LOCUS       sequence-393039-        3966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC16A11_STOP
FEATURES             Location/Qualifiers
     source          1..3966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      961..970
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(1523..1622)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1639..1658)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1640..1658)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1663..1679)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1663..1679)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1781..1800)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1792..2601
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1859..1878)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2748..3336
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3237..3256
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3490..3507
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3666..3693)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3785..3871)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3920..3942
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3934..3951
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3935..3951
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgcc tgccccacag
      121 agaaagcacc ggagaggcgg cttctcccac aggtgctttc ccacccctca gacagccatg
      181 accccacagc cagcaggacc acctgacgga ggatggggct gggtggtggc cgccgccgcc
      241 ttcgccatca acggcctgtc ctacggactg ctgcgctctc tgggactggc attcccagat
      301 ctggcagagc actttgaccg gtctgcccag gatacagcct ggatcagcgc cctggccctg
      361 gccgtgcagc aggccgccag ccccgtgggc agcgccctgt ccaccagatg gggagcaagg
      421 cctgtggtca tggtgggagg cgtgctggca tccctgggct tcgtgttttc cgccttcgcc
      481 tctgacctgc tgcacctgta cctgggactg ggactgctgg caggattcgg atgggcactg
      541 gtgtttgcac ctgccctggg cacactgagc cgctatttct ccaggcgccg ggtgctggca
      601 gtgggactgg ccctgaccgg caatggagca agctccctgc tgctggcacc cgccctgcag
      661 ctgctgctgg acacatttgg ctggagaggc gccctgctgc tgctgggagc catcacactg
      721 cacctgaccc catgcggcgc cctgctgctg cctctggtgc tgccaggcga tccaccagca
      781 cctccaaggt ccccactggc cgccctgggc ctgtctctgt tcacaagaag ggccttcagc
      841 atctttgcac tgggaaccgc cctggtggga ggaggatact ttgtgcccta tgtgcacctg
      901 gcccctcacg ccctggatag gggactggga ggatacggag ccgccctggt ggtggccgtg
      961 gccgccatgg gcgacgccgg cgccagactg gtgtgcggat ggctggcaga tcagggatgg
     1021 gtgcctctgc caaggctgct ggccgtgttc ggcgccctga caggactggg actgtgggtg
     1081 gtgggactgg tgcctgtggt gggaggcgag gagtcttggg gaggaccact gctggcagca
     1141 gcagtggcct acggcctgtc tgccggcagc tatgcaccac tggtgtttgg cgtgctgcca
     1201 ggactggtgg gagtgggagg cgtggtgcag gccaccggcc tggtcatgat gctgatgtcc
     1261 ctgggaggac tgctgggacc acctctgtct ggcttcctgc gcgacgagac aggcgatttc
     1321 accgccagct ttctgctgtc tggcagcctg atcctgagcg gctcttttat ctatatcgga
     1381 ctgccacggg ccctgccttc ttgcggccca gccagcccac ccgccacacc tccacccgag
     1441 accggagagc tgctgccagc acctcaggcc gtgctgctga gcccaggcgg ccccggctcc
     1501 accctggaca ccacatgtta agacccagct ttcttgtaca aagttggcat tataagaaag
     1561 cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
     1621 tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
     1681 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     1741 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     1801 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     1861 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     1921 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     1981 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     2041 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     2101 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     2161 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     2221 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     2281 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     2341 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     2401 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2461 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2521 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2581 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2641 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     2701 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2761 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2821 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2881 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2941 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     3001 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     3061 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     3121 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3181 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3241 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3301 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3361 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3421 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3481 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     3541 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     3601 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
     3661 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
     3721 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
     3781 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     3841 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
     3901 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
     3961 gggccc
//
