LOCUS       sequence-392990-        7157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of the mammalian NDP52 tagged with HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7157)
  AUTHORS   Nguyen TN, Sawa-Makarska J, Khuu G, Lam WK, Adriaenssens E, 
            Fracchiolla D, Shoebridge S, Bernklau D, Padman BS, Skulsuppaisarn 
            M, Lindblom RSJ, Martens S, Lazarou M
  TITLE     Unconventional initiation of PINK1/Parkin mitophagy by Optineurin.
  JOURNAL   Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 
            10.1016/j.molcel.2023.04.021.
  PUBMED    37207627
REFERENCE   2  (bases 1 to 7157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.04"; journalName: "Mol Cell"; date: 
            "2023-05-18- 18"; volume: "83"; issue: "10"; pages: "1693-1709.e9"
COMMENT     Sequence Label: pBMN HA-NDP52
FEATURES             Location/Qualifiers
     source          1..7157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             189..658
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(788..805)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(959..1547)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1039..1058)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1711..2502,2503..2571))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2334..2353
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2572..2676)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  2821..3588
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     LTR             3990..4459
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4524..4881
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             4946..5362
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5292..5314
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5330..5346
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    5372..5746
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     5684..5700
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             5758..5784
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 caccggatct agataactga tcacgataaa ataaaagatt ttatttagtc tccagaaaaa
       61 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
      121 caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga
      181 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
      241 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      301 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
      361 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
      421 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
      481 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
      541 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
      601 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcaca
      661 tgcagcatgt atcaaaatta atttggtttt ttttcttaag tatttacatt aaatggccat
      721 agttgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      781 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      841 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      901 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      961 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1021 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1081 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1141 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1201 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1261 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1321 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1381 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1441 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1501 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1561 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1621 tgagattatc aaaaaggatc ttcacctaga tccttttgcg gccggccgca aatcaatcta
     1681 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     1741 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     1801 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     1861 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     1921 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     1981 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     2041 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     2101 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     2161 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     2221 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     2281 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     2341 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     2401 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     2461 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     2521 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     2581 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     2641 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     2701 agctttgctc ttaggagttt cctaatacat cccaaactca aatatataaa gcatttgact
     2761 tgttctatgc cctagggggc ggggggaagc taagccagct ttttttaaca tttaaaatgt
     2821 ggtaatgact ccaacttatt gatagtgttt tatgttcaga taatgcccga tgactttgtc
     2881 atgcagctcc accgattttg agaacgacag cgacttccgt cccagccgtg ccaggtgctg
     2941 cctcagattc aggttatgcc gctcaattcg ctgcgtatat cgcttgctga ttacgtgcag
     3001 ctttcccttc aggcgggatt catacagcgg ccagccatcc gtcatccata tcaccacgtc
     3061 aaagggtgac agcaggctca taagacgccc cagcgtcgcc atagtgcgtt caccgaatac
     3121 gtgcgcaaca accgtcttcc ggagactgtc atacgcgtaa aacagccagc gctggcgcga
     3181 tttagccccg acatagcccc actgttcgtc catttccgcg cagacgatga cgtcactgcc
     3241 cggctgtatg cgcgaggtta ccgactgcgg cctgagtttt ttaagtgacg taaaatcgtg
     3301 ttgaggccaa cgcccataat gcgggctgtt gcccggcatc caacgccatt catggccata
     3361 tcaatgattt tctggtgcgt accgggttga gaagcggtgt aagtgaactg cagttgccat
     3421 gttttacggc agtgagagca gagatagcgc tgatgtccgg cggtgctttt gccgttacgc
     3481 accaccccgt cagtagctga acaggaggga cagctgatag aaacagaagc cactggagca
     3541 cctcaaaaac accatcatac actaaatcag taagttggca gcatcacctt aaaatgttaa
     3601 ttccatttta aatgcacaga tgtttttatt tcataagggt ttcaatgtgc atgaatgctg
     3661 caatattcct gttaccaaag ctagtataaa taaaaataga taaacgtgga aattacttag
     3721 agtttctgtc attaacgttt ccttcctcag ttgacaacat aaatgcgctg ctgagaagcc
     3781 agtttgcatc tgtcaggatc aatttcccat tatgccagtc atattaatta ctagtcaatt
     3841 agttgatttt tatttttgac atatacatgt gaaagacccc acctgtaggt ttggcaagct
     3901 agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa tagaaaagtt
     3961 cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     4021 aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa
     4081 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
     4141 gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
     4201 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
     4261 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggcgcgc
     4321 cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt
     4381 tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
     4441 cgtcagcggg ggtctttcat ttgggggctc gtccgggatc gggagacccc tgcccaggga
     4501 ccaccgaccc accaccggga ggtaagctgg ccagcaactt atctgtgtct gtccgattgt
     4561 ctagtgtcta tgactgattt tatgcgcctg cgtcggtact agttagctaa ctagctctgt
     4621 atctggcgga cccgtggtgg aactgacgag ttcggaacac ccggccgcaa ccctgggaga
     4681 cgtcccaggg acttcggggg ccgtttttgt ggcccgacct gagtccaaaa atcccgatcg
     4741 ttttggactc tttggtgcac cccccttaga ggagggatat gtggttctgg taggagacga
     4801 gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttggg accgaagccg
     4861 cgccgcgcgt cttgtctgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct
     4921 gtatttgtct gaaaatatgg gcccgggcca gactgttacc actcccttaa gtttgacctt
     4981 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
     5041 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
     5101 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
     5161 gcatggacac ccagactagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
     5221 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
     5281 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
     5341 cctcactcct tctctaggcg cccccatatg gccatatgag atcttatatg gggcaccccc
     5401 gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc cctctctcca
     5461 agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc tggcggcagc
     5521 ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg gcgacacagt
     5581 gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac cttacacagt
     5641 cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga tacacgccgc
     5701 ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgccgc caccatgtac
     5761 ccatacgatg ttccagatta cgcttccgga ctcagatctc gagctcaagc ttgggaggag
     5821 accatcaaag atccccccac atcagctgtc ttgctggatc actgtcattt ctctcaggtc
     5881 atctttaaca gtgtggagaa gttctacatc cctggagggg acgtcacatg tcattatacc
     5941 ttcacccagc atttcatccc tcgtcgaaag gattggattg gcatctttag agtggggtgg
     6001 aagacaaccc gtgagtatta caccttcatg tgggttactt tgcccattga cctaaacaac
     6061 aaatcagcta aacagcagga agtccaattc aaagcttact acctgcccaa ggatgatgag
     6121 tattaccagt tctgctatgt ggatgaggat ggtgtggtcc ggggagcaag tattcctttc
     6181 caattccgtc cagaaaatga ggaagacatc ctggttgtta ccactcaggg agaggtggaa
     6241 gagattgagc agcacaacaa ggagctttgc aaagaaaacc aggagctgaa ggacagctgt
     6301 atcagcctcc agaagcagaa ctcagacatg caggctgagc tccaaaagaa gcaggaggag
     6361 ctagaaaccc tacagagcat caataagaag ttggaactga aagtgaaaga acagaaggac
     6421 tattgggaga cagagctgct tcaactgaaa gaacaaaacc agaagatgtc ctcagaaaat
     6481 gagaagatgg gaatcagagt ggatcagctt caggcccagc tgtcaactca agagaaagaa
     6541 atggagaagc ttgttcaggg agatcaagat aagacagagc agttagagca gctgaaaaag
     6601 gaaaatgacc acctctttct cagtttagct gaacagagga aggaccagaa gaagctcgag
     6661 cagacagtgg agcaaatgaa gcagaatgaa actactgcaa tgaagaaaca acaggaatta
     6721 atggatgaaa actttgacct gtcaaaaaga ctgagtgaga acgaaattat atgtaatgct
     6781 ctgcagagac agaaagagag attggaagga gaaaatgatc ttttgaagag ggagaacagc
     6841 agattgctca gttacatggg tctggatttt aattctttgc cgtatcaagt acctacttca
     6901 gatgaaggag gcgcaagaca aaatccagga cttgcctatg gaaacccata ttctggtatc
     6961 caagaaagtt cttcccccag cccgctctcc atcaagaaat gccctatctg caaagcagat
     7021 gatatttgtg atcacacctt ggagcaacag cagatgcagc ccctttgttt caattgtcca
     7081 atttgtgaca agatcttccc agctacagag aagcagatct ttgaagacca cgtgttctgc
     7141 cactctctct gaggatc
//
