LOCUS       sequence-392976-        9488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of full length HSF2 with SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9488)
  AUTHORS   Price RM, Budzynski MA, Shen J, Mitchell JE, Kwan JZJ, Teves SS
  TITLE     Heat shock transcription factors demonstrate a distinct mode of 
            interaction with mitotic chromosomes.
  JOURNAL   Nucleic Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304.
  PUBMED    37114996
REFERENCE   2  (bases 1 to 9488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304."
COMMENT     Sequence Label: NLS-Halo-HSF2
FEATURES             Location/Qualifiers
     source          1..9488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4..23)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(683..1474,1475..1543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1306..1325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1544..1648)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1674..2129)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1811..1832)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2023..2042
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2270..2287
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2271..2287
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2417..2479
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2933..3144
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3157..3425
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3451..3460
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3460..3525
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3529..3549
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3553..3573
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3577..3597
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3604..4494
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNRVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             4507..4527
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     misc_feature    6103..6675
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6270..6287)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6497..6516
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6684..7478
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6738..6757)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7348..7367
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7538..7558)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    7600..7721
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7637..7656)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7691..7710
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8485..8519
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9010..9026)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9010..9026)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9023..9045)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9034..9050
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9058..9064,9065..9082,9083..9088))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9103..9124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9241..9258)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9412..9488,1..512))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
       61 tgccgcttac cggatacctg tccgcctttc tcccttcggg gagcgtggcg ctttctcata
      121 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      181 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      241 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      301 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      361 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
      421 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
      481 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
      541 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
      601 ggatcttcgc ctagatcctt ttaaattaaa aatgaagtct taaatcaatc taaagtatat
      661 atgagtaaac ttggtctgac agttaccaat gtttaatcag tgaggcacct atctcagcga
      721 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
      781 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
      841 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
      901 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
      961 cgccggttaa tagtttacgc aacgttgttg ccattgctac aggcatcgtg gtgtcgcgct
     1021 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     1081 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     1141 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     1201 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     1261 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     1321 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     1381 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     1441 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     1501 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     1561 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     1621 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     1681 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     1741 ccaataggcc gaaatcggca aaatccctta taaatcaaaa ggatagaccg agatagggtt
     1801 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     1861 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     1921 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     1981 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     2041 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     2101 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     2161 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     2221 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacatt gtaaaacgac
     2281 ggccagtgag cgcgcctcgt tcattcacgt ttttgaaccc gtggaggacg ggcagactcg
     2341 cggtgcaaat gtgttttaca gcgtgatgga gcagatgaag atgctcgaca cgctgcagaa
     2401 cacgcagcta gattaaccct agaaagataa tcatattgtg acgtacgtta aagataatca
     2461 tgtgtaaaat tgacgcatgt gttttatcgg tctgtatatc gaggtttatt tattaatttg
     2521 aatagatatt aagttttatt atatttacac ttacatacta ataataaatt caacaaacaa
     2581 tttatttatg tttatttatt tattaaaaaa aacaaaaact caaaatttct tctataaagt
     2641 aacaaaactt ttatgaggga cagccccccc cccaaagccc ccagggatgt aattacgtcc
     2701 ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc
     2761 ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga
     2821 tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg
     2881 gggaaaaggc ctccacggcc aaggatctgc gatcgctccg gtgcccgcca gtgggcagag
     2941 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     3001 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     3061 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     3121 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     3181 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     3241 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     3301 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     3361 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     3421 agatccaagc tgtgaccggc gcctactcta gccaccatgg actacaaaga ccatgacggt
     3481 gattataaag atcatgacat cgattacaag gatgacgatg acaagcttcc aaaaaagaag
     3541 agaaaggtag atcccaagaa aaagagaaag gtcgatccaa agaagaaaag aaaagtgggc
     3601 tccatggcag aaatcggtac tggctttcca ttcgaccccc attatgtgga agtcctgggc
     3661 gagcgcatgc actacgtcga tgttggtccg cgcgatggca cccctgtgct gttcctgcac
     3721 ggtaacccga cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc accgacccat
     3781 cgctgcattg ctccagacct gatcggtatg ggcaaatccg acaaaccaga cctgggttat
     3841 ttcttcgacg accacgtccg cttcatggat gccttcatcg aagccctggg tctggaagag
     3901 gtcgtcctgg tcattcacga ctggggctcc gctctgggtt tccactgggc caagcgcaat
     3961 ccagagcgcg tcaaaggtat tgcatttatg gagttcatcc gccctatccc gacctgggac
     4021 gaatggccag aatttgcccg cgagaccttc caggccttcc gcaccaccga cgtcggccgc
     4081 aagctgatca tcgatcagaa cgtttttatc gagggtacgc tgccgatggg tgtcgtccgc
     4141 ccgctgactg aagtcgagat ggaccattac cgcgagccgt tcctgaatcg tgttgaccgc
     4201 gagccactgt ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc gaacatcgtc
     4261 gcgctggtcg aagaatacat ggactggctg caccagtccc ctgtcccgaa gctgctgttc
     4321 tggggcaccc caggcgttct gatcccaccg gccgaagccg ctcgcctggc caaaagcctg
     4381 cctaactgca aggctgtgga catcggcccg ggtctgaatc tgctgcaaga agacaacccg
     4441 gacctgatcg gcagcgagat cgcgcgctgg ctgtcgacgc tcgagatttc cggcgagcca
     4501 accactgagg atctgtactt tcagagcgat aactctagaa tgaagcagag ttccaacgtg
     4561 ccggctttcc tcagcaagct gtggacgctt gtggaggaaa cccacaccaa cgagttcatc
     4621 acctggagtc agaatggaca aagttttctg gtcttggatg agcaaagatt tgcaaaggaa
     4681 attcttccta agtacttcaa acacaataac atggcgagct ttgtgagaca actaaatatg
     4741 tatggcttcc gaaaagtagt gcatatcgaa tctggaatta tcaaacagga aagagatggc
     4801 cctgttgaat ttcagcatcc ttatttcaag caaggccagg atgacctgtt ggagaacatt
     4861 aaaaggaagg tttcatcttc aaaaccagag gaaaataaaa ttcgtcagga agatttaaca
     4921 aaaattatta gtagtgctca gaaggttcaa ataaaacaag aaactattga gtccaggctt
     4981 tcagaattaa aaagtgagaa tgaatccctt tggaaggagg tgtcagaact aagagcaaag
     5041 catgcccagc agcaacaagt tattcggaag attgtccagt ttattgttac attggttcag
     5101 aataatcaac ttgtgagttt aaaacgtaaa aggcctctac ttctaaacac aaatggagcc
     5161 ccaaagaaga atctatatca gcacatagtc aaagaaccaa ctgataatca ccatcataaa
     5221 gttccacaca gcaggactga aggtttaaag tcaagagaac ggatttcaga tgacataatt
     5281 atttatgatg ttactgacga taatgtggat gaagaaaata ttccagttat tccagaaaca
     5341 aatgaggatg ttgtagtgga ttcctccaac cagtatcctg acattgtcat tgttgaagat
     5401 gacaacgagg atgagtatgc tcctgtcatt cagagtggag agcagagtga accagccaga
     5461 gaacccttac gtgtggggag tgctggcagc agcagccctc tcatgtctag tgctgtccag
     5521 ctaaacggct cctccagtct gacctcagaa gaccctgtga ccatgatgga ctccattctg
     5581 aatgacaaca ttaacctgtt aggaaaggtt gagctgttgg attaccttga cagtattgat
     5641 tgcagtttag aggacttcca agctatgctc tcaggaagac agtttagcat agacccagat
     5701 cttctggttg attctgagaa taagggacta gaagctacca agagcagtgt tgttcaacat
     5761 gtgtcagaag agggaagaaa atctaaatcc aagccagaca aacaacttat ccagtatact
     5821 gcctttccac ttcttgcatt cctggatggg aactctgcat ctgctattga acaggggagt
     5881 acaactgcat cgtcagaagt tgtgccttct gtagataaac ccatagaagt cgatgagctc
     5941 ctggatagca gcctggatcc agaaccgacc cagagtaagc ttgtccgcct ggaaccattg
     6001 actgaagcgg aagctagtga agccacactc ttctatttat gtgaacttgc tcctgcacct
     6061 ctggatagtg atatgccgct tttagatagt taagcggccg cgcccctctc cctccccccc
     6121 ccntaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     6181 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     6241 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     6301 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     6361 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     6421 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     6481 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     6541 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     6601 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     6661 aaaaacacga taatacctcc ggaatgattg aacaagatgg attgcacgca ggttctccgg
     6721 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     6781 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     6841 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccgcga
     6901 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     6961 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     7021 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     7081 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     7141 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     7201 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     7261 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     7321 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     7381 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     7441 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagt cgacaatcaa cctctggatt
     7501 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     7561 gatacgctgc tttaatgcct ttgtatcatg cgttaactaa acttgtttat tgcagcttat
     7621 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7681 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gaattgactc
     7741 aaatgatgtc aattagtcta tcagaagctc atctggtctc ccttccgggg gacaagacat
     7801 ccctgtttaa tatttaaaca gcagtgttcc caaactgggt tcttatatcc cttgctctgg
     7861 tcaaccaggt tgcagggttt cctgtcccca caggaacgaa gtccctaaag aaacagtggc
     7921 agccaggttt agccccggaa ttgactggat tcctttttta gggcccattg gtatggcttt
     7981 ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa
     8041 agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggcacgggga
     8101 tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgccgc cccctagcgg
     8161 gggagggacg taattacatc cctgggggct ttgggggggg ggctgtcccc gatatctata
     8221 acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata acaatataat
     8281 tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca
     8341 cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg
     8401 agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa
     8461 agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt
     8521 taatctagct gcatcaggat catatcgtcg ggtctttttt ccggctcagt catcgcccaa
     8581 gctggcgcta tctgggcatc ggggaggaag aagcccgtgc cttttcccgc gaggttgaag
     8641 cggcatggaa agagtttgcc gaggatgact gctgctgcat tgacgttgag cgaaaacgca
     8701 cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt gaactgttcg
     8761 ttcaggccac ctgggatacc agttcgtcgc ggcttttccg gacacagttc cggatggtca
     8821 gcccgaagcg catcagcaac ccgaacaata ccggcgacag ccggaactgc cgtgccggtg
     8881 tgctgattaa tgacagcggt gcggcgctgg gatattacgt cagcgaggac gggtatcctg
     8941 gctggatgcc gcagaaatgg acatggttac cccgtgagtt acccggcggg cgcgcttggc
     9001 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     9061 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     9121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     9181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     9241 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     9301 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     9361 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9421 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9481 gacaggac
//
