LOCUS       sequence-393016-       17021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBac-U6-gRNA(doublesex+Intersex+bTublin)-3xp3-tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17021)
  AUTHORS   Li M, Kandul NP, Sun R, Yang T, Benetta ED, Brogan DJ, Antoshechkin 
            I, Sanchez C HM, Zhan Y, DeBeaubien NA, Loh YM, Su MP, Montell C, 
            Marshall JM, Akbari OS
  TITLE     Targeting Sex Determination to Suppress Mosquito Populations.
  JOURNAL   bioRxiv. 2023 Nov 15:2023.04.18.537404. doi: 
            10.1101/2023.04.18.537404. Preprint.
  PUBMED    37131747
REFERENCE   2  (bases 1 to 17021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Nov 15:2023.04.18.537404. doi: 10.1101/2023.04.18.537404. 
            Preprint."
COMMENT     Sequence Label: OA-1067L
FEATURES             Location/Qualifiers
     source          1..17021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3013..3075
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     regulatory      10981..10990
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(11120..11138)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     11388..11407
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     11582..11605
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    12539..12660
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12576..12595)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12630..12649
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   16499..16533
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(16714..16730)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16714..16730)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(16727..16749)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    16738..16754
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16762..16768,16769..16786,16787..16792))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16807..16828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(16945..16962)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaaata taacaaaatt
     2341 gtgatcccac aaaatgaagt ggggcaaaat caaataatta atagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcc gtgactataa cacaagttga
     2461 tttaataatt ttagccaaca cgtcgggctg cgtgtttttt gccgacgcgt ctgtgtacac
     2521 gttgattaac tggtcgatta aactgttgaa ataatttaat ttttggttct tctttaaatc
     2581 tgtgatgaaa ttttttaaaa taactttaaa ttcttcattg gtaaaaaatg ccacgttttg
     2641 caacttgtga gggtctaata tgaggtcaaa ctcagtagga gttttatcca aaaaagaaaa
     2701 catgattacg tctgtacacg aacgcgtatt aacgcagagt gcaaagtata agagggttaa
     2761 aaaatatatt ttacgcacca tatacgcatc gggttgatat cgttaatatg gatcaatttg
     2821 aacagttgat taacgtgtct ctgctcaagt ctttgatcaa aacgcaaatc gacgaaaatg
     2881 tgtcggacaa tatcaagtcg atgagcgaaa aactaaaaag gctagaatac gacaatctca
     2941 cagacagcgt tgagatatac ggtattcacg acagcaggct gaataataaa aaaattagaa
     3001 actattattt aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgcg
     3061 taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata
     3121 gatattaagt tttattatat ttacacttac atactaataa taaattcaac aaacaattta
     3181 tttatgttta tttatttatt aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca
     3241 aaacttttaa acattctctc ttttacaaaa ataaacttat tttgtacttt aaaaacagtc
     3301 atgttgtatt ataaaataag taattagctt aacttataca taatagaaac aaattatact
     3361 tattagtcag tcagaaacaa ctttggcaca tatcaatatt atgctctcga caaataactt
     3421 ttttgcattt tttgcacgat gcatttgcct ttcgccttat tttagagggg cagtaagtac
     3481 agtaagtacg ttttttcatt actggctctt cagtactgtc atctgatgta ccaggcactt
     3541 catttggcaa aatattagag atattatcgc gcaaatatct cttcaaagta ggagcttcta
     3601 aacgcttacg cataaacgat gacgtcaggc tcatgtaaag gtttctcata aattttttgc
     3661 gactttgaac cttttctccc ttgctactga cattatggct gtatataata aaagaattta
     3721 tgcaggcaat gtttatcatt ccgtacaata atgccatagg ccacctattc gtcttcctac
     3781 tgcaggtcat cacagaacac atttggtcta gcgtgtccac tccgccttta gtttgattat
     3841 aatacataac catttgcggt ttaccggtac tttcgttgat agaagcatcc tcatcacaag
     3901 atgataataa gtataccatc ttagctggct tcggtttata tgagacgaga gtaaggggtc
     3961 cgtcaaaaca aaacatcgat gttcccactg gcctggagcg actgtttttc agtacttccg
     4021 gtatctcgcg tttgtttgat cgcacggttc ccacaatggt taattcgagc tcgcccgggc
     4081 tcctaggaga tcttcatcac aaacgaaact attataatcg tcttcagggg aacggtagca
     4141 tcatatctgt caccagtcag tcggattcct ttctttcggc ataaattatg ctcgggtgac
     4201 gagacgagtt gaaaatcccg taaattaaaa catataccac actcgccgtc ctagacgacg
     4261 acgacgacga cgattcccaa ggaattgaac ccttcatttg catttcacta ccgctctgag
     4321 agatgcaaag cgcgccagac agtcgatcga atctgcagac cagagagtca catcaagaat
     4381 gttgacattc caccagaaga ctctaattat tcttcgctcg actgggaaaa ctgttaacaa
     4441 ctgctcgagt gtggtcggag cggctcacag tgcgttttcc cgtagacaaa aatcaaattt
     4501 catggtattt ttcggcgata aacagtaagt taaagggaac ccgaatttag tgctatataa
     4561 tttaattcca ctagagtttg tatcctttga tagatacgcg tatttcgacc tcaactgcaa
     4621 ggccgtcgtg tactagactt gactaatcca gactggtctt ttagttatga cttctgtcca
     4681 catctccata cattcaacgc actgtgcggc tgtgctgtgc gactccgtcg agtcgaccaa
     4741 catagttgaa acaaattgaa tatttaattg atcgttatag gaatggtgtt agatgagtca
     4801 tcctttacag taagcacata cagtattata attgaagatc gtcggcagat aggtgtgtag
     4861 ggtagagtat cagcaataag ttgggacgtt tgactttttg taggtagaca aaaactaaac
     4921 tttttttcgc ttctctatgt gtgcccctgg gtagcgttcc gttccgattg gggtgcgaac
     4981 gaatgaaatc gcccatcgag ttgatacgtc catccatcgc tagaaccgcg ttcgctgtag
     5041 aagactatat aagagcagag gcaagagtag tgaaatagcg acatgcagcc attctggtct
     5101 tagagctatg ctggaaacag catagcaagt taagataagg ctagtccgtt atcaacttga
     5161 aaaagtggca ccgagtcggt gctttttttg gtggttgttc atcgattcct aatattgcag
     5221 tgaagcagtt tgttgtgcat ggcgaagata aggttagggc gaggaatgcc ttggaaataa
     5281 gcggcactgg gccgtatgaa taaaaagcgt tgaactttac tacatgtagc atctactcaa
     5341 aaacaaaatc cacaaagttt actacacttt tggtatgaat aaaaaccttt tcgaacatgt
     5401 agtacctact actttaagaa aattctattc aaagtacaga gtaatgctgc atgttaagaa
     5461 aattccattc agagtgactc ttgaaatcaa taaaatcatg catatatgcc aaatttgtca
     5521 cttattcacg tgacaaatta tgtgaaaata tgaatattac tacattcccg ctatgcatcg
     5581 cgtaatcgat catgaaattt atgttggtta tgtaacttta cagtattccc tttatgtgca
     5641 tgtgaatttt ataatgattt gacaacaagg attttcctta gaattttcga attttagcta
     5701 gaaatctgta tcctatgaat gaatcttgag ataacagcaa ttgtaacatt ataaagttcc
     5761 taacattttc aagattccag tattgagtcg cgatcgctta attaaccgcg gtcatcacaa
     5821 acgaaactat tataatcgtc ttcaggggaa cggtagcatc atatctgtca ccagtcagtc
     5881 ggattccttt ctttcggcat aaattatgct cgggtgacga gacgagttga aaatcccgta
     5941 aattaaaaca tataccacac tcgccgtcct agacgacgac gacgacgacg attcccaagg
     6001 aattgaaccc ttcatttgca tttcactacc gctctgagag atgcaaagcg cgccagacag
     6061 tcgatcgaat ctgcagacca gagagtcaca tcaagaatgt tgacattcca ccagaagact
     6121 ctaattattc ttcgctcgac tgggaaaact gttaacaact gctcgagtgt ggtcggagcg
     6181 gctcacagtg cgttttcccg tagacaaaaa tcaaatttca tggtattttt cggcgataaa
     6241 cagtaagtta aagggaaccc gaatttagtg ctatataatt taattccact agagtttgta
     6301 tcctttgata gatacgcgta tttcgacctc aactgcaagg ccgtcgtgta ctagacttga
     6361 ctaatccaga ctggtctttt agttatgact tctgtccaca tctccataca ttcaacgcac
     6421 tgtgcggctg tgctgtgcga ctccgtcgag tcgaccaaca tagttgaaac aaattgaata
     6481 tttaattgat cgttatagga atggtgttag atgagtcatc ctttacagta agcacataca
     6541 gtattataat tgaagatcgt cggcagatag gtgtgtaggg tagagtatca gcaataagtt
     6601 gggacgtttg actttttgta ggtagacaaa aactaaactt tttttcgctt ctctatgtgt
     6661 gcccctgggt agcgttccgt tccgattggg gtgcgaacga atgaaatcgc ccatcgagtt
     6721 gatacgtcca tccatcgcta gaaccgcgtt cgctgtagaa gactatataa gagcagaggc
     6781 aagagtagtg aaatgcggga ttcgctctcg acgagtctta gagctatgct ggaaacagca
     6841 tagcaagtta agataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     6901 tttttttggt ggttgttcat cgattcctaa tattgcagtg aagcagtttg ttgtgcatgg
     6961 cgaagataag gttagggcga ggaatgcctt ggaaataagc ggcactgggc cgtatgaata
     7021 aaaagcgttg aactttacta catgtagcat ctactcaaaa acaaaatcca caaagtttac
     7081 tacacttttg gtatgaataa aaaccttttc gaacatgtag tacctactac tttaagaaaa
     7141 ttctattcaa agtacagagt aatgctgcat gttaagaaaa ttccattcag agtgactctt
     7201 gaaatcaata aaatcatgca tatatgccaa atttgtcact tattcacgtg acaaattatg
     7261 tgaaaatatg aatattacta cattcccgct atgcatcgcg taatcgatca tgaaatttat
     7321 gttggttatg taactttaca gtattccctt tatgtgcatg tgaattttat aatgatttga
     7381 caacaaggat tttccttaga attttcgaat tttagctaga aatctgtatc ctatgaatga
     7441 atcttgagat aacagcaatt gtaacattat aaagttccta acattttcaa gattccagta
     7501 ttgaggcctt ttgggcccat ttaaatctta agtcatcaca aacgaaacta ttataatcgt
     7561 cttcagggga acggtagcat catatctgtc accagtcagt cggattcctt tctttcggca
     7621 taaattatgc tcgggtgacg agacgagttg aaaatcccgt aaattaaaac atataccaca
     7681 ctcgccgtcc tagacgacga cgacgacgac gattcccaag gaattgaacc cttcatttgc
     7741 atttcactac cgctctgaga gatgcaaagc gcgccagaca gtcgatcgaa tctgcagacc
     7801 agagagtcac atcaagaatg ttgacattcc accagaagac tctaattatt cttcgctcga
     7861 ctgggaaaac tgttaacaac tgctcgagtg tggtcggagc ggctcacagt gcgttttccc
     7921 gtagacaaaa atcaaatttc atggtatttt tcggcgataa acagtaagtt aaagggaacc
     7981 cgaatttagt gctatataat ttaattccac tagagtttgt atcctttgat agatacgcgt
     8041 atttcgacct caactgcaag gccgtcgtgt actagacttg actaatccag actggtcttt
     8101 tagttatgac ttctgtccac atctccatac attcaacgca ctgtgcggct gtgctgtgcg
     8161 actccgtcga gtcgaccaac atagttgaaa caaattgaat atttaattga tcgttatagg
     8221 aatggtgtta gatgagtcat cctttacagt aagcacatac agtattataa ttgaagatcg
     8281 tcggcagata ggtgtgtagg gtagagtatc agcaataagt tgggacgttt gactttttgt
     8341 aggtagacaa aaactaaact ttttttcgct tctctatgtg tgcccctggg tagcgttccg
     8401 ttccgattgg ggtgcgaacg aatgaaatcg cccatcgagt tgatacgtcc atccatcgct
     8461 agaaccgcgt tcgctgtaga agactatata agagcagagg caagagtagt gaaatgatag
     8521 gagccaagtt ctggggtctt agagctatgc tggaaacagc atagcaagtt aagataaggc
     8581 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttgg tggttgttca
     8641 tcgattccta atattgcagt gaagcagttt gttgtgcatg gcgaagataa ggttagggcg
     8701 aggaatgcct tggaaataag cggcactggg ccgtatgaat aaaaagcgtt gaactttact
     8761 acatgtagca tctactcaaa aacaaaatcc acaaagttta ctacactttt ggtatgaata
     8821 aaaacctttt cgaacatgta gtacctacta ctttaagaaa attctattca aagtacagag
     8881 taatgctgca tgttaagaaa attccattca gagtgactct tgaaatcaat aaaatcatgc
     8941 atatatgcca aatttgtcac ttattcacgt gacaaattat gtgaaaatat gaatattact
     9001 acattcccgc tatgcatcgc gtaatcgatc atgaaattta tgttggttat gtaactttac
     9061 agtattccct ttatgtgcat gtgaatttta taatgatttg acaacaagga ttttccttag
     9121 aattttcgaa ttttagctag aaatctgtat cctatgaatg aatcttgaga taacagcaat
     9181 tgtaacatta taaagttcct aacattttca agattccagt attgagtcgc gatcgcttaa
     9241 ttaaccgcgg atatgtcttg ccgaagcaca ttaacccaag ccatttatat atcaagtaca
     9301 agttgatttg attctagtga tggactgaca cgaatctatg gcactgttgc agagcttaca
     9361 aacaggtacc cggagtcgct tcaattattc aatcatccat tcaaaaactt ccatctcttc
     9421 caattagcca atattcaaat tggaatccag ccagccgacg gaggcagatg gtctacctgg
     9481 caactataga gtttccatgt ttccagactt tccctcccgg taaacggaga caaaacgaca
     9541 gacgtaagta ggtacatatg cataccgcac ggacaaatca aatttgtctg gcagctccaa
     9601 ttagagtcgt taaaaattta acgatgcgct aaataacttc aagctatttg tctcgctgga
     9661 ttggcttcga gtggtaagat cctatcaaat gccgaaaaca aaaaactttc ttcttaattg
     9721 ttcgttcttc aacacctctc catggtgata acggatacgg tttcattgtc agcatccatc
     9781 ctccgaaaaa tacattacgc cttgaaatat gcaatcgcaa acacggatct gtttggaaca
     9841 tttattttac tatgaagaga tgcgataggt aatatttatt tgagcgttta agatactcat
     9901 tgttctctca aagaatgtca ttgaaagcca acgaggtcaa atcaaatatt ataataaaaa
     9961 ggtcaaagag gactaactta aagctctctt tatggatagg aaaaaatatt ttcgcccatc
    10021 gctagaactt ttaccgtttc cattgagtat ataactaaga tgaatgaggc taattgatgt
    10081 ctccgcaata cgccccgggt cttagagcta tgctggaaac agcatagcaa gttaagataa
    10141 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt taagatgctt
    10201 cggcaattaa taataaggaa tggtaaagca ttcttcaaat gttactatgt tctcaaattt
    10261 tcaagaatta ggtattcaag aaattctttc aggcatattc cataatctgc acaagcagct
    10321 actttagaaa ttattgccgg gcttaaatta tatgtacaac atttcctgag tattttctga
    10381 caaaaattct cacaagggaa ggtcacgatg gagttacacg gggtttttaa ccggactatt
    10441 tttgttagat tctacatgtc gaaatatgaa aaaccccaaa gcctttcggg tgatggacca
    10501 gtggtgtagc caggaggggc tctgaaaggg gtaattttgt acgtatatta tattattaag
    10561 ctaattggaa atttgacaaa aatatttttt tagtatttat tgtgatggta gagaacaaaa
    10621 ttgacattaa tgtacgtttg aaatgtattt gccatgcctt tggcgtacta acatggagta
    10681 tggacaccaa aaccgatttc gctagcgttt aaacggcgcg ccaattcgag ctcgcccggg
    10741 gatctaattc aattagagac taattcaatt agagctaatt caattaggat ccaagcttat
    10801 cgatttcgaa ccctcgaccg ccggagtata aatagaggcg cttcgtctac ggagcgacaa
    10861 ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc
    10921 gcagctgaac aagctaaaca atcggggtac cgctagagtc gacggtacga tccaccggtc
    10981 gccaccatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc
    11041 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
    11101 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
    11161 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
    11221 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
    11281 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
    11341 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
    11401 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
    11461 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
    11521 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
    11581 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
    11641 gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc
    11701 ggcaccgcct cctccgagga caacaacatg gccgttatca aggaatttat gcgcttcaaa
    11761 gttaggatgg agggatccat gaacggacat gagttcgaga tcgagggaga gggcgaggga
    11821 cgcccgtatg aaggcacaca aacagccaaa ctcaaggtca ccaagggcgg accactgccc
    11881 ttcgcctggg atatcctgag tccccagttt atgtacggca gcaaggccta cgttaagcac
    11941 cccgctgaca taccggacta caaaaagctg tcctttccgg aaggcttcaa gtgggagcgc
    12001 gtgatgaatt tcgaagacgg aggactggtc actgtgaccc aagatagcag tttgcaggac
    12061 ggtacactga tctataaggt taaaatgcgc ggcactaact ttccgccaga tggcccagtg
    12121 atgcagaaga agaccatggg ttgggaggca tccaccgaac gtctgtaccc tcgagacgga
    12181 gtgctcaagg gcgagatcca tcaggccctc aaactgaaag atggtggtca ctacctggtc
    12241 gaatttaaga ccatttacat ggccaagaag ccggttcagc tgcccggata ttattatgtg
    12301 gatacgaaac tggatataac ttcgcataac gaagactaca ccattgtcga gcagtatgag
    12361 cgcagcgaag gccgacatca cctgttcctc tacggcatgg acgagctgta caagtaggcg
    12421 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
    12481 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
    12541 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    12601 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    12661 aagcttgcta gctcatcaca aacgaaacta ttataatcgt cttcagggga acggtagcat
    12721 catatctgtc accagtcagt cggattcctt tctttcggca taaattatgc tcgggtgacg
    12781 agacgagttg aaaatcccgt aaattaaaac atataccaca ctcgccgtcc tagacgacga
    12841 cgacgacgac gattcccaag gaattgaacc cttcatttgc atttcactac cgctctgaga
    12901 gatgcaaagc gcgccagaca gtcgatcgaa tctgcagacc agagagtcac atcaagaatg
    12961 ttgacattcc accagaagac tctaattatt cttcgctcga ctgggaaaac tgttaacaac
    13021 tgctcgagtg tggtcggagc ggctcacagt gcgttttccc gtagacaaaa atcaaatttc
    13081 atggtatttt tcggcgataa acagtaagtt aaagggaacc cgaatttagt gctatataat
    13141 ttaattccac tagagtttgt atcctttgat agatacgcgt atttcgacct caactgcaag
    13201 gccgtcgtgt actagacttg actaatccag actggtcttt tagttatgac ttctgtccac
    13261 atctccatac attcaacgca ctgtgcggct gtgctgtgcg actccgtcga gtcgaccaac
    13321 atagttgaaa caaattgaat atttaattga tcgttatagg aatggtgtta gatgagtcat
    13381 cctttacagt aagcacatac agtattataa ttgaagatcg tcggcagata ggtgtgtagg
    13441 gtagagtatc agcaataagt tgggacgttt gactttttgt aggtagacaa aaactaaact
    13501 ttttttcgct tctctatgtg tgcccctggg tagcgttccg ttccgattgg ggtgcgaacg
    13561 aatgaaatcg cccatcgagt tgatacgtcc atccatcgct agaaccgcgt tcgctgtaga
    13621 agactatata agagcagagg caagagtagt gaaatctcac aagtagagct acacggtctt
    13681 agagctatgc tggaaacagc atagcaagtt aagataaggc tagtccgtta tcaacttgaa
    13741 aaagtggcac cgagtcggtg ctttttttgg tggttgttca tcgattccta atattgcagt
    13801 gaagcagttt gttgtgcatg gcgaagataa ggttagggcg aggaatgcct tggaaataag
    13861 cggcactggg ccgtatgaat aaaaagcgtt gaactttact acatgtagca tctactcaaa
    13921 aacaaaatcc acaaagttta ctacactttt ggtatgaata aaaacctttt cgaacatgta
    13981 gtacctacta ctttaagaaa attctattca aagtacagag taatgctgca tgttaagaaa
    14041 attccattca gagtgactct tgaaatcaat aaaatcatgc atatatgcca aatttgtcac
    14101 ttattcacgt gacaaattat gtgaaaatat gaatattact acattcccgc tatgcatcgc
    14161 gtaatcgatc atgaaattta tgttggttat gtaactttac agtattccct ttatgtgcat
    14221 gtgaatttta taatgatttg acaacaagga ttttccttag aattttcgaa ttttagctag
    14281 aaatctgtat cctatgaatg aatcttgaga taacagcaat tgtaacatta taaagttcct
    14341 aacattttca agattccagt attgagtcgc gatcgcttaa ttaaccgcgg atatgtcttg
    14401 ccgaagcaca ttaacccaag ccatttatat atcaagtaca agttgatttg attctagtga
    14461 tggactgaca cgaatctatg gcactgttgc agagcttaca aacaggtacc cggagtcgct
    14521 tcaattattc aatcatccat tcaaaaactt ccatctcttc caattagcca atattcaaat
    14581 tggaatccag ccagccgacg gaggcagatg gtctacctgg caactataga gtttccatgt
    14641 ttccagactt tccctcccgg taaacggaga caaaacgaca gacgtaagta ggtacatatg
    14701 cataccgcac ggacaaatca aatttgtctg gcagctccaa ttagagtcgt taaaaattta
    14761 acgatgcgct aaataacttc aagctatttg tctcgctgga ttggcttcga gtggtaagat
    14821 cctatcaaat gccgaaaaca aaaaactttc ttcttaattg ttcgttcttc aacacctctc
    14881 catggtgata acggatacgg tttcattgtc agcatccatc ctccgaaaaa tacattacgc
    14941 cttgaaatat gcaatcgcaa acacggatct gtttggaaca tttattttac tatgaagaga
    15001 tgcgataggt aatatttatt tgagcgttta agatactcat tgttctctca aagaatgtca
    15061 ttgaaagcca acgaggtcaa atcaaatatt ataataaaaa ggtcaaagag gactaactta
    15121 aagctctctt tatggatagg aaaaaatatt ttcgcccatc gctagaactt ttaccgtttc
    15181 cattgagtat ataactaaga tgaatgaggc taattgatct gtcgtcgttt ttttccgggt
    15241 cttagagcta tgctggaaac agcatagcaa gttaagataa ggctagtccg ttatcaactt
    15301 gaaaaagtgg caccgagtcg gtgctttttt taagatgctt cggcaattaa taataaggaa
    15361 tggtaaagca ttcttcaaat gttactatgt tctcaaattt tcaagaatta ggtattcaag
    15421 aaattctttc aggcatattc cataatctgc acaagcagct actttagaaa ttattgccgg
    15481 gcttaaatta tatgtacaac atttcctgag tattttctga caaaaattct cacaagggaa
    15541 ggtcacgatg gagttacacg gggtttttaa ccggactatt tttgttagat tctacatgtc
    15601 gaaatatgaa aaaccccaaa gcctttcggg tgatggacca gtggtgtagc caggaggggc
    15661 tctgaaaggg gtaattttgt acgtatatta tattattaag ctaattggaa atttgacaaa
    15721 aatatttttt tagtatttat tgtgatggta gagaacaaaa ttgacattaa tgtacgtttg
    15781 aaatgtattt gccatgcctt tggcgtacta acatggagta tggacaccaa aaccgatttc
    15841 gtttaaacga tctcggatct gacaatgttc agtgcagaga ctcggctacg cctcgtggac
    15901 tttgaagttg accaacaatg tttattctta cctctaatag tcctctgtgg caaggtcaag
    15961 attctgttag aagccaatga agaacctggt tgttcaataa cattttgttc gtctaatatt
    16021 tcactaccgc ttgacgttgg ctgcacttca tgtacctcat ctataaacgc ttcttctgta
    16081 tcgctctgga cgtcatcttc acttacgtga tctgatattt cactgtcaga atcctcacca
    16141 acaagctcgt catcgctttg cagaagagca gagaggatat gctcatcgtc taaagaacta
    16201 cccattttat tatatattag tcacgatatc tataacaaga aaatatatat ataataagtt
    16261 atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt
    16321 cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt
    16381 gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc
    16441 taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca
    16501 tgcgtcaatt ttacgcagac tatctttcta gggttaaaaa agatttgcgc tttactcgac
    16561 ctaaacttta aacacgtcat agaatcttcg tttgacaaaa accacattgt ggccaagctg
    16621 tgtgacgcga cgcgcgctaa agaatggcaa accaagtcgc gcgagcgtcg actctagagg
    16681 atccccgggt accgagctcg aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
    16741 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    16801 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    16861 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    16921 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    16981 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg t
//
