641 actgggttct tatatccctt gctctggtca accaggttgc agggtttcct gtcctcacag
     5701 gaacgaagtc cctaaagaaa cagtggcagc caggtttagc cccggaatta actggattcc
     5761 ttttttaggg cccattggta tggctttttc cccgtatccc cccaggtgtc tgcaggctca
     5821 aagagcagcg agaagcgttc agaggaaagc gatcccgtgc cgccttcccc gtgcccgggc
     5881 tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc
     5941 gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg
     6001 ggggggggct gtccctgata tctataacaa gaaaatatat atatgataag ttatcacgta
     6061 agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa
     6121 agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta
     6181 ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca
     6241 cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa
     6301 ttttacgcag actatctttc tagggttaat ctagctgcat caggatcata tcgtcgggtc
     6361 ttttttccgg ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg aggaagaagc
     6421 ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag tttgccgagg atgactgctg
     6481 ctgcattgac gttgagcgaa aacgcacgtt taccatgatg attcgggaag gtgtggccat
     6541 gcacgccttt aacggtgaac tgttcgttca ggccacctgg gataccagtt cgtcgcggct
     6601 tttccggaca cagttccgga tggtcagccc gaagcgcatc agcaacccga acaataccgg
     6661 cgacagccgg aactgccgtg ccggtgtgca gattaatgac agcggtgcgg cgctgggata
     6721 ttacgtcagc gaggacgggt atcctggctg gatgccgcag aaatggacat ggataccccg
     6781 tgagttaccc ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6841 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6901 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6961 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7021 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7081 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7141 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7201 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7261 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7321 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7381 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7441 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7501 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7561 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7621 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7681 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7741 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7801 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7861 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7921 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7981 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8041 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8101 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8161 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8221 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8281 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8341 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8401 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8461 gttatggcag cactgcataa ttctcttact gtcatgccat cc
//
