LOCUS       sequence-393008-       13593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBac-U6-gRNA(doublesex)-3xp3-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13593)
  AUTHORS   Li M, Kandul NP, Sun R, Yang T, Benetta ED, Brogan DJ, Antoshechkin 
            I, Sanchez C HM, Zhan Y, DeBeaubien NA, Loh YM, Su MP, Montell C, 
            Marshall JM, Akbari OS
  TITLE     Targeting Sex Determination to Suppress Mosquito Populations.
  JOURNAL   bioRxiv. 2023 Nov 15:2023.04.18.537404. doi: 
            10.1101/2023.04.18.537404. Preprint.
  PUBMED    37131747
REFERENCE   2  (bases 1 to 13593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Nov 15:2023.04.18.537404. doi: 10.1101/2023.04.18.537404. 
            Preprint."
COMMENT     Sequence Label: OA-1067B
FEATURES             Location/Qualifiers
     source          1..13593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3013..3075
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      10724..10733
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(10863..10881)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     11131..11150
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     11325..11348
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    12282..12403
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12319..12338)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12373..12392
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   13071..13105
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(13286..13302)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13286..13302)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13299..13321)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13310..13326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13334..13340,13341..13358,13359..13364))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13379..13400
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13517..13534)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaaata taacaaaatt
     2341 gtgatcccac aaaatgaagt ggggcaaaat caaataatta atagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcc gtgactataa cacaagttga
     2461 tttaataatt ttagccaaca cgtcgggctg cgtgtttttt gccgacgcgt ctgtgtacac
     2521 gttgattaac tggtcgatta aactgttgaa ataatttaat ttttggttct tctttaaatc
     2581 tgtgatgaaa ttttttaaaa taactttaaa ttcttcattg gtaaaaaatg ccacgttttg
     2641 caacttgtga gggtctaata tgaggtcaaa ctcagtagga gttttatcca aaaaagaaaa
     2701 catgattacg tctgtacacg aacgcgtatt aacgcagagt gcaaagtata agagggttaa
     2761 aaaatatatt ttacgcacca tatacgcatc gggttgatat cgttaatatg gatcaatttg
     2821 aacagttgat taacgtgtct ctgctcaagt ctttgatcaa aacgcaaatc gacgaaaatg
     2881 tgtcggacaa tatcaagtcg atgagcgaaa aactaaaaag gctagaatac gacaatctca
     2941 cagacagcgt tgagatatac ggtattcacg acagcaggct gaataataaa aaaattagaa
     3001 actattattt aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgcg
     3061 taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata
     3121 gatattaagt tttattatat ttacacttac atactaataa taaattcaac aaacaattta
     3181 tttatgttta tttatttatt aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca
     3241 aaacttttaa acattctctc ttttacaaaa ataaacttat tttgtacttt aaaaacagtc
     3301 atgttgtatt ataaaataag taattagctt aacttataca taatagaaac aaattatact
     3361 tattagtcag tcagaaacaa ctttggcaca tatcaatatt atgctctcga caaataactt
     3421 ttttgcattt tttgcacgat gcatttgcct ttcgccttat tttagagggg cagtaagtac
     3481 agtaagtacg ttttttcatt actggctctt cagtactgtc atctgatgta ccaggcactt
     3541 catttggcaa aatattagag atattatcgc gcaaatatct cttcaaagta ggagcttcta
     3601 aacgcttacg cataaacgat gacgtcaggc tcatgtaaag gtttctcata aattttttgc
     3661 gactttgaac cttttctccc ttgctactga cattatggct gtatataata aaagaattta
     3721 tgcaggcaat gtttatcatt ccgtacaata atgccatagg ccacctattc gtcttcctac
     3781 tgcaggtcat cacagaacac atttggtcta gcgtgtccac tccgccttta gtttgattat
     3841 aatacataac catttgcggt ttaccggtac tttcgttgat agaagcatcc tcatcacaag
     3901 atgataataa gtataccatc ttagctggct tcggtttata tgagacgaga gtaaggggtc
     3961 cgtcaaaaca aaacatcgat gttcccactg gcctggagcg actgtttttc agtacttccg
     4021 gtatctcgcg tttgtttgat cgcacggttc ccacaatggt taattcgagc tcgcccgggc
     4081 tcctaggaga tcttcatcac aaacgaaact attataatcg tcttcagggg aacggtagca
     4141 tcatatctgt caccagtcag tcggattcct ttctttcggc ataaattatg ctcgggtgac
     4201 gagacgagtt gaaaatcccg taaattaaaa catataccac actcgccgtc ctagacgacg
     4261 acgacgacga cgattcccaa ggaattgaac ccttcatttg catttcacta ccgctctgag
     4321 agatgcaaag cgcgccagac agtcgatcga atctgcagac cagagagtca catcaagaat
     4381 gttgacattc caccagaaga ctctaattat tcttcgctcg actgggaaaa ctgttaacaa
     4441 ctgctcgagt gtggtcggag cggctcacag tgcgttttcc cgtagacaaa aatcaaattt
     4501 catggtattt ttcggcgata aacagtaagt taaagggaac ccgaatttag tgctatataa
     4561 tttaattcca ctagagtttg tatcctttga tagatacgcg tatttcgacc tcaactgcaa
     4621 ggccgtcgtg tactagactt gactaatcca gactggtctt ttagttatga cttctgtcca
     4681 catctccata cattcaacgc actgtgcggc tgtgctgtgc gactccgtcg agtcgaccaa
     4741 catagttgaa acaaattgaa tatttaattg atcgttatag gaatggtgtt agatgagtca
     4801 tcctttacag taagcacata cagtattata attgaagatc gtcggcagat aggtgtgtag
     4861 ggtagagtat cagcaataag ttgggacgtt tgactttttg taggtagaca aaaactaaac
     4921 tttttttcgc ttctctatgt gtgcccctgg gtagcgttcc gttccgattg gggtgcgaac
     4981 gaatgaaatc gcccatcgag ttgatacgtc catccatcgc tagaaccgcg ttcgctgtag
     5041 aagactatat aagagcagag gcaagagtag tgaaatagcg acatgcagcc attctggtct
     5101 tagagctatg ctggaaacag catagcaagt taagataagg ctagtccgtt atcaacttga
     5161 aaaagtggca ccgagtcggt gctttttttg gtggttgttc atcgattcct aatattgcag
     5221 tgaagcagtt tgttgtgcat ggcgaagata aggttagggc gaggaatgcc ttggaaataa
     5281 gcggcactgg gccgtatgaa taaaaagcgt tgaactttac tacatgtagc atctactcaa
     5341 aaacaaaatc cacaaagttt actacacttt tggtatgaat aaaaaccttt tcgaacatgt
     5401 agtacctact actttaagaa aattctattc aaagtacaga gtaatgctgc atgttaagaa
     5461 aattccattc agagtgactc ttgaaatcaa taaaatcatg catatatgcc aaatttgtca
     5521 cttattcacg tgacaaatta tgtgaaaata tgaatattac tacattcccg ctatgcatcg
     5581 cgtaatcgat catgaaattt atgttggtta tgtaacttta cagtattccc tttatgtgca
     5641 tgtgaatttt ataatgattt gacaacaagg attttcctta gaattttcga attttagcta
     5701 gaaatctgta tcctatgaat gaatcttgag ataacagcaa ttgtaacatt ataaagttcc
     5761 taacattttc aagattccag tattgagtcg cgatcgctta attaaccgcg gatatgtctt
     5821 gccgaagcac attaacccaa gccatttata tatcaagtac aagttgattt gattctagtg
     5881 atggactgac acgaatctat ggcactgttg cagagcttac aaacaggtac ccggagtcgc
     5941 ttcaattatt caatcatcca ttcaaaaact tccatctctt ccaattagcc aatattcaaa
     6001 ttggaatcca gccagccgac ggaggcagat ggtctacctg gcaactatag agtttccatg
     6061 tttccagact ttccctcccg gtaaacggag acaaaacgac agacgtaagt aggtacatat
     6121 gcataccgca cggacaaatc aaatttgtct ggcagctcca attagagtcg ttaaaaattt
     6181 aacgatgcgc taaataactt caagctattt gtctcgctgg attggcttcg agtggtaaga
     6241 tcctatcaaa tgccgaaaac aaaaaacttt cttcttaatt gttcgttctt caacacctct
     6301 ccatggtgat aacggatacg gtttcattgt cagcatccat cctccgaaaa atacattacg
     6361 ccttgaaata tgcaatcgca aacacggatc tgtttggaac atttatttta ctatgaagag
     6421 atgcgatagg taatatttat ttgagcgttt aagatactca ttgttctctc aaagaatgtc
     6481 attgaaagcc aacgaggtca aatcaaatat tataataaaa aggtcaaaga ggactaactt
     6541 aaagctctct ttatggatag gaaaaaatat tttcgcccat cgctagaact tttaccgttt
     6601 ccattgagta tataactaag atgaatgagg ctaattgatc tcacaagtag agctacacgg
     6661 tcttagagct atgctggaaa cagcatagca agttaagata aggctagtcc gttatcaact
     6721 tgaaaaagtg gcaccgagtc ggtgcttttt ttaagatgct tcggcaatta ataataagga
     6781 atggtaaagc attcttcaaa tgttactatg ttctcaaatt ttcaagaatt aggtattcaa
     6841 gaaattcttt caggcatatt ccataatctg cacaagcagc tactttagaa attattgccg
     6901 ggcttaaatt atatgtacaa catttcctga gtattttctg acaaaaattc tcacaaggga
     6961 aggtcacgat ggagttacac ggggttttta accggactat ttttgttaga ttctacatgt
     7021 cgaaatatga aaaaccccaa agcctttcgg gtgatggacc agtggtgtag ccaggagggg
     7081 ctctgaaagg ggtaattttg tacgtatatt atattattaa gctaattgga aatttgacaa
     7141 aaatattttt ttagtattta ttgtgatggt agagaacaaa attgacatta atgtacgttt
     7201 gaaatgtatt tgccatgcct ttggcgtact aacatggagt atggacacca aaaccgattt
     7261 cgctagcgtt taaactcatc acaaacgaaa ctattataat cgtcttcagg ggaacggtag
     7321 catcatatct gtcaccagtc agtcggattc ctttctttcg gcataaatta tgctcgggtg
     7381 acgagacgag ttgaaaatcc cgtaaattaa aacatatacc acactcgccg tcctagacga
     7441 cgacgacgac gacgattccc aaggaattga acccttcatt tgcatttcac taccgctctg
     7501 agagatgcaa agcgcgccag acagtcgatc gaatctgcag accagagagt cacatcaaga
     7561 atgttgacat tccaccagaa gactctaatt attcttcgct cgactgggaa aactgttaac
     7621 aactgctcga gtgtggtcgg agcggctcac agtgcgtttt cccgtagaca aaaatcaaat
     7681 ttcatggtat ttttcggcga taaacagtaa gttaaaggga acccgaattt agtgctatat
     7741 aatttaattc cactagagtt tgtatccttt gatagatacg cgtatttcga cctcaactgc
     7801 aaggccgtcg tgtactagac ttgactaatc cagactggtc ttttagttat gacttctgtc
     7861 cacatctcca tacattcaac gcactgtgcg gctgtgctgt gcgactccgt cgagtcgacc
     7921 aacatagttg aaacaaattg aatatttaat tgatcgttat aggaatggtg ttagatgagt
     7981 catcctttac agtaagcaca tacagtatta taattgaaga tcgtcggcag ataggtgtgt
     8041 agggtagagt atcagcaata agttgggacg tttgactttt tgtaggtaga caaaaactaa
     8101 actttttttc gcttctctat gtgtgcccct gggtagcgtt ccgttccgat tggggtgcga
     8161 acgaatgaaa tcgcccatcg agttgatacg tccatccatc gctagaaccg cgttcgctgt
     8221 agaagactat ataagagcag aggcaagagt agtgaaatgg tagctggccg ttgccaaagt
     8281 cttagagcta tgctggaaac agcatagcaa gttaagataa ggctagtccg ttatcaactt
     8341 gaaaaagtgg caccgagtcg gtgctttttt tggtggttgt tcatcgattc ctaatattgc
     8401 agtgaagcag tttgttgtgc atggcgaaga taaggttagg gcgaggaatg ccttggaaat
     8461 aagcggcact gggccgtatg aataaaaagc gttgaacttt actacatgta gcatctactc
     8521 aaaaacaaaa tccacaaagt ttactacact tttggtatga ataaaaacct tttcgaacat
     8581 gtagtaccta ctactttaag aaaattctat tcaaagtaca gagtaatgct gcatgttaag
     8641 aaaattccat tcagagtgac tcttgaaatc aataaaatca tgcatatatg ccaaatttgt
     8701 cacttattca cgtgacaaat tatgtgaaaa tatgaatatt actacattcc cgctatgcat
     8761 cgcgtaatcg atcatgaaat ttatgttggt tatgtaactt tacagtattc cctttatgtg
     8821 catgtgaatt ttataatgat ttgacaacaa ggattttcct tagaattttc gaattttagc
     8881 tagaaatctg tatcctatga atgaatcttg agataacagc aattgtaaca ttataaagtt
     8941 cctaacattt tcaagattcc agtattgagt cgcgatcgct taattaaccg cggatatgtc
     9001 ttgccgaagc acattaaccc aagccattta tatatcaagt acaagttgat ttgattctag
     9061 tgatggactg acacgaatct atggcactgt tgcagagctt acaaacaggt acccggagtc
     9121 gcttcaatta ttcaatcatc cattcaaaaa cttccatctc ttccaattag ccaatattca
     9181 aattggaatc cagccagccg acggaggcag atggtctacc tggcaactat agagtttcca
     9241 tgtttccaga ctttccctcc cggtaaacgg agacaaaacg acagacgtaa gtaggtacat
     9301 atgcataccg cacggacaaa tcaaatttgt ctggcagctc caattagagt cgttaaaaat
     9361 ttaacgatgc gctaaataac ttcaagctat ttgtctcgct ggattggctt cgagtggtaa
     9421 gatcctatca aatgccgaaa acaaaaaact ttcttcttaa ttgttcgttc ttcaacacct
     9481 ctccatggtg ataacggata cggtttcatt gtcagcatcc atcctccgaa aaatacatta
     9541 cgccttgaaa tatgcaatcg caaacacgga tctgtttgga acatttattt tactatgaag
     9601 agatgcgata ggtaatattt atttgagcgt ttaagatact cattgttctc tcaaagaatg
     9661 tcattgaaag ccaacgaggt caaatcaaat attataataa aaaggtcaaa gaggactaac
     9721 ttaaagctct ctttatggat aggaaaaaat attttcgccc atcgctagaa cttttaccgt
     9781 ttccattgag tatataacta agatgaatga ggctaattga tctgtcgtcg tttttttccg
     9841 ggtcttagag ctatgctgga aacagcatag caagttaaga taaggctagt ccgttatcaa
     9901 cttgaaaaag tggcaccgag tcggtgcttt ttttaagatg cttcggcaat taataataag
     9961 gaatggtaaa gcattcttca aatgttacta tgttctcaaa ttttcaagaa ttaggtattc
    10021 aagaaattct ttcaggcata ttccataatc tgcacaagca gctactttag aaattattgc
    10081 cgggcttaaa ttatatgtac aacatttcct gagtattttc tgacaaaaat tctcacaagg
    10141 gaaggtcacg atggagttac acggggtttt taaccggact atttttgtta gattctacat
    10201 gtcgaaatat gaaaaacccc aaagcctttc gggtgatgga ccagtggtgt agccaggagg
    10261 ggctctgaaa ggggtaattt tgtacgtata ttatattatt aagctaattg gaaatttgac
    10321 aaaaatattt ttttagtatt tattgtgatg gtagagaaca aaattgacat taatgtacgt
    10381 ttgaaatgta tttgccatgc ctttggcgta ctaacatgga gtatggacac caaaaccgat
    10441 ttcggccttt tgggcccggc gcgccaattc gagctcgccc ggggatctaa ttcaattaga
    10501 gactaattca attagagcta attcaattag gatccaagct tatcgatttc gaaccctcga
    10561 ccgccggagt ataaatagag gcgcttcgtc tacggagcga caattcaatt caaacaagca
    10621 aagtgaacac gtcgctaagc gaaagctaag caaataaaca agcgcagctg aacaagctaa
    10681 acaatcgggg taccgctaga gtcgacggta cgatccaccg gtcgccacca tggtgagcaa
    10741 gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa
    10801 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
    10861 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
    10921 ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa
    10981 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
    11041 tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa
    11101 gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg
    11161 ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca
    11221 ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc
    11281 caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc
    11341 ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct
    11401 gttcctgggg catggcaccg gcagcaccgg cagcggcagc tccggcaccg cctcctccga
    11461 ggacaacaac atggccgtta tcaaggaatt tatgcgcttc aaagttagga tggagggatc
    11521 catgaacgga catgagttcg agatcgaggg agagggcgag ggacgcccgt atgaaggcac
    11581 acaaacagcc aaactcaagg tcaccaaggg cggaccactg cccttcgcct gggatatcct
    11641 gagtccccag tttatgtacg gcagcaaggc ctacgttaag caccccgctg acataccgga
    11701 ctacaaaaag ctgtcctttc cggaaggctt caagtgggag cgcgtgatga atttcgaaga
    11761 cggaggactg gtcactgtga cccaagatag cagtttgcag gacggtacac tgatctataa
    11821 ggttaaaatg cgcggcacta actttccgcc agatggccca gtgatgcaga agaagaccat
    11881 gggttgggag gcatccaccg aacgtctgta ccctcgagac ggagtgctca agggcgagat
    11941 ccatcaggcc ctcaaactga aagatggtgg tcactacctg gtcgaattta agaccattta
    12001 catggccaag aagccggttc agctgcccgg atattattat gtggatacga aactggatat
    12061 aacttcgcat aacgaagact acaccattgt cgagcagtat gagcgcagcg aaggccgaca
    12121 tcacctgttc ctctacggca tggacgagct gtacaagtag gcggccgcga ctctagatca
    12181 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
    12241 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
    12301 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    12361 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaagcttc taggccggcc
    12421 gatctcggat ctgacaatgt tcagtgcaga gactcggcta cgcctcgtgg actttgaagt
    12481 tgaccaacaa tgtttattct tacctctaat agtcctctgt ggcaaggtca agattctgtt
    12541 agaagccaat gaagaacctg gttgttcaat aacattttgt tcgtctaata tttcactacc
    12601 gcttgacgtt ggctgcactt catgtacctc atctataaac gcttcttctg tatcgctctg
    12661 gacgtcatct tcacttacgt gatctgatat ttcactgtca gaatcctcac caacaagctc
    12721 gtcatcgctt tgcagaagag cagagaggat atgctcatcg tctaaagaac tacccatttt
    12781 attatatatt agtcacgata tctataacaa gaaaatatat atataataag ttatcacgta
    12841 agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa
    12901 agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta
    12961 ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca
    13021 cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa
    13081 ttttacgcag actatctttc tagggttaaa aaagatttgc gctttactcg acctaaactt
    13141 taaacacgtc atagaatctt cgtttgacaa aaaccacatt gtggccaagc tgtgtgacgc
    13201 gacgcgcgct aaagaatggc aaaccaagtc gcgcgagcgt cgactctaga ggatccccgg
    13261 gtaccgagct cgaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc
    13321 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    13381 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    13441 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    13501 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    13561 cggtatcagc tcactcaaag gcggtaatac ggt
//
