LOCUS       sequence-393011-       10411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBac-U6-gRNA(Intersex)-3xp3-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10411)
  AUTHORS   Li M, Kandul NP, Sun R, Yang T, Benetta ED, Brogan DJ, Antoshechkin 
            I, Sanchez C HM, Zhan Y, DeBeaubien NA, Loh YM, Su MP, Montell C, 
            Marshall JM, Akbari OS
  TITLE     Targeting Sex Determination to Suppress Mosquito Populations.
  JOURNAL   bioRxiv. 2023 Nov 15:2023.04.18.537404. doi: 
            10.1101/2023.04.18.537404. Preprint.
  PUBMED    37131747
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Nov 15:2023.04.18.537404. doi: 10.1101/2023.04.18.537404. 
            Preprint."
COMMENT     Sequence Label: OA-1055J
FEATURES             Location/Qualifiers
     source          1..10411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3013..3075
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      7542..7551
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7681..7699)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7949..7968
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8143..8166
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    9100..9221
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9137..9156)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9191..9210
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   9889..9923
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10104..10120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10104..10120)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10117..10139)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10128..10144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10152..10158,10159..10176,10177..10182))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10197..10218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10335..10352)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaaata taacaaaatt
     2341 gtgatcccac aaaatgaagt ggggcaaaat caaataatta atagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcc gtgactataa cacaagttga
     2461 tttaataatt ttagccaaca cgtcgggctg cgtgtttttt gccgacgcgt ctgtgtacac
     2521 gttgattaac tggtcgatta aactgttgaa ataatttaat ttttggttct tctttaaatc
     2581 tgtgatgaaa ttttttaaaa taactttaaa ttcttcattg gtaaaaaatg ccacgttttg
     2641 caacttgtga gggtctaata tgaggtcaaa ctcagtagga gttttatcca aaaaagaaaa
     2701 catgattacg tctgtacacg aacgcgtatt aacgcagagt gcaaagtata agagggttaa
     2761 aaaatatatt ttacgcacca tatacgcatc gggttgatat cgttaatatg gatcaatttg
     2821 aacagttgat taacgtgtct ctgctcaagt ctttgatcaa aacgcaaatc gacgaaaatg
     2881 tgtcggacaa tatcaagtcg atgagcgaaa aactaaaaag gctagaatac gacaatctca
     2941 cagacagcgt tgagatatac ggtattcacg acagcaggct gaataataaa aaaattagaa
     3001 actattattt aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgcg
     3061 taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata
     3121 gatattaagt tttattatat ttacacttac atactaataa taaattcaac aaacaattta
     3181 tttatgttta tttatttatt aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca
     3241 aaacttttaa acattctctc ttttacaaaa ataaacttat tttgtacttt aaaaacagtc
     3301 atgttgtatt ataaaataag taattagctt aacttataca taatagaaac aaattatact
     3361 tattagtcag tcagaaacaa ctttggcaca tatcaatatt atgctctcga caaataactt
     3421 ttttgcattt tttgcacgat gcatttgcct ttcgccttat tttagagggg cagtaagtac
     3481 agtaagtacg ttttttcatt actggctctt cagtactgtc atctgatgta ccaggcactt
     3541 catttggcaa aatattagag atattatcgc gcaaatatct cttcaaagta ggagcttcta
     3601 aacgcttacg cataaacgat gacgtcaggc tcatgtaaag gtttctcata aattttttgc
     3661 gactttgaac cttttctccc ttgctactga cattatggct gtatataata aaagaattta
     3721 tgcaggcaat gtttatcatt ccgtacaata atgccatagg ccacctattc gtcttcctac
     3781 tgcaggtcat cacagaacac atttggtcta gcgtgtccac tccgccttta gtttgattat
     3841 aatacataac catttgcggt ttaccggtac tttcgttgat agaagcatcc tcatcacaag
     3901 atgataataa gtataccatc ttagctggct tcggtttata tgagacgaga gtaaggggtc
     3961 cgtcaaaaca aaacatcgat gttcccactg gcctggagcg actgtttttc agtacttccg
     4021 gtatctcgcg tttgtttgat cgcacggttc ccacaatggt taattcgagc tcgcccgggc
     4081 tcctaggaga tcttcatcac aaacgaaact attataatcg tcttcagggg aacggtagca
     4141 tcatatctgt caccagtcag tcggattcct ttctttcggc ataaattatg ctcgggtgac
     4201 gagacgagtt gaaaatcccg taaattaaaa catataccac actcgccgtc ctagacgacg
     4261 acgacgacga cgattcccaa ggaattgaac ccttcatttg catttcacta ccgctctgag
     4321 agatgcaaag cgcgccagac agtcgatcga atctgcagac cagagagtca catcaagaat
     4381 gttgacattc caccagaaga ctctaattat tcttcgctcg actgggaaaa ctgttaacaa
     4441 ctgctcgagt gtggtcggag cggctcacag tgcgttttcc cgtagacaaa aatcaaattt
     4501 catggtattt ttcggcgata aacagtaagt taaagggaac ccgaatttag tgctatataa
     4561 tttaattcca ctagagtttg tatcctttga tagatacgcg tatttcgacc tcaactgcaa
     4621 ggccgtcgtg tactagactt gactaatcca gactggtctt ttagttatga cttctgtcca
     4681 catctccata cattcaacgc actgtgcggc tgtgctgtgc gactccgtcg agtcgaccaa
     4741 catagttgaa acaaattgaa tatttaattg atcgttatag gaatggtgtt agatgagtca
     4801 tcctttacag taagcacata cagtattata attgaagatc gtcggcagat aggtgtgtag
     4861 ggtagagtat cagcaataag ttgggacgtt tgactttttg taggtagaca aaaactaaac
     4921 tttttttcgc ttctctatgt gtgcccctgg gtagcgttcc gttccgattg gggtgcgaac
     4981 gaatgaaatc gcccatcgag ttgatacgtc catccatcgc tagaaccgcg ttcgctgtag
     5041 aagactatat aagagcagag gcaagagtag tgaaatgcgg gattcgctct cgacgagtct
     5101 tagagctatg ctggaaacag catagcaagt taagataagg ctagtccgtt atcaacttga
     5161 aaaagtggca ccgagtcggt gctttttttg gtggttgttc atcgattcct aatattgcag
     5221 tgaagcagtt tgttgtgcat ggcgaagata aggttagggc gaggaatgcc ttggaaataa
     5281 gcggcactgg gccgtatgaa taaaaagcgt tgaactttac tacatgtagc atctactcaa
     5341 aaacaaaatc cacaaagttt actacacttt tggtatgaat aaaaaccttt tcgaacatgt
     5401 agtacctact actttaagaa aattctattc aaagtacaga gtaatgctgc atgttaagaa
     5461 aattccattc agagtgactc ttgaaatcaa taaaatcatg catatatgcc aaatttgtca
     5521 cttattcacg tgacaaatta tgtgaaaata tgaatattac tacattcccg ctatgcatcg
     5581 cgtaatcgat catgaaattt atgttggtta tgtaacttta cagtattccc tttatgtgca
     5641 tgtgaatttt ataatgattt gacaacaagg attttcctta gaattttcga attttagcta
     5701 gaaatctgta tcctatgaat gaatcttgag ataacagcaa ttgtaacatt ataaagttcc
     5761 taacattttc aagattccag tattgagtcg cgatcgctta attaaccgcg gatatgtctt
     5821 gccgaagcac attaacccaa gccatttata tatcaagtac aagttgattt gattctagtg
     5881 atggactgac acgaatctat ggcactgttg cagagcttac aaacaggtac ccggagtcgc
     5941 ttcaattatt caatcatcca ttcaaaaact tccatctctt ccaattagcc aatattcaaa
     6001 ttggaatcca gccagccgac ggaggcagat ggtctacctg gcaactatag agtttccatg
     6061 tttccagact ttccctcccg gtaaacggag acaaaacgac agacgtaagt aggtacatat
     6121 gcataccgca cggacaaatc aaatttgtct ggcagctcca attagagtcg ttaaaaattt
     6181 aacgatgcgc taaataactt caagctattt gtctcgctgg attggcttcg agtggtaaga
     6241 tcctatcaaa tgccgaaaac aaaaaacttt cttcttaatt gttcgttctt caacacctct
     6301 ccatggtgat aacggatacg gtttcattgt cagcatccat cctccgaaaa atacattacg
     6361 ccttgaaata tgcaatcgca aacacggatc tgtttggaac atttatttta ctatgaagag
     6421 atgcgatagg taatatttat ttgagcgttt aagatactca ttgttctctc aaagaatgtc
     6481 attgaaagcc aacgaggtca aatcaaatat tataataaaa aggtcaaaga ggactaactt
     6541 aaagctctct ttatggatag gaaaaaatat tttcgcccat cgctagaact tttaccgttt
     6601 ccattgagta tataactaag atgaatgagg ctaattgatg gacaacattt ccaaggttag
     6661 tcttagagct atgctggaaa cagcatagca agttaagata aggctagtcc gttatcaact
     6721 tgaaaaagtg gcaccgagtc ggtgcttttt ttaagatgct tcggcaatta ataataagga
     6781 atggtaaagc attcttcaaa tgttactatg ttctcaaatt ttcaagaatt aggtattcaa
     6841 gaaattcttt caggcatatt ccataatctg cacaagcagc tactttagaa attattgccg
     6901 ggcttaaatt atatgtacaa catttcctga gtattttctg acaaaaattc tcacaaggga
     6961 aggtcacgat ggagttacac ggggttttta accggactat ttttgttaga ttctacatgt
     7021 cgaaatatga aaaaccccaa agcctttcgg gtgatggacc agtggtgtag ccaggagggg
     7081 ctctgaaagg ggtaattttg tacgtatatt atattattaa gctaattgga aatttgacaa
     7141 aaatattttt ttagtattta ttgtgatggt agagaacaaa attgacatta atgtacgttt
     7201 gaaatgtatt tgccatgcct ttggcgtact aacatggagt atggacacca aaaccgattt
     7261 cggccttttg ggcccggcgc gccaattcga gctcgcccgg ggatctaatt caattagaga
     7321 ctaattcaat tagagctaat tcaattagga tccaagctta tcgatttcga accctcgacc
     7381 gccggagtat aaatagaggc gcttcgtcta cggagcgaca attcaattca aacaagcaaa
     7441 gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagctgaa caagctaaac
     7501 aatcggggta ccgctagagt cgacggtacg atccaccggt cgccaccatg gtgagcaagg
     7561 gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg
     7621 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     7681 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc
     7741 agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga
     7801 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc
     7861 tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga
     7921 tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg
     7981 aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg
     8041 ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca
     8101 agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac atcacctccc
     8161 acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt
     8221 tcctggggca tggcaccggc agcaccggca gcggcagctc cggcaccgcc tcctccgagg
     8281 acaacaacat ggccgttatc aaggaattta tgcgcttcaa agttaggatg gagggatcca
     8341 tgaacggaca tgagttcgag atcgagggag agggcgaggg acgcccgtat gaaggcacac
     8401 aaacagccaa actcaaggtc accaagggcg gaccactgcc cttcgcctgg gatatcctga
     8461 gtccccagtt tatgtacggc agcaaggcct acgttaagca ccccgctgac ataccggact
     8521 acaaaaagct gtcctttccg gaaggcttca agtgggagcg cgtgatgaat ttcgaagacg
     8581 gaggactggt cactgtgacc caagatagca gtttgcagga cggtacactg atctataagg
     8641 ttaaaatgcg cggcactaac tttccgccag atggcccagt gatgcagaag aagaccatgg
     8701 gttgggaggc atccaccgaa cgtctgtacc ctcgagacgg agtgctcaag ggcgagatcc
     8761 atcaggccct caaactgaaa gatggtggtc actacctggt cgaatttaag accatttaca
     8821 tggccaagaa gccggttcag ctgcccggat attattatgt ggatacgaaa ctggatataa
     8881 cttcgcataa cgaagactac accattgtcg agcagtatga gcgcagcgaa ggccgacatc
     8941 acctgttcct ctacggcatg gacgagctgt acaagtaggc ggccgcgact ctagatcata
     9001 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     9061 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     9121 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     9181 cattctagtt gtggtttgtc caaactcatc aatgtatctt aaagcttcta ggccggccga
     9241 tctcggatct gacaatgttc agtgcagaga ctcggctacg cctcgtggac tttgaagttg
     9301 accaacaatg tttattctta cctctaatag tcctctgtgg caaggtcaag attctgttag
     9361 aagccaatga agaacctggt tgttcaataa cattttgttc gtctaatatt tcactaccgc
     9421 ttgacgttgg ctgcacttca tgtacctcat ctataaacgc ttcttctgta tcgctctgga
     9481 cgtcatcttc acttacgtga tctgatattt cactgtcaga atcctcacca acaagctcgt
     9541 catcgctttg cagaagagca gagaggatat gctcatcgtc taaagaacta cccattttat
     9601 tatatattag tcacgatatc tataacaaga aaatatatat ataataagtt atcacgtaag
     9661 tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag
     9721 ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc
     9781 gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca
     9841 gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt
     9901 ttacgcagac tatctttcta gggttaaaaa agatttgcgc tttactcgac ctaaacttta
     9961 aacacgtcat agaatcttcg tttgacaaaa accacattgt ggccaagctg tgtgacgcga
    10021 cgcgcgctaa agaatggcaa accaagtcgc gcgagcgtcg actctagagg atccccgggt
    10081 accgagctcg aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
    10141 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    10201 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    10261 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    10321 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    10381 gtatcagctc actcaaaggc ggtaatacgg t
//
