LOCUS       sequence-393004-        9015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector expressing COMMD3 CDS with hygromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Bajpai VK, Swigut T, Mohammed J, Naqvi S, Arreola M, Tycko J, Kim 
            TC, Pritchard JK, Bassik MC, Wysocka J
  TITLE     A genome-wide genetic screen uncovers determinants of human 
            pigmentation.
  JOURNAL   Science. 2023 Aug 11;381(6658):eade6289. doi: 
            10.1126/science.ade6289. Epub 2023 Aug 11.
  PUBMED    37561850
REFERENCE   2  (bases 1 to 9015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2023-08-11"; volume: "381(6658):eade6289. doi"; pages: " 
            10.1126/science.ade6289. Epub 2023 Aug 11"
COMMENT     Sequence Label: pCHA1.1-COMMD3_CDS-V5-hygro
FEATURES             Location/Qualifiers
     source          1..9015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2881..4092
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3929..3948
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4701..4742
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4787..4904
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4953..5463
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5382..5400
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5483..6520
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             6499..6507
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     LTR             6591..6824
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6896..7017
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6933..6952)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6987..7006
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7057..7192
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7212..7231)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7213..7231)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7241..7258)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7241..7257)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7250..7272)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7399..7854
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7486..7505)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7696..7717
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7880..7984
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7985..8053,8054..8845)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8203..8222)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcagcag
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg agcgggatct
     2881 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
     2941 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
     3001 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
     3061 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
     3121 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
     3181 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
     3241 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
     3301 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
     3361 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
     3421 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
     3481 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
     3541 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
     3601 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
     3661 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
     3721 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
     3781 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
     3841 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     3901 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     3961 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     4021 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     4081 tttttgttag acgaagcgct agcatggagc tctcggagtc tgtgcagaaa ggcttccaga
     4141 tgctggcgga tccccgctcc ttcgactcca acgccttcac gcttctcctc cgggcggcat
     4201 tccagagtct gctggacgcc caggcggacg aggccgtgtt agatcatcca gacttgaaac
     4261 atatcgaccc agtggtttta aaacattgtc atgcagcagc tgcaacttac atactagagg
     4321 caggaaagca ccgagctgac aagtcaactc taagcactta tctagaagac tgtaaatttg
     4381 acagagagcg aatagaactg ttttgcacgg aatatcagaa taataagaat tccctagaaa
     4441 tcctactggg aagtataggc agatctctcc ctcatataac ggatgtttct tggcgcttgg
     4501 aatatcagat aaagaccaat caacttcata ggatgtacag acctgcatat ttggtgacct
     4561 taagtgtaca gaacactgat tccccatcct atccagagat tagttttagt tgcagcatgg
     4621 aacaattaca ggacttggtg gggaaactta aagatgcttc gaaaagcctg gaaagagcaa
     4681 ctcagttgta agcggccgcc ggtaagccta tccctaaccc tctcctcggt ctcgattcta
     4741 cgtaagaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     4801 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     4861 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     4921 acagcagaga tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc
     4981 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     5041 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     5101 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     5161 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     5221 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     5281 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     5341 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     5401 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     5461 cagggggatc tgatccacca ccatgaagaa gcccgaactc accgctacca gcgttgaaaa
     5521 atttctcatc gagaagttcg acagtgtgag cgacctgatg cagttgtcgg agggcgaaga
     5581 gagccgagcc ttcagcttcg atgtcggcgg acgcggctat gtactgcggg tgaatagctg
     5641 cgctgatggc ttctacaaag accgctacgt gtaccgccac ttcgccagcg ctgcactacc
     5701 catccccgaa gtgttggaca tcggcgagtt cagcgagagc ctgacatact gcatcagtag
     5761 acgcgcccaa ggcgttactc tccaagacct ccccgaaaca gagctgcctg ctgtgttaca
     5821 gcctgtcgcc gaagctatgg atgctattgc cgccgccgac ctcagtcaaa ccagcggctt
     5881 cggcccattc gggccccaag gcatcggcca gtacacaacc tggcgggatt tcatttgcgc
     5941 cattgctgat ccccatgtct accactggca gaccgtgatg gacgacaccg tgtccgccag
     6001 cgtagctcaa gccctggacg aactgatgct gtgggccgaa gactgtcccg aggtgcgcca
     6061 cctcgtccat gccgacttcg gcagcaacaa cgtcctgacc gacaacggcc gcatcaccgc
     6121 cgtaatcgac tggtccgaag ctatgttcgg ggacagtcag tacgaggtgg ccaacatctt
     6181 cttctggcgg ccctggctgg cttgcatgga gcagcagact cgctacttcg agcgccggca
     6241 tcccgagctg gccggcagcc ctcgtctgcg agcctacatg ctgcgcatcg gcctggatca
     6301 gctctaccag agcctcgtgg acggcaactt cgacgatgct gcctgggctc aaggccgctg
     6361 cgatgccatc gtccgcagcg gggccggcac cgtcggtcgc acacaaatcg ctcgccggag
     6421 cgcagccgta tggaccgacg gctgcgtcga ggtgctggcc gacagcggca accgccggcc
     6481 cagtacacga ccgcgcgcta aggaggtagg tcgagtttaa gtacctttaa gaccaatgac
     6541 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6601 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     6661 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6721 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6781 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6841 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     6901 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6961 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7021 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7081 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7141 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     7201 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     7261 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     7321 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     7381 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7441 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7501 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7561 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7621 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7681 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7741 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7801 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     7861 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7921 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7981 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8041 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     8101 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     8161 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8221 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8281 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8341 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8401 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8461 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8521 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8581 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8641 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8701 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8761 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8821 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8881 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8941 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9001 gatcaaagga tcttc
//
