LOCUS       sequence-393000-        9704 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible Piggybac vector expressing human TYR CDS with hygromycin 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9704)
  AUTHORS   Bajpai VK, Swigut T, Mohammed J, Naqvi S, Arreola M, Tycko J, Kim 
            TC, Pritchard JK, Bassik MC, Wysocka J
  TITLE     A genome-wide genetic screen uncovers determinants of human 
            pigmentation.
  JOURNAL   Science. 2023 Aug 11;381(6658):eade6289. doi: 
            10.1126/science.ade6289. Epub 2023 Aug 11.
  PUBMED    37561850
REFERENCE   2  (bases 1 to 9704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-08-11"; volume: "381(6658):eade6289. doi"; pages: " 
            10.1126/science.ade6289. Epub 2023 Aug 11"
COMMENT     Sequence Label: Pb-TRE3G-TYR-HygR
FEATURES             Location/Qualifiers
     source          1..9704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   1482..1516
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    2279..2400
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2290..2309)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2344..2363
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2434..2454
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2511..3530)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             complement(2511..3530)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             complement(3552..3605)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3606..4349)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     LTR             4383..4651
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(4664..4875)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     polyA_signal    5106..5154
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5179..5198
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(6911..7289)
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     primer_bind     complement(6978..7002)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7047..7065
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7083..7101
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7119..7137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7155..7173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7191..7209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7227..7245
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7263..7281
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     repeat_region   7738..7800
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7930..7947)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7930..7946)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7939..7961)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8088..8543
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8175..8194)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8385..8406
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8569..8673
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8674..8742,8743..9534)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8892..8911)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg cccgccgggt
     1021 aactcacggg gtatccatgt ccatttctgc ggcatccagc caggataccc gtcctcgctg
     1081 acgtaatatc ccagcgccgc accgctgtca ttaatctgca caccggcacg gcagttccgg
     1141 ctgtcgccgg tattgttcgg gttgctgatg cgcttcgggc tgaccatccg gaactgtgtc
     1201 cggaaaagcc gcgacgaact ggtatcccag gtggcctgaa cgaacagttc accgttaaag
     1261 gcgtgcatgg ccacaccttc ccgaatcatc atggtaaacg tgcgttttcg ctcaacgtca
     1321 atgcagcagc agtcatcctc ggcaaactct ttccatgccg cttcaacctc gcgggaaaag
     1381 gcacgggctt cttcctcccc gatgcccaga tagcgccagc ttgggcgatg actgagccgg
     1441 aaaaaagacc cgacgatatg atcctgatgc agctagatta accctagaaa gatagtctgc
     1501 gtaaaattga cgcatgcatt cttgaaatat tgctctctct ttctaaatag cgcgaatccg
     1561 tcgctgtgca tttaggacat ctcagtcgcc gcttggagct cccgtgaggc gtgcttgtca
     1621 atgcggtaag tgtcactgat tttgaactat aacgaccgcg tgagtcaaaa tgacgcatga
     1681 ttatctttta cgtgactttt aagatttaac tcatacgata attatattgt tatttcatgt
     1741 tctacttacg tgataactta ttatatatat attttcttgt tatagatatc agggacagcc
     1801 cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag
     1861 ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg
     1921 gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct
     1981 gctctttgag cctgcagaca cctgggggga tacggggaaa aagccatacc aatgggccct
     2041 aaaaaaggaa tccagtcaat tccggggcta aacctggctg ccactgtttc tttagggact
     2101 tcgttcctgt gaggacagga aaccctgcaa cctggttgac cagagcaagg gatataagaa
     2161 cccagtttgg gaacactgct gtttaaatat taaacaggga tgtcttgtcc cccggaaggg
     2221 agaccagatg agcttctgat agactaattg acatcatttg agtcaattcc agacatgata
     2281 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2341 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2401 aactgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta
     2461 agaataccag tcaatctttc acaaattttg taatccagag gttccttggc ttattccttt
     2521 gccctcggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca
     2581 tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg
     2641 gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca
     2701 accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc
     2761 gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc
     2821 aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc
     2881 tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg
     2941 aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca
     3001 tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac
     3061 acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct
     3121 tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc
     3181 atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac
     3241 gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg
     3301 tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct
     3361 ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg
     3421 aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg
     3481 aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tccggatcta
     3541 tccatgaatt cagggccggg attctcctcc acgtcaccgc atgttagaag acttcctctg
     3601 ccctccccgg ggagcatgtc aaggtcaaaa tcgtcaagag cgtcagcagg cagcatatca
     3661 aggtcaaagt cgtcaagggc atcggctggg agcatgtcta agtcaaaatc gtcaagggcg
     3721 tcggtcggcc cgccgctttc gcactttagc tgtttctcca ggccacatat gattagttcc
     3781 aggccgaaaa ggaaggcagg ttcggctccc tgccggtcga acagctcaat tgcttgtttc
     3841 agaagtgggg gcatagaatc ggtggtaggt gtctctcttt cctcttttgc tacttgatgc
     3901 tcctgttcct ccaatacgca gcccagtgta aagtggccca cggcggacag agcgtacagt
     3961 gcgttctcca gggagaagcc ttgctgacac aggaacgcga gctgattttc cagggtttcg
     4021 tactgtttct ctgttgggcg ggtgccgaga tgcactttag ccccgtcgcg atgtgagagg
     4081 agagcacagc ggtatgactt ggcgttgttc cgcagaaagt cttgccatga ctcgccttcc
     4141 agggggcagg agtgggtatg atgcctgtcc agcatctcga ttggcagggc atcgagcagg
     4201 gcccgcttgt tcttcacgtg ccagtacagg gtaggctgct caactcccag cttttgagcg
     4261 agtttccttg tcgtcaggcc ttcgataccg actccattga gtaattccag agcagagttt
     4321 atgactttgc tcttgtccag tctagacatg gcggctctag agtaggcgcc ggtcacagct
     4381 tagatctgta acggcgcaga acagaaaacg aaacaaagac gtagagttga gcaagcaggg
     4441 tcaggcaaag cgtggagagc cggctgagtc taggtaggct ccaagggagc gccggacaaa
     4501 ggcccggtct cgacctgagc tttaaactta cctagacggc ggacgcagtt caggaggcac
     4561 cacaggcggg aggcggcaga acgcgactca accggcgtgg atggcggcct caggtagggc
     4621 ggcgggcgcg tgaaggagag atgcgagccc ctcgaagctt cagctgtgtt ctggcggcaa
     4681 acccgttgcg aaaaagaacg ttcacggcga ctactgcact tatatacggt tctcccccac
     4741 cctcgggaaa aaggcggagc cagtacacga catcactttc ccagtttacc ccgcgccacc
     4801 ttctctaggc acccgttcaa ttgccgaccc ctccccccaa cttctcgggg actgtgggcg
     4861 atgtgcgctc tgcccactga cgggcaccgg agcgatcgca gatccactta gatttagctg
     4921 gatccgattt cgcagatctg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc
     4981 tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg
     5041 gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg
     5101 tttatgaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt gccgtcatag
     5161 cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc cgtttaaact
     5221 cattactaac cggtggatcc cgggcccgcg gtaccgtcga ctgcagatgg ctctgataca
     5281 agctgtggta atcctctttc tccatgagga gtggctgctt ttcttcagga agctgctttc
     5341 tcttgtgacg acacagcaag ctcacaagcc ctgccagcag ggcagtgagg acggccccta
     5401 ccatcgccgc cccaaggagc catgaccaga tccgactcgc ttgttccaaa taggacttaa
     5461 tgtagtcttg aaaagagtct gggtctgaat cttgtagata gctatagtca tagcccagat
     5521 ctttggatga aataaagaaa tcaccatttc tgtacagtgg tataaaagga accatgtagg
     5581 attcccggtt atgtccaatg ggtgcattgg cttctggata aacttcttga agaggacggt
     5641 gccttcggag ccactgctca aaaatactgt caacaaatgc atggtgaaga aggaagatag
     5701 gatcgttggc agatccctgt acctgggaca ttgttccatt catatagatg tgcaaggcat
     5761 tgtgcatgct gctttgagag gcatccgcta tcccagtaag tggactagca aatccttcca
     5821 gtgtatttct aaagctgaaa ttggcagctt tatccatgga accagattca tattgggtca
     5881 aactcaggca aaattctaca tcagctgaag aggggagcct tggggttctg gatttgtcat
     5941 ggtttccagg attacgccgt aaaggtccct cgggcgttcc attgcataaa gactgatggc
     6001 tgttgtactc ctccaatcgg ctacagacaa tctgccaaga ggagaagaat gatgctgggc
     6061 tgagtaagtt aggatttgtg gggtgctgac ctcccatgta ctcatctgtg caaatgtcac
     6121 acttttctgc atcccgccag tcccaatatg gaatagtgaa gttttcatct cctgtcagct
     6181 tctggatttc ttgttcccac cgcaacaaga agagtctatg ccaaggcaga aaagctggtg
     6241 cttcatgggc aaaatcaatg tctctccaga tttcatatcc cccaagcagt gcatccattg
     6301 acacataata atgcatccag acaaagaggt cataaatatt gatgtcgtta aacatgggtg
     6361 ttgatccatt tttcatttgg ccataggtcc ctatggggat gacatagtct gagctgatgg
     6421 tatgctttgc taaagtgagg taggcaaaaa atttgtcctt ctctggggca ctcaaatcga
     6481 agatgtttct tctcaccaag agtcgtctct ctgtgcagtt tggtccccaa aagccaaact
     6541 tgcagtttcc acagttgaat cccatgaagt tgccagagca ctggcaggtc ctattataaa
     6601 agacggaagg ccacgactcc cggtcatcca cccctgtgaa gggaaattga ggcccaagtg
     6661 gtgcattgga cagaaggata ttctgacagg aacctctgcc tgaaagctgg ccacagggac
     6721 tcctgtcccc gctccacggt ggacagcatt ccttctccat caggttctta gaggagacac
     6781 aggctctagg gaaatggcca gcggaggtct ggaaactcca cagcaggcag tacaaaacag
     6841 ccaggagcat tcttcccgga attcgaagct tgagctcgag atctgagtcc ggtagcgcta
     6901 gctctagacc tttacgaggg taggaagtgg tacggaaagt tggtataaga caaaagtgtt
     6961 gtggaattgc tccaggcgat ctgacggttc actaaacgag ctctgctttt ataggcgccc
     7021 accgtacacg cctaaagctt atacgttctc tatcactgat agggagtaaa ctggatatac
     7081 gttctctatc actgataggg agtaaactgt agatacgttc tctatcactg atagggagta
     7141 aactggtcat acgttctcta tcactgatag ggagtaaact ccttatacgt tctctatcac
     7201 tgatagggag taaagtctgc atacgttctc tatcactgat agggagtaaa ctcttcatac
     7261 gttctctatc actgataggg agtaaactcg aggaattctt gaagacgaaa gactagtggc
     7321 cgtggaggcc ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag
     7381 cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg
     7441 ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct
     7501 gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc
     7561 tcataaaagt tttgttactt tatagaagaa attttgagtt tttgtttttt ttaataaata
     7621 aataaacata aataaattgt ttgttgaatt tattattagt atgtaagtgt aaatataata
     7681 aaacttaata tctattcaaa ttaataaata aacctcgata tacagaccga taaaacacat
     7741 gcgtcaattt tacacatgat tatctttaac gtacgtcaca atatgattat ctttctaggg
     7801 ttaatctagc tgcgtgttct gcagcgtgtc gagcatcttc atctgctcca tcacgctgta
     7861 aaacacattt gcaccgcgag tctgcccgtc ctccacgggt tcaaaaacgt gaatgaacga
     7921 ggcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     7981 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8041 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     8101 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8161 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8221 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8281 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8341 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8401 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     8461 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     8521 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     8581 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8641 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8701 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     8761 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     8821 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     8881 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     8941 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9001 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9061 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9121 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9181 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9241 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9301 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9361 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9421 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9481 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9541 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9601 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9661 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
