LOCUS       sequence-392987-        9236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpress METTL16.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9236)
  TITLE     Chen lab plasmids Dec 2022
REFERENCE   2  (bases 1 to 9236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pmiRNA1-OE-METTL16 F187G-sgMETTL16-MUT1
FEATURES             Location/Qualifiers
     source          1..9236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1918
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2008..2211
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2162..2182
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2208..2232
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2282..2291
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        4018..4229
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4242..4510
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      4539..4548
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4569..5231
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     misc_feature    5310..5898
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5363..5383)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5781..5792)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5972..6205
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6277..6398
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6314..6333)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6368..6387
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6438..6573
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6606..6622)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6606..6622)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6619..6641)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6630..6646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6654..6660,6661..6678,6679..6684))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6699..6720
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6837..6854)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7008..7596)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7767..8558,8559..8627))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNNLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8390..8409
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8628..8732)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8800..8818
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8856..8878)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8978..8997
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9191..9213
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9205..9222
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9206..9222
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccactgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgatactagt attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1981 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2041 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2101 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2161 acgcaaatgg gcggtaggcg tgtacggtgg gaggtttata taagcagagc tcgtttagtg
     2221 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagattctag
     2281 agccaccatg gctctgagta aatcaatgca tgcaagaaat agatacaagg acaaacctcc
     2341 tgactttgca tatctggcat ccaaatatcc agattttaag cagcatgttc agataaatct
     2401 gaatggaaga gtgagcctta attttaaaga ccccgaagca gtcagagctc tgacgtgtac
     2461 tctcctaagg gaagattttg gactttctat tgatattcca ttggagagac taattcccac
     2521 agttcccttg cgcttgaatt acatccactg ggtagaagat ctgatcggtc accaggattc
     2581 tgacaaaagt actctccgaa gaggaattga cataggcacg ggggcatctt gcatctaccc
     2641 cttacttgga gcaaccttga atggctggta ctttttggct accgaagtgg atgatatgtg
     2701 tttcaactat gcaaagaaaa atgtggaaca gaataactta tctgatctca taaaagtggt
     2761 gaaagtgcca cagaagacac tcctgatgga tgctcttaaa gaagaatctg agataatcta
     2821 tgacttttgc atgtgcaacc ctcccggttt tgccaatcaa ttggaagcca agggagtaaa
     2881 ctcacgaaat cctcgaagac ctccgcctag ttctgttaat acaggaggca tcacagagat
     2941 catggcagaa ggaggtgaat tagagtttgt taaaaggatc atccatgaca gtctacaact
     3001 taaaaaaaga ttaagatggt atagctgcat gctgggaaag aaatgcagcc tggcgcctct
     3061 gaaggaggag cttcgcatac aaggggttcc caaagtaacg tacactgaat tctgtcaagg
     3121 tcggacaatg agatgggcct tagcttggag tttttatgat gatgtcacag taccatcacc
     3181 accaagtaag cgaagaaaat tagagaaacc gagaaaaccc ataacattcg tggtgctggc
     3241 gtccgtgatg aaggaattat ccctcaaagc atcacctctg cgctcggaga cggcggaagg
     3301 catagtcgtt gtcacgacat ggattgaaaa aattctcact gatttgaagg tccagcataa
     3361 acgagttccc tgtggaaaag aggaagtcag ccttttccta acggccatag aaaactcctg
     3421 gattcattta aggagaaaga aaagagagcg tgtgagacag ctgagagaag ttccccgagc
     3481 tcctgaggac gtcattcagg ccttggaaga gaaaaagccc acccccaaag agtctggcaa
     3541 tagccaagaa cttgccaggg gcccccagga gaggaccccc tgtgggcctg ctctgcggga
     3601 aggcgaggct gccgctgtgg agggcccgtg cccgagccag gagtccctgt cccaggagga
     3661 aaacccggaa cccacggagg atgaaaggag tgaggaaaag ggaggggtgg aggttttgga
     3721 aagttgtcaa ggctctagca acggagccca ggaccaagag gcttctgagc agttcggcag
     3781 cccagtggct gaaaggggga aacgtctccc aggagtggcc ggacagtacc tgtttaagtg
     3841 tttgataaac gttaagaagg aggtggacga tgccttagtg gagatgcact gggttgaggg
     3901 ccagaacagg gatctgatga accagctttg cacctacata cgtaaccaaa ttttcaggct
     3961 tgttgcagtt aactaggcgg ccgcgaagga tctgcgatcg ctccggtgcc cgtcagtggg
     4021 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaacgg
     4081 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     4141 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     4201 ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc atctctcctt
     4261 cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc
     4321 ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt
     4381 cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca
     4441 cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc
     4501 gttacagatc caagctgtga ccggcgccta cgctagacgc caccatggag agcgacgaga
     4561 gcggcctgcc cgccatggag atcgagtgcc gcatcaccgg caccctgaac ggcgtggagt
     4621 tcgagctggt gggcggcgga gagggcaccc ccaagcaggg ccgcatgacc aacaagatga
     4681 agagcaccaa aggcgccctg accttcagcc cctacctgct gagccacgtg atgggctacg
     4741 gcttctacca cttcggcacc taccccagcg gctacgagaa ccccttcctg cacgccatca
     4801 acaacggcgg ctacaccaac acccgcatcg agaagtacga ggacggcggc gtgctgcacg
     4861 tgagcttcag ctaccgctac gaggccggcc gcgtgatcgg cgacttcaag gtggtgggca
     4921 ccggcttccc cgaggacagc gtgatcttca ccgacaagat catccgcagc aacgccaccg
     4981 tggagcacct gcaccccatg ggcgataacg tgctggtggg cagcttcgcc cgcaccttca
     5041 gcctgcgcga cggcggctac tacagcttcg tggtggacag ccacatgcac ttcaagagcg
     5101 ccatccaccc cagcatcctg cagaacgggg gccccatgtt cgccttccgc cgcgtggagg
     5161 agctgcacag caacaccgag ctgggcatcg tggagtacca gcacgccttc aagaccccca
     5221 ttgccttcgc cagatcccgc gctcagtcgt ccaattctgc cgtggacggc accgccggac
     5281 ccggctccac cggatctcgc taagtcgaca atcaacctct ggattacaaa atttgtgaaa
     5341 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5401 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5461 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5521 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5581 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5641 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5701 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5761 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5821 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5881 tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat
     5941 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     6001 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     6061 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6121 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6181 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     6241 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     6301 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6361 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     6421 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6481 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     6541 tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac caaattcgta
     6601 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6661 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6721 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6781 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6841 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6901 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6961 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7021 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7081 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7141 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7201 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7261 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7321 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7381 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7441 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7501 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7561 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7621 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7681 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7741 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7801 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7861 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7921 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7981 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8041 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8101 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8161 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8221 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8281 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gttattctga
     8341 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8401 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8461 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8521 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8581 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8641 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8701 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8761 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     8821 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     8881 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     8941 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     9001 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     9061 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     9121 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     9181 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct gacgcg
//
