LOCUS       sequence-392988-        9166 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express the AsCas12a nuclease variant ""AsCas12a-Plus"" that 
            enhanced the editing activity and specificity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9166)
  AUTHORS   Huang H, Huang G, Tan Z, Hu Y, Shan L, Zhou J, Zhang X, Ma S, Lv W, 
            Huang T, Liu Y, Wang D, Zhao X, Lin Y, Rong Z
  TITLE     Engineered Cas12a-Plus nuclease enables gene editing with enhanced 
            activity and specificity.
  JOURNAL   BMC Biol. 2022 Apr 25;20(1):91. doi: 10.1186/s12915-022-01296-1.
  PUBMED    35468792
REFERENCE   2  (bases 1 to 9166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2022-04-25"; volume: "20(1):91. doi"; pages: " 
            10.1186/s12915-022-01296-1"
COMMENT     Sequence Label: pCAG-AsCas12a-Plus-mCherry
FEATURES             Location/Qualifiers
     source          1..9166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        11..390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        392..667
                     /label=chicken beta-actin promoter
     intron          668..1684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1608..1630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1692..1711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1739..1757
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1739..1756
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1758..1777
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1758..1776
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1778..5698
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKEKVAAAQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      5418..5427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5699..5746
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5753..5833
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5858..5914
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5921..6631
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6066..6084)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6334..6353
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6674..6795
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6711..6730)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6765..6784
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6844..6860)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6844..6860)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6857..6879)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6868..6884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6892..6898,6899..6916,6917..6922))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6937..6958
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7075..7092)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7246..7834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7326..7345)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8005..8796,8797..8865))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8628..8647
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8866..8970)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9049..9068)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aattcgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
       61 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      121 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      181 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      241 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      301 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      361 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
      421 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
      481 tgtgcagcga tgggggcggg gggggggggg gggcgcgcca ggsggggsgg ggsggggsga
      541 ggggsggggs ggggsgaggs ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
      601 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
      661 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
      721 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
      781 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
      841 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
      901 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
      961 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     1021 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     1081 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     1141 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     1201 ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg
     1261 cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag
     1321 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     1381 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     1441 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     1501 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     1561 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     1621 gcgtgtgacc ggcggctcta gcgcctctgc taaccatgtt catgccttct tctttttcct
     1681 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattcat
     1741 ttaggtgaca ctatagataa tacgactcac tatagggatg acacagttcg agggctttac
     1801 caacctgtat caggtgagca agacactgcg gtttgagctg atcccacagg gcaagaccct
     1861 gaagcacatc caggagcagg gcttcatcga ggaggacaag gcccgcaatg atcactacaa
     1921 ggagctgaag cccatcatcg atcggatcta caagacctat gccgaccagt gcctgcagct
     1981 ggtgcagctg gattgggaga acctgagcgc cgccatcgac tcctatagaa aggagaaaac
     2041 cgaggagaca aggaacgccc tgatcgagga gcaggccaca tatcgcaatg ccatccacga
     2101 ctacttcatc ggccggacag acaacctgac cgatgccatc aataagagac acgccgagat
     2161 ctacaagggc ctgttcaagg ccgagctgtt taatggcaag gtgctgaagc agctgggcac
     2221 cgtgaccaca accgagcacg agaacgccct gctgcggagc ttcgacaagt ttacaaccta
     2281 cttctccggc ttttatagaa acaggaagaa cgtgttcagc gccgaggaca ttagcacagc
     2341 catcccacac cgcatcgtgc aggacaactt ccccaagttt aaggagaatt gtcacatctt
     2401 cacacgcctg atcaccgccg tgcccagcct gcgggagcac tttgagaacg tgaagaaggc
     2461 catcggcatc ttcgtgagca cctccatcga ggaggtgttt tccttccctt tttataacca
     2521 gctgctgaca cagacccaga tcgacctgta taaccagctg ctgggaggaa tctctcggga
     2581 ggcaggcacc gagaagatca agggcctgaa cgaggtgctg aatctggcca tccagaagaa
     2641 tgatgagaca gcccacatca tcgcctccct gccacacaga ttcatccccc tgtttaagca
     2701 gatcctgtcc gataggaaca ccctgtcttt catcctggag gagtttaaga gcgacgagga
     2761 agtgatccag tccttctgca agtacaagac actgctgaga aacgagaacg tgctggagac
     2821 agccgaggcc ctgtttaacg agctgaacag catcgacctg acacacatct tcatcagcca
     2881 caagaagctg gagacaatca gcagcgccct gtgcgaccac tgggatacac tgaggaatgc
     2941 cctgtatgag cggagaatct ccgagctgac aggcaagatc accaagtctg ccaaggagaa
     3001 ggtgcagcgc agcctgaagc acgaggatat caacctgcag gagatcatct ctgccgcagg
     3061 caaggagctg agcgaggcct tcaagcagaa aaccagcgag atcctgtccc acgcacacgc
     3121 cgccctggat cagccactgc ctacaaccct gaagaagcag gaggagaagg agatcctgaa
     3181 gtctcagctg gacagcctgc tgggcctgta ccacctgctg gactggtttg ccgtggatga
     3241 gtccaacgag gtggaccccg agttctctgc ccggctgacc ggcatcaagc tggagatgga
     3301 gccttctctg agcttctaca acaaggccag aaattatgcc accaagaagc cctactccgt
     3361 ggagaagttc aagctgaact ttcagatgcc tacactggcc tctggctggg acgtgaataa
     3421 ggagaagaac aatggcgcca tcctgtttgt gaagaacggc ctgtactatc tgggcatcat
     3481 gccaaagcag aagggcaggt ataaggccct gagcttcgag cccacagaga aaaccagcga
     3541 gggctttgat aagatgtact atgactactt ccctgatgcc gccaagatga tcccaaagtg
     3601 cagcacccag ctgaaggccg tgacagccca ctttcagacc cacacaaccc ccatcctgct
     3661 gtccaacaat ttcatcgagc ctctggagat cacaaaggag atctacgacc tgaacaatcc
     3721 tgagaaggag ccaaagaagt ttcagacagc ctacgccaag aaaaccggcg accagaaggg
     3781 ctacagagag gccctgtgca agtggatcga cttcacaagg gattttctgt ccaagtatac
     3841 caagacaacc tctatcgatc tgtctagcct gcggccatcc tctcagtata aggacctggg
     3901 cgagtactat gccgagctga atcccctgct gtaccacatc agcttccaga gaatcgccga
     3961 gaaggagatc atggatgccg tggagacagg caagctgtac ctgttccaga tctataacaa
     4021 ggactttgcc aagggccacc acggcaagcc taatctgcac acactgtatt ggaccggcct
     4081 gttttctcca gagaacctgg ccaagacaag catcaagctg aatggccagg ccgagctgtt
     4141 ctaccgccct aagtccagga tgaagaggat ggcacaccgg ctgggagaga agatgctgaa
     4201 caagaagctg aaggatcaga aaaccccaat ccccgacacc ctgtaccagg agctgtacga
     4261 ctatgtgaat cacagactgt cccacgacct gtctgatgag gccagggccc tgctgcccaa
     4321 cgtgatcacc aaggaggtgt ctcacgagat catcaaggat aggcgcttta ccagcgacaa
     4381 gttctttttc cacgtgccta tcacactgaa ctatcaggcc gccaattccc catctaagtt
     4441 caaccagagg gtgaatgcct acctgaagga gcaccccgag acacctatca tcggcatcga
     4501 tcggggcgag agaaacctga tctatatcac agtgatcgac tccaccggca agatcctgga
     4561 gcagcggagc ctgaacacca tccagcagtt tgattaccag aagaagctgg acaacaggga
     4621 gaaggagaag gtggcggccg ctcaggcctg gtctgtggtg ggcacaatca aggatctgaa
     4681 gcagggctat ctgagccagg tcatccacga gatcgtggac ctgatgatcc actaccaggc
     4741 cgtggtggtg ctggagaacc tgaatttcgg ctttaagagc aagaggaccg gcatcgccga
     4801 gaaggccgtg taccagcagt tcgagaagat gctgatcgat aagctgaatt gcctggtgct
     4861 gaaggactat ccagcagaga aagtgggagg cgtgctgaac ccataccagc tgacagacca
     4921 gttcacctcc tttgccaaga tgggcaccca gtctggcttc ctgttttacg tgcctgcccc
     4981 atatacatct aagatcgatc ccctgaccgg cttcgtggac cccttcgtgt ggaaaaccat
     5041 caagaatcac gagagccgca agcacttcct ggagggcttc gactttctgc actacgacgt
     5101 gaaaaccggc gacttcatcc tgcactttaa gatgaacaga aatctgtcct tccagagggg
     5161 cctgcccggc tttatgcctg catgggatat cgtgttcgag aagaacgaga cacagtttga
     5221 cgccaagggc acccctttca tcgccggcaa gagaatcgtg ccagtgatcg agaatcacag
     5281 attcaccggc agataccggg acctgtatcc tgccaacgag ctgatcgccc tgctggagga
     5341 gaagggcatc gtgttcaggg atggctccaa catcctgcca aagctgctgg agaatgacga
     5401 ttctcacgcc atcgacacca tggtggccct gatccgcagc gtgctgcaga tgcggaactc
     5461 caatgccgcc acaggcgagg actatatcaa cagccccgtg cgcgatctga atggcgtgtg
     5521 cttcgactcc cggtttcaga acccagagtg gcccatggac gccgatgcca atggcgccta
     5581 ccacatcgcc ctgaagggcc agctgctgct gaatcacctg aaggagagca aggatctgaa
     5641 gctgcagaac ggcatctcca atcaggactg gctggcctac atccaggagc tgcgcaacaa
     5701 aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagggat cctacccata
     5761 cgatgttcca gattacgctt atccctacga cgtgcctgat tatgcatacc catacgatgt
     5821 ccccgactat gccctcgaga gcaccggtgg cagcggagct actaacttca gcctgctgaa
     5881 gcaggctgga gacgtggagg agaaccctgg acctgccggt atggtgagca agggcgagga
     5941 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     6001 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     6061 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     6121 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     6181 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     6241 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     6301 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     6361 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     6421 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     6481 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     6541 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     6601 caccggcggc atggacgagc tgtacaagta actgcagcgc ggggatctca tgctggagtt
     6661 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6721 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6781 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     6841 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6901 agccggaagc ataaagtgta aagcctaggg tgcctaatga gtgagctaac tcacattaat
     6961 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7021 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     7081 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     7141 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7201 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7261 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7321 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7381 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7441 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7501 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7561 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7621 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7681 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7741 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7801 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7861 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7921 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7981 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8041 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8101 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8161 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8221 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8281 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8341 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8401 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8461 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8521 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8581 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8641 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8701 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8761 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8821 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8881 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8941 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9001 cgacggatcg ggagatcgat ctcccgatcc cctagggtcg actctcagta caatctgctc
     9061 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     9121 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgac
//
