LOCUS       sequence-393035-        7423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7423)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 7423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-Palladin-C-7
FEATURES             Location/Qualifiers
     source          1..7423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..1980
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    4151..4272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4188..4207)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4242..4261
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4279..4734)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4416..4437)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4628..4647
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4761..4865
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4867..5224
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4867..5058
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4890..4910)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5075..5210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5259..6053
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5259..6053
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5313..5332)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5923..5942
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6241..6260)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6285..6332
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6661..7249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7150..7169
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag
      601 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
      661 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
      721 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
      781 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
      841 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
      901 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
      961 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
     1021 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
     1081 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
     1141 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     1201 cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc
     1261 agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc
     1321 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     1381 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     1441 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
     1501 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
     1561 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
     1621 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
     1681 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
     1741 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
     1801 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
     1861 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     1921 tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag
     1981 tccggactca gatctcgagt gagcgctctg gcctcccgcg ctaccgccat gcagtcctcc
     2041 ggctccttca actacgcgcg ccctaagcag ttcatcgcgg cgcagaacct gggtcccgca
     2101 tcggggctgc ccacgccaac ctccagcccc agctcctcca gcttgccgtc accattgtcc
     2161 cccacgccca ggcccttcgg ccgcgcgccc gggccgccct tcgtggaacc cgaggccatg
     2221 tggggcccgt cctcgccctc gccgccaccg ccgccacctc cggtcttcag cccctctgca
     2281 gcctacccgg tgcccgacgt gttcccactg ccaccgccgc cgccaccgct acctagctcc
     2341 acctcacact gcgcctcgcc cgcccgcttc ggccccagcc agacgcccgc agccttcctc
     2401 agcgccctgc tgccctctca gccaccgcct gtcgctgtca acgccctggg gctgcccaag
     2461 ggagtcaccc ccgcggggtt cccaaagaag tccagtagaa ctgctagaat tgcctctgat
     2521 gaggagattc aaggcacaaa ggatgctgtc atccaagacc tggaacggaa gcttcgcttc
     2581 aaggaggacc ttctgaacaa tggccaaccg aggctaacct atgaggaaag aatggctcgc
     2641 cgcctgcttg gagccgacag cgcaaacgtc ttcaacatcc aggagccaga ggaaacggca
     2701 gccaatcagg agtacaaagt ctctagctgc gagcagaggc tgattagcga gattgaatac
     2761 aggctggagc gctcccctgt ggatgagtcg ggagacgagg tgcaggatcc agatgtgcct
     2821 gtggagaacg caacagctcc cttctttgag atgaagctga aacactacaa gatctttgag
     2881 gggatgccgg tgactttcac gtgtcgagtg gctgggaatc caaagccaaa gatctattgg
     2941 tttaaagatg ggaagcagat ttctccgaag agcgatcact acaccattca gagagacctt
     3001 gatgggacct gctctctcca caccacggcc tctaccctag acgacgatgg gaactacacc
     3061 atcatggctg ccaaccctca gggtcgcgtc agttgtacag gaaggctaat ggtacaggct
     3121 gtcaaccaaa gaggccgcag tccccgctct ccctcaggcc atcctcatgc cagaaggcct
     3181 cgctctcgat cacgggacag tggagatgaa aacgagccca ttcaggagcg attcttcaga
     3241 cctcacttcc tgcaggctcc tggagacctg accgttcagg aaggcaagct ctgcaggatg
     3301 gactgcaagg tcagtgggtt accaacccca gatctcagct ggcaactaga cggaaagccc
     3361 atacgccccg acagtgctca caagatgctg gtccgtgaga atggggtcca ctccctcatt
     3421 atagagccag tcacgtcccg ggacgccggc atctacacat gtattgccac caacagagca
     3481 ggacagaact cgtttaacct ggagcttgtg gttgctgcta aggaagcaca caaggcccct
     3541 gtgtttatgg agaagctaca gaacacgggg gttgctgatg gatacccagt gcggctagaa
     3601 tgccgtgtct ccggagtgcc gccacctcag atattttgga agaaagaaaa tgaatcgctc
     3661 actcatagca ctgagcgagt aagcatgcac caggataatc atggctacat ctgcctgctc
     3721 atccagggag ccacaaagga agacgctggg tggtacactg tgtctgccaa gaacgaagca
     3781 ggcattgtgt cctgcactgc ccggctggat gtctacaccc agtggcacca gcagccacag
     3841 accaccaagc caaaaaaagt acggccctcg gccagtcgct acgcagcact ttcggaccag
     3901 ggactagaca tcaaagccgc tttccaacct gaagccagcc catctcacct gactctgaac
     3961 agcggcttgg tagaaagtga agacctgtga gaattctgca gtcgacggta ccgcgggccc
     4021 gggatccacc ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt
     4081 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     4141 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4201 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4261 caatgtatct taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     4321 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     4381 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     4441 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     4501 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     4561 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     4621 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     4681 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     4741 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4801 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4861 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     4921 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4981 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5041 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5101 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5161 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5221 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     5281 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     5341 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     5401 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     5461 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     5521 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     5581 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     5641 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     5701 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     5761 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     5821 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     5881 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     5941 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     6001 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     6061 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     6121 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     6181 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     6241 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     6301 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     6361 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     6421 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     6481 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     6541 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6601 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6661 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6721 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6781 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6841 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6901 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6961 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7021 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7081 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7141 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7201 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7261 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7321 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
     7381 caattacggg gtcattagtt catagcccat atatggagtt ccg
//
