LOCUS       sequence-392979-        9437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of full length HSF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Price RM, Budzynski MA, Shen J, Mitchell JE, Kwan JZJ, Teves SS
  TITLE     Heat shock transcription factors demonstrate a distinct mode of 
            interaction with mitotic chromosomes.
  JOURNAL   Nucleic Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304.
  PUBMED    37114996
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304."
COMMENT     Sequence Label: HA-Halo-HSF2
FEATURES             Location/Qualifiers
     source          1..9437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     481..500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(719..823)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(849..1304)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(986..1007)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1198..1217
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1431..1453
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1445..1462
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1446..1462
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1592..1654
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2107..2318
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2331..2599
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2625..2651
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2655..2720
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2730..3620
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             3633..3653
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     misc_feature    5229..5801
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5396..5413)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5623..5642
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5810..6604
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5864..5883)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6474..6493
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6664..6684)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6726..6847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6763..6782)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6817..6836
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7609..7643
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8134..8150)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8134..8150)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8147..8169)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8158..8174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8182..8188,8189..8206,8207..8212))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8227..8248
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8365..8382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8536..9124)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8616..8635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9295..9437,1..649,650..718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHSMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEVDKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
       61 gcagaagtgg tcctgcaact ttatccacct ccatccagtc tattaattgt tgccgggaag
      121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
      181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
      241 ggcgagttac atgatccccc atgctgtgca aaaaagcggt tagctccttc ggtcctccga
      301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
      361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
      421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
      481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
      541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
      601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
      661 ggaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
      721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
      781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
      841 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaagt
      901 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      961 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     1021 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     1081 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     1141 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     1201 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     1261 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     1321 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     1381 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     1441 acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt cacgtttttg aacccgtgga
     1501 ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg atggagcaga tgaagatgct
     1561 cgacacgctg cagaacacgc agctagatta accctagaaa gataatcata ttgtgacgta
     1621 cgttaaagat aatcatgtgt aaaattgacg catgtgtttt atcggtctgt atatcgaggt
     1681 ttacttatta atttgaatag atattaagtt ttattatatt tacacttaca tactaataat
     1741 aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaacaa aaactcaaaa
     1801 tttcttctat aaagtaacaa aacttttatg agggacagcc cccccccaaa gcccccaggg
     1861 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     1921 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     1981 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     2041 cctgggggga tacggggaaa aggcctccac ggccaaggat ctgcgatcgc tccggtgccc
     2101 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggccggca
     2161 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     2221 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     2281 acgttccttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
     2341 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
     2401 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
     2461 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
     2521 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
     2581 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac tatgtaccca tacgatgttc
     2641 cagattacgc tatggactac aaagaccatg acggtgatta taaagatcat gacatcgatt
     2701 acaaggatga cgatgacaag cttggctcca tggcagaaat cggtactggc tttccattcg
     2761 acccccatta tgtggaagtc ctgggcgagc gcatgcacta cgtcgatgtt ggtccgcgcg
     2821 atggcacccc tgtgctgttc ctgcacggta acccgacctc ctcctacgtg tggcgcaaca
     2881 tcatcccgca tgttgcaccg acccatcgct gcattgctcc agacctgatc ggtatgggca
     2941 aatccgacaa accagacctg ggttatttct tcgacgacca cgtccgcttc atggatgcct
     3001 tcatcgaagc cctgggtctg gaagaggtcg tcctggtcat tcacgactgg ggctccgctc
     3061 tgggtttcca ctgggccaag cgcaatccag agcgcgtcaa aggtattgca tttatggagt
     3121 tcatccgccc tatcccgacc tgggacgaat ggccagaatt tgcccgcgag accttccagg
     3181 ccttccgcac caccgacgtc ggccgcaagc tgatcatcga tcagaacgtt tttatcgagg
     3241 gtacgctgcc gatgggtgtc gtccgcccgc tgactgaagt cgagatggac cattaccgcg
     3301 agccgttcct gaatcctgtt gaccgcgagc cactgtggcg cttcccaaac gagctgccaa
     3361 tcgccggtga gccagcgaac atcgtcgcgc tggtcgaaga atacatggac tggctgcacc
     3421 agtcccctgt cccgaagctg ctgttctggg gcaccccagg cgttctgatc ccaccggccg
     3481 aagccgctcg cctggccaaa agcctgccta actgcaaggc tgtggacatc ggcccgggtc
     3541 tgaatctgct gcaagaagac aacccggacc tgatcggcag cgagatcgcg cgctggctgt
     3601 cgacgctcga gatttccggc gagccaacca ctgaggatct gtactttcag agcgataact
     3661 ctagaatgaa gcagagttcc aacgtgccgg ctttcctcag caagctgtgg acgcttgtgg
     3721 aggaaaccca caccaacgag ttcatcacct ggagtcagaa tggacaaagt tttctggtct
     3781 tggatgagca aagatttgca aaggaaattc ttcctaagta cttcaaacac aataacatgg
     3841 cgagctttgt gagacaacta aatatgtatg gcttccgaaa agtagtgcat atcgaatctg
     3901 gaattatcaa acaggaaaga gatggccctg ttgaatttca gcatccttat ttcaagcaag
     3961 gccaggatga cctgttggag aacattaaaa ggaaggtttc atcttcaaaa ccagaggaaa
     4021 ataaaattcg tcaggaagat ttaacaaaaa ttattagtag tgctcagaag gttcaaataa
     4081 aacaagaaac tattgagtcc aggctttcag aattaaaaag tgagaatgaa tccctttgga
     4141 aggaggtgtc agaactaaga gcaaagcatg cccagcagca acaagttatt cggaagattg
     4201 tccagtttat tgttacattg gttcagaata atcaacttgt gagtttaaaa cgtaaaaggc
     4261 ctctacttct aaacacaaat ggagccccaa agaagaatct atatcagcac atagtcaaag
     4321 aaccaactga taatcaccat cataaagttc cacacagcag gactgaaggt ttaaagtcaa
     4381 gagaacggat ttcagatgac ataattattt atgatgttac tgacgataat gtggatgaag
     4441 aaaatattcc agttattcca gaaacaaatg aggatgttgt agtggattcc tccaaccagt
     4501 atcctgacat tgtcattgtt gaagatgaca acgaggatga gtatgctcct gtcattcaga
     4561 gtggagagca gagtgaacca gccagagaac ccttacgtgt ggggagtgct ggcagcagca
     4621 gccctctcat gtctagtgct gtccagctaa acggctcctc cagtctgacc tcagaagacc
     4681 ctgtgaccat gatggactcc attctgaatg acaacattaa cctgttagga aaggttgagc
     4741 tgttggatta ccttgacagt attgattgca gtttagagga cttccaagct atgctctcag
     4801 gaagacagtt tagcatagac ccagatcttc tggttgattc tgagaataag ggactagaag
     4861 ctaccaagag cagtgttgtt caacatgtgt cagaagaggg aagaaaatct aaatccaagc
     4921 cagacaaaca acttatccag tatactgcct ttccacttct tgcattcctg gatgggaact
     4981 ctgcatctgc tattgaacag gggagtacaa ctgcatcgtc agaagttgtg ccttctgtag
     5041 ataaacccat agaagtcgat gagctcctgg atagcagcct ggatccagaa ccgacccaga
     5101 gtaagcttgt ccgcctggaa ccattgactg aagcggaagc tagtgaagcc acactcttct
     5161 atttatgtga acttgctcct gcacctctgg atagtgatat gccgctttta gatagttaag
     5221 cggccgcgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     5281 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     5341 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     5401 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     5461 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     5521 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     5581 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     5641 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     5701 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     5761 aaccacgggg acgtggtttt cctttgaaaa acacgataat acctccggaa tgattgaaca
     5821 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     5881 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     5941 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     6001 agcgcggcta tcgtggctgg ccgcgacggg cgttccttgc gcagctgtgc tcgacgttgt
     6061 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     6121 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     6181 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     6241 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     6301 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     6361 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     6421 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     6481 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     6541 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     6601 ctgagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6661 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgcgtt
     6721 aactaaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6781 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6841 tatcttatca tgtctggaat tgactcaaat gatgtcaatt agtctatcag aagctcatct
     6901 ggtctccctt ccgggggaca agacatccct gtttaatatt taaacagcag tgttcccaaa
     6961 ctgggttctt atatcccttg ctctggtcaa ccaggttgca gggtttcctg tcctcacagg
     7021 aacgaagtcc ctaaagaaac agtggcagcc aggtttagcc ccggaattga ctggattcct
     7081 tttttagggc ccattggtat ggctttttcc ccgtatcccc ccaggtgtct gcaggctcaa
     7141 agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct
     7201 gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg
     7261 ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg
     7321 ggggggctgt ccctgatatc tataacaaga aaatatatat ataataagtt atcacgtaag
     7381 tagaacatga aataacaata taattatcgt atgagttaaa tcataaaagt cacgtaaaag
     7441 ataaccatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc
     7501 gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca
     7561 gcgacggatt cgcgctattt agaaagagag aacaatattt caagaatgca tgcgtcaatt
     7621 ttacgcagac tatctttcta gggttaatct agctgcatca ggatcatatc gtcgggtctt
     7681 ttttccggct cagtcatcgc ccaagctggc gctatctggg catcggggag gaagaagccc
     7741 gtgcctttac ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat gactgctgct
     7801 gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt gtggccatgc
     7861 acgcctttaa cggtgaactg ttcgttcagg ccacctggga taccagttcg tcgcggcttt
     7921 tccggacaca gttccggatg gtcagcccga agcgcatcag caacccgaac aataccggcg
     7981 acagccggaa ctgccgtgcc ggtgtgcaga ttaatggcag cggtgcggcg ctgggatatt
     8041 acgtcagcga ggacgggtat cctggctgga tgccgcagaa atggacatgg ataccccgtg
     8101 agttacccgg cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8161 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8221 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8281 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8341 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8401 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8461 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8521 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg
     8581 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8701 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8881 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8941 cttgaagtgg tggcctaact gcggctacac tagaagaaca gtatttggta tctgcgctct
     9001 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9061 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9121 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9181 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9241 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9301 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9361 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9421 tgcaatgata ccgcgag
//
