LOCUS       sequence-393015-        9841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PTPRD-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..9841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             7183..7227
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7228..7245
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7385..7402)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7391..7502
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7620..7640)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7625..7954
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7805..7940
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7867..7886
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8002..8049
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8068..8667
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8068..8087)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8564..8584
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8797..8918
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8834..8853)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8888..8907
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8971..9762,9763..9831))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9594..9613
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9832..9841,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccga aacacctcct
     2761 agattcacca gaacacccgt ggaccagaca ggtgttagtg gcggagtggc cagcttcatc
     2821 tgtcaagcca caggcgaccc cagacctaag atcgtgtgga acaagaaagg caagaaggtg
     2881 tccaaccagc gcttcgaagt gatcgagttc gatgatggca gcggcagcgt gctgagaatc
     2941 cagcctctga gaacacccag agatgaggcc atctacgagt gcgtggccag caacaatgtg
     3001 ggcgagatca gcgtgtccac cagactgaca gtgctgagag aggatcagat ccccagaggc
     3061 ttccccacca tcgatatggg acctcagctg aaggtggtgg aaagaaccag aaccgccaca
     3121 atgctgtgtg ccgcctctgg caatcctgat cctgagatta cctggttcaa ggacttcctg
     3181 cctgtggaca cctccaacaa caacggccgg atcaagcagc tgagaagcga gtctatcggc
     3241 ggcactccta ttagaggcgc cctgcagatt gagcagagcg aggaaagcga ccagggcaaa
     3301 tatgagtgtg tggccaccaa tagcgccggc acaagatact ctgcccctgc caatctgtac
     3361 gtgcgcgaac tgagagaagt gcggagagtg cctcctcggt ttagcatccc tcctaccaac
     3421 cacgagatca tgcctggcgg ctccgtgaat atcacatgtg tggccgtggg cagccccatg
     3481 ccttatgtga aatggatgct gggcgccgag gatctgaccc ctgaggatga tatgcccatc
     3541 ggcagaaacg tgctggaact gaacgatgtg cggcagagcg ccaattacac ctgtgtggct
     3601 atgagcaccc tgggcgtgat cgaggccatt gctcagatca cagtgaaggc cctgcctaag
     3661 cctcctggca cacctgtggt tacagagagc acagccacca gcatcaccct gacctgggat
     3721 agcggaaatc ccgagcctgt gtcctactac atcatccagc acaagcccaa gaacagcgag
     3781 gaactgtaca aagaaatcga cggcgtggcc actaccagat acagtgtggc cggactgagc
     3841 ccctacagcg actacgagtt tagagtggtg gccgtgaaca acatcggcag gggtcctcca
     3901 tctgagcctg tgctgacaca gacatctgag caggccccat ctagcgcccc tagagatgtt
     3961 caggccagaa tgctgagcag caccaccatc ctggtgcagt ggaaagagcc tgaggaaccc
     4021 aacggccaga tccagggcta cagagtgtac tacacaatgg accccacaca gcacgtcaac
     4081 aactggatga agcacaacgt ggccgacagc cagatcacca caatcggcaa cctggtgcct
     4141 cagaaaacct actccgtgaa ggtgctggcc ttcacctcta tcggagatgg ccctctgagc
     4201 agcgacatcc aagtgatcac acagactggc gtgcccggcc agccactgaa tttcaaggcc
     4261 gagcctgaga gcgaaaccag catcctgctt agctggaccc ctcctagaag cgacacaatc
     4321 gccaactacg agctggtgta caaggatggc gagcacggcg aggaacagag aatcacaatc
     4381 gagcccggca ccagctacag actgcagggc ctgaagccta acagcctgta ctacttcaga
     4441 ctggccgcca gatctcctca aggcctggga gcctctacag ccgagatctc tgccagaacc
     4501 atgcagagca aacccagcgc tcctccacag gacatcagct gtacaagccc cagctctacc
     4561 tctatcctgg tgtcttggca gcctcctcca gtggaaaagc agaacggcat catcaccgag
     4621 tacagcatca agtacacagc cgtggacggc gaagatgaca agcctcacga gatcctgggc
     4681 atccccagcg ataccaccaa gtacctgctg gaacagctgg aaaagtggac cgagtaccgg
     4741 atcaccgtga cagcccacac agatgttgga cctggacctg agagcctgtc cgtgctgatc
     4801 agaacaaacg aggatgtgcc tagcggccct cctcggaaag tggaagtgga agccgtgaac
     4861 tccaccagcg tgaaagtgtc ttggagaagc cccgtgccta acaagcagca tggccagatc
     4921 agaggatacc aggtgcacta cgtgcggatg gaaaacggcg agcctaaggg ccagcctatg
     4981 ctgaaggatg tcatgctggc cgacgctcag tgggagtttg atgacaccac cgagcacgac
     5041 atgatcatca gcggactgca gcccgagaca agctactctc tgacagtgac cgcctacacc
     5101 acaaaaggcg acggcgctag aagcaagcct aagctggttt ctacaactgg cgccgtgcct
     5161 ggcaagccca gactggttat caatcacacc cagatgaaca cagccctgat ccagtggcac
     5221 ccacctgtgg atacctttgg acctctgcag ggatacagac tgaagttcgg ccggaaggac
     5281 atggaacccc tgaccacact ggaattcagc gagaaagagg accacttcac cgccaccgat
     5341 attcacaagg gcgccagcta cgtgttccgg ctgagcgcca gaaacaaagt cggcttcggc
     5401 gaagagatgg tcaaagagat cagcatcccc gaagaggtgc ccaccggctt tccacagaat
     5461 ctgcacagcg agggcaccac ctctacaagc gttcagctct cttggcaacc accagtgctg
     5521 gctgagcgga atggaatcat cacaaagtac accctgctgt accgggacat caacatccct
     5581 ctgctgccca tggaacaact gatcgtgccc gccgatacca caatgaccct gaccggactg
     5641 aagcctgata ccacctacga tgtgaaagtg cgggcccaca caagcaaagg ccctggacct
     5701 tatagcccca gcgtgcagtt tagaaccctg ccagttgacc aggtgttcgc caagaacttc
     5761 cacgtgaagg ccgtgatgaa gacctccgtg ctgctgtctt gggagatccc cgagaactac
     5821 aacagcgcca tgccattcaa gatcctgtac gacgacggca agatggtgga agaggtggac
     5881 ggcagagcca ctcagaaact gattgtgaat ctgaagcccg agaagtccta cagcttcgtg
     5941 ctgaccaaca gaggcaattc tgctggcggc ctgcagcaca gagtgacagc caaaacagct
     6001 cccgacgtgc tgaggaccaa gcctgccttt atcggcaaga ccaacctgga cggcatgatt
     6061 accgtgcagc tgcctgaagt gcccgccaac gagaacatca agggctacta tatcatcatc
     6121 gtgcccctga agaagtcccg gggcaagttc atcaagcctt gggagagccc tgacgagatg
     6181 gaactggacg agctgctgaa agagatctcc cggaagcgga gatccatcag atatggcaga
     6241 gaggtcgagc tgaagcccta tatcgccgct catttcgatg tgctgcccac cgagtttaca
     6301 ctgggcgacg ataagcacta cggcggcttc accaacaaac agctgcagag cggccaagag
     6361 tacgtgttct ttgtgctggc agtgatggaa cacgccgaga gcaagatgta cgccacctct
     6421 ccatacagcg accccgtggt gtctatggat ctggaccctc agcctatcac cgacgaagag
     6481 gaaggcgcgc cgggcggcga caagacccac acctgccccc cctgcccagc tccagaactg
     6541 ctgggagggc cttccgtgtt cctgttcccc cccaagccca aggacaccct gatgatcagc
     6601 aggacccccg aggtgacctg cgtggtggtg gccgtgtccc acgaggaccc agaggtgaag
     6661 ttcaactggt acgtggacgg cgtggaggtg cacaacgcca agaccaagcc cagagaggag
     6721 cagtacaaca gcacctacag ggtggtgtcc gtgctgaccg tgctgcacca ggactggctg
     6781 aacggcaaag aatacaagtg caaagtctcc aacaaggccc tggctgcccc aatcgaaaag
     6841 acaatcagca aggccaaggg ccagccacgg gagccccagg tgtacaccct gccccccagc
     6901 cgggaggaga tgaccaagaa ccaggtgtcc ctgacctgtc tggtgaaggg cttctacccc
     6961 agcgatatcg ccgtggagtg ggagagcaac ggccagcccg agaacaacta caagaccacc
     7021 cccccagtgc tggacagcga cggcagcttc ttcctgtaca gcaagctgac cgtggacaag
     7081 tccaggtggc agcagggcaa cgtgttcagc tgcagcgtga tgcacgaggc cctgcacaac
     7141 cactacaccc agaagtccct gagcctgagc cccggcggca gcggtcttaa tgatattttc
     7201 gaggctcaga agattgagtg gcatgaacac caccaccatc accactaagg ccggcctgta
     7261 gtcactggca caacgcgtta cccggtaagc caatcgggta tacacggtcg tcatactgca
     7321 gacagggttc ttctactttg caagatagtc ttgagtagta aaataaatag atagagaaaa
     7381 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     7441 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     7501 tcacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     7561 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     7621 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7681 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     7741 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     7801 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7861 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     7921 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     7981 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     8041 taatacgaca aggtgaggaa ctaaaccatg accgagtaca agcccacggt gcgcctcgcc
     8101 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     8161 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     8221 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     8281 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     8341 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     8401 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     8461 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     8521 gagcgcgccg gggtgcccgc cttcctggag acatccgcgc cccgcaacct ccccttctac
     8581 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     8641 tgcatgaccc gcaagcccgg tgcctgacac gtgctacgag atttcgattc caccgccgcc
     8701 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     8761 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     8821 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8881 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     8941 tctagctaga gcttggcgta atcatggtca ttaccaatgc ttaatcagtg aggcacctat
     9001 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9061 tacgatacgg gagggcttac catctggccc cagcgctgcg atgataccgc gagaaccacg
     9121 ctcaccggct ccggatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9181 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9241 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc atcgctacag gcatcgtggt
     9301 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9361 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9421 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9481 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9541 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9601 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9661 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9721 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9781 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tattcttcct
     9841 t
//
