LOCUS       sequence-393051-       10999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10999)
  TITLE     Sean Wu lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pNK-TGCK
FEATURES             Location/Qualifiers
     source          1..10999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          2725..2954
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     2937..2956
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3003..3623
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     polyA_signal    3773..3907
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4269..4891
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4468..4487)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     protein_bind    4917..4935
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4959..4977
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5001..5019
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5043..5061
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5085..5103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     5130..5154
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5272..5281
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5278..5280,5281..5283,5284..5994)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5278..5280,5281..5283,5284..5994)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5584..5603)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5931..5952
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6049..7080
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     polyA_signal    7787..7908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(7915..8370)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8052..8073)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8264..8283
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8397..8501
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8503..8860
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8503..8694
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8526..8546)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8711..8846
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8895..9689
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8895..9689
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8949..8968)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9559..9578
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9877..9896)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9921..9968
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10297..10885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10786..10805
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattac tagcgctacc ggactcagat ctcgagctcc accgcggtgg cggccgctct
       61 agaactagtc cagtctgggt cctaatgcgg gtggcgtctc tcttgacagg cagcgtttgg
      121 ggacaacagc ggggaaggga gataagatga cataccagag cagatttggt gtgcgcgctg
      181 atactcctgg cccgacagga aactcggagc tatttaaaaa ggccctatcg attactttat
      241 cttccccgga ggaaaacttc ttgccgagag acaaaagatg tccccctacc taaagataca
      301 aggccacaca gcagagggtt tgtacaggcg acgcgcgagt agatccctgg gagcgcagag
      361 acgcctttct accggcagag actgaagttt gactggagcg aggcggggcg gagccacctc
      421 ccggtccccg tgcactccgg aattgtgaac gctcccgcaa agtccccgcg agtgttgtgt
      481 tcacactaat tggtgtgcct ctgagttcca tccgtactcc ccccccccca agtttaaatg
      541 ctccttttaa gggcttgagt gtctgcagcc gtcatgtgca ccttgaaagc ttggcgtgtg
      601 acattgtgtt cctctagcgt ctccatcccg cttttcacca attcgtgccc tggccgaatc
      661 ccgtgggagc agaggtggcc cctcgaagtc ccgggccacc cagggaggct ccgggtacac
      721 ccagattccc agcagtccgt agtctgggct ttccccgtgt gacactttgc cgcctaccgg
      781 ttcacaatcc tctcggcccg cgtgagagaa ggcggcatgt tccgtgggac acagtttatt
      841 ttatgcacat taagggctgt aaagcacggt ctgaaagaaa aagatggtgc ggcggggagg
      901 agcgggagaa ggggcagcga tgatcctggg actgtgcagc gaccacttac aacctgattc
      961 atgcccaccg gggaccccag cggtcctgga gggaggcgga ggctccgagg cttccggttg
     1021 ggtttcagcc accaggttcg ggcagtgact cttggactct gaccacgtcc cgcggcacac
     1081 tcggagaccc ggctcgtccg cgaagctagg agcgaatggc ccgcagctgc gcgtcgctca
     1141 atctgagctc tcaagcccaa agcgtctgca gagccgcttc ccagagctcc aagccaccgc
     1201 tagcagagct ctgaaggcac cttgaagcct cgacccactt caatgccacc tgatttttca
     1261 aattatcacc ttctgagacg cgatccttcg gaaccggcag agcccccacc tttgagcgcc
     1321 gccgtttatg ctgtagcgct gtagcggttt gcagcagtca gggcaggtgg aggaccttgt
     1381 tactggcact gcggagcccg gagctgaggg gaaagaaaac ggagataagg aaaagggctg
     1441 cagaggtcgg aaagcagctc ccatcggatc catcaaatca atcacagccc caagtgacca
     1501 aagagaaatt ttgcgttttg gaaatatccc caccaccaaa agcacgagga aagtgtgagc
     1561 agcaagaaac taacaataaa ttttaggtgc aaaaatataa agaagcacac agaatttgaa
     1621 aaaaaaaacc cgcacattct gctatttgag ttttccataa acatacaaat aaaacctgcg
     1681 gaagatgtac ttcttcaagt ctgtgacaga ccctggactg actaacgggg acgcgctcat
     1741 ttaaaatgtc agcggttcct atatttcacc gaactttcaa cgagcttttc cacatgtcca
     1801 gactcatggg acagtatgct tatgaccctg ttaattgtag ttcagactta ctatgtctca
     1861 ataaagtgac tcagcacttg accagcatat gaggctcccc gagtccagtc cccagaaaca
     1921 agtagaatat acatatatat ataattctat atatctatct atatattatt ctatatatat
     1981 attctacttg tatatatatg tattacataa tatatataca agtagaatat atataatata
     2041 atttatatta tatatagaat atatatagaa wmwagtatat atatatatat atatatatat
     2101 atatagtatt gtattacata catgtgggta tacatcatac acaggactag tggatccccc
     2161 agcttggggc tttcaatcca tcctcaccag cccatttagt gatacaggac acagtgcaaa
     2221 gaatatttat tcaaatcctc gacccacccc caaccctgcg tttagactca gcataacaga
     2281 atcaggtatt ttctttgagt gtgtctggct gggattttca ggctaacgag gagcaatctc
     2341 cttgcaggag tgggagacta tcccaaatgt gtcgcggccc ctttaaaaaa agctgagcag
     2401 gatttttttt tcccatccct atgtctatat taggtgacgc agaactgccc gtcgctcctg
     2461 tcatccaggc ccctggccca atggcaggct gaatcccccc tactccagcc tgcycccgcc
     2521 tctctgccct ggmtgwrkcs cscskmsksc rgcskgcwcc mksckctctg ccctggatgt
     2581 ggcgcgcggc gggcggcggg caccatgcgg ggaggctgtc cccaggggtg ggcagcacca
     2641 ctctctgcta cccacctggc ccaagctggg tacctgccaa gctctagttc tggaattcgc
     2701 tgtctgcgag ggccagctgt tggggtgagt actccctctc aaaagcgggc atgacttctg
     2761 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgcggtga
     2821 tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg ttgtcaagct
     2881 tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc
     2941 ctttctctcc acaggtgtcc actcccaggt ccaactgcag cccaagcgga tccgaattca
     3001 ccatgtccag actggacaag agcaaagtca tcaactctgc cttggagctc ctgaatgaag
     3061 ttggcattga gggcttgacc accaggaagc tggcccagaa gctgggtgtg gagcagccta
     3121 ccctgtactg gcatgtgaag aacaagaggg ctctgcttga tgccctggcc attgagatgt
     3181 tggacaggca ccacacccac ttctgccctc tggaagggga gtcctggcag gacttcctga
     3241 ggaacaatgc caagagcttc agatgtgcct tgctctccca ccgggatggt gccaaagttc
     3301 acttgggcac caggcctaca gagaagcagt atgagaccct ggagaaccag ctggcattcc
     3361 tgtgccaaca aggcttctcc ctggaaaatg ccttgtatgc cctctctgct gtgggccact
     3421 tcaccttggg ctgtgtgctg gaggaccagg agcaccaagt tgccaaggag gagagggaga
     3481 cccccaccac tgactccatg ccaccactgc tgcggcaagc tattgagttg tttgaccacc
     3541 aaggggctga gcctgcattc ctttttggcc tggaactgat catctgtggc ctggaaaagc
     3601 agctgaaatg tgagtctggc tctcacgtgc ccaaaaagag aaagcacgtg cctgctgatg
     3661 ccttggatga ttttgacctg gacatgctgc ctgctgatgc cctggatgac tttgatttgg
     3721 acatgctccc tgctgatgca cttgatgatt ttgacctgga tatgctgtga tggatccaga
     3781 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     3841 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     3901 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     3961 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctgcaag
     4021 cctcgtcgtc ctggccggac cacgctatct gtgcaaggtc cccggccccg gacgcgcgct
     4081 ccatgagcag agcgcccgcc gccgaggcga agactcgggc ggcgccctgc ccgtcccacc
     4141 aggtcaacag gcggtaacgt aaccggcctc ttcatcggga atgcgcgcga acttcagcat
     4201 cgccggcatg tccccctggc ggacgggaag tatccagctc gaccaagctt ctcgacgata
     4261 gatctcccgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg
     4321 ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact
     4381 aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg
     4441 ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac
     4501 aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc
     4561 ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt
     4621 agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct
     4681 acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg
     4741 tccttctgat tttaaaataa ctataccagc aggaggacgt ccagacacag cataggctac
     4801 ctggccatgc ccaaccggtg ggacatttga gttgcttgct tggcactgtc ctctcatgcg
     4861 ttgggtccac tcagtagatg cctgttgaat tcggtaccga gctcgacttt cacttttctc
     4921 tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg
     4981 gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc gactttcact
     5041 tttctctatc actgataggg agtggtaaac tcgactttca cttttctcta tcactgatag
     5101 ggagtggtaa actcgaccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     5161 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc
     5221 gtccccgaat tcctgcactt aaagcttctc gagtctagat ctgcaccggt cgccaccatg
     5281 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     5341 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     5401 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     5461 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     5521 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     5581 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     5641 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     5701 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     5761 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     5821 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     5881 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     5941 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccggt
     6001 acagctctcg acggagaaag ctcaggctct ggctcagagt ctgactccat ggccaattta
     6061 ctgaccgtac accaaaattt gcctgcatta ccggtcgatg caacgagtga tgaggttcgc
     6121 aagaacctga tggacatgtt cagggatcgc caggcgtttt ctgagcatac ctggaaaatg
     6181 cttctgtccg tttgccggtc gtgggcggca tggtgcaagt tgaataaccg gaaatggttt
     6241 cccgcagaac ctgaagatgt tcgcgattat cttctatatc ttcaggcgcg cggtctggca
     6301 gtaaaaacta tccagcaaca tttgggccag ctaaacatgc ttcatcgtcg gtccgggctg
     6361 ccacgaccaa gtgacagcaa tgctgtttca ctggttatgc ggcggatccg aaaagaaaac
     6421 gttgatgccg gtgaacgtgc aaaacaggct ctagcgttcg aacgcactga tttcgaccag
     6481 gttcgttcac tcatggaaaa tagcgatcgc tgccaggata tacgtaatct ggcatttctg
     6541 gggattgctt ataacaccct gttacgtata gccgaaattg ccaggatcag ggttaaagat
     6601 atctcacgta ctgacggtgg gagaatgtta atccatattg gcagaacgaa aacgctggtt
     6661 agcaccgcag gtgtagagaa ggcacttagc ctgggggtaa ctaaactggt cgagcgatgg
     6721 atttccgtct ctggtgtagc tgatgatccg aataactacc tgttttgccg ggtcagaaaa
     6781 aatggtgttg ccgcgccatc tgccaccagc cagctatcaa ctcgcgccct ggaagggatt
     6841 tttgaagcaa ctcatcgatt gatttacggc gctaaggatg actctggtca gagatacctg
     6901 gcctggtctg gacacagtgc ccgtgtcgga gccgcgcgag atatggcccg cgctggagtt
     6961 tcaataccgg agatcatgca agctggtggc tggaccaatg taaatattgt catgaactat
     7021 atccgtaacc tggatagtga aacaggggca atggtgcgcc tgctggaaga tggcgattag
     7081 ccattaacgc gtaaatgatt gctctagagt gagggtcccc acctgggacc cttgagagta
     7141 tcaggtctcc cacgtgggag acaagaaatc cctgtttaat atttaaacag cagtgttccc
     7201 catctgggtc cttgcacccc tcactctggc ctcagccgac tgcwcagcrg mscykgsaks
     7261 cmykkssctg kgngtcccac gaatttgctg gggaatctcg tttttcttct taagactttt
     7321 gggacatggt ttgactcccg aacatcaccg acgtgtctcc tgtttttctg ggtggcctcg
     7381 ggacacctgc cctgccccca cgagggtcag gactgtgact ctttttaggg ccaggcaggt
     7441 gcctggacat ttgccttgct ggacggggac tggggatgtg ggagggagca gacaggagga
     7501 atcatgtcag gcctgtgtgt gaaaggaagc tccactgtca ccctccacct cttcaccccc
     7561 cactcaccag tgtcccctcc actgtcacat tgtaactgaa cttcaggata ataaagtgtt
     7621 tgcctccaaa aaaaaaaaaa aaaaaccgaa ttcagcttga agttcgcggc cgcgactcta
     7681 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     7741 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     7801 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7861 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
     7921 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     7981 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     8041 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     8101 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     8161 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     8221 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     8281 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     8341 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     8401 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8461 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
     8521 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     8581 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     8641 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     8701 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8761 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8821 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
     8881 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     8941 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     9001 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     9061 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     9121 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     9181 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     9241 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     9301 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     9361 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     9421 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     9481 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     9541 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     9601 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     9661 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     9721 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     9781 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     9841 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
     9901 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     9961 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
    10021 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
    10081 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
    10141 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    10201 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    10261 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    10321 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10381 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10441 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10501 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10561 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    10621 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    10681 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    10741 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    10801 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    10861 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    10921 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    10981 ccgtattacc gccatgcat
//
