LOCUS       sequence-393052-        7267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast two-hybrid ; Gal4 DNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Uetz P, Giot L, Cagney G, Mansfield TA, Judson RS, Knight JR, 
            Lockshon D, Narayan V, Srinivasan M, Pochart P, Qureshi-Emili A, Li 
            Y, Godwin B, Conover D, Kalbfleisch T, Vijayadamodar G, Yang M, 
            Johnston M, Fields S, Rothberg JM
  TITLE     A comprehensive analysis of protein-protein interactions in 
            Saccharomyces cerevisiae.
  JOURNAL   Nature. 2000 Feb 10;403(6770):623-7. doi: 10.1038/35001009.
  PUBMED    10688190
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2000-02-10- 10"; volume: "403"; issue: "6770"; 
            pages: "623-7"
COMMENT     Sequence Label: pOBD2
FEATURES             Location/Qualifiers
     source          1..7267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             536..976
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     terminator      1458..1645
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1513..1537)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     misc_feature    2475..3637
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      2800..3637
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     CDS             complement(3476..4150)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(4151..4252)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(4272..4290)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4358..4462
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4463..4531,4532..5323)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4681..4700)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5494..6082
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5983..6002
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6236..6253
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(7071..7267,1..508)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 gtggtacata acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt
       61 ttcactaccc tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt
      121 ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa
      181 aaatgatgga agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt
      241 tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattca
      301 cgcacactac tctctaatga gcaacggtat acggccttcc ttccagttac ttgaatttga
      361 aataaaaaaa agtttgctgt cttgctatca agtataaata gacctgcaat tattaatctt
      421 ttgtttcctc gtcattgttc tcgttccctt tcttccttgt ttctttttct gcacaatatt
      481 tcaagctata ccaagcatac aatcaactcc aagcttgaag caagcctcct gaaagatgaa
      541 gctactgtct tctatcgaac aagcatgcga tatttgccga cttaaaaagc tcaagtgctc
      601 caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc gctactctcc
      661 caaaaccaaa aggtctccgc tgactagggc acatctgaca gaagtggaat caaggctaga
      721 aagactggaa cagctatttc tactgatttt tcctcgagaa gaccttgaca tgattttgaa
      781 aatggattct ttacaggata taaaagcatt gttaacagga ttatttgtac aagataatgt
      841 gaataaagat gccgtcacag atagattggc ttcagtggag actgatatgc ctctaacatt
      901 gagacagcat agaataagtg cgacatcatc atcggaagag agtagtaaca aaggtcaaag
      961 acagttgact gtatcgaact atctattcga tgatgaagat accccaccaa acccaaaaaa
     1021 agagatcgaa ttccagctga ccaccatggc aattcccggg gatccgtcga cctgcagaga
     1081 tctatgaatc gtagatactg aaaaaccccg caagttcact tcaactgtgc atcgtgcacc
     1141 atctcaattt ctttcattta tacatcgttt tgccttcttt tatgtaacta tactcctcta
     1201 agtttcaatc ttggccatgt aacctctgat ctatagaatt ttttaaatga ctagaattaa
     1261 tgcccatctt ttttttggac ctaaattctt catgaaaata tattacgagg gcttattcag
     1321 aagctttgga cttcttcgcc agaggtttgg tcaagtctcc aatcaaggtt gtcggcttgt
     1381 ctaccttgcc agaaatttac gaaaagatgg aaaagggtca aatcgttggt agatacgttg
     1441 ttgacacttc taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta
     1501 taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct
     1561 tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg
     1621 ctcttattga ccacacctct accggcatgc cgagcaaatg cctgcaaatc gctccccatt
     1681 tcacccaatt gtagatatgc taactccagc aatgagttga tgaatctcgg tgtgtatttt
     1741 atgtcctcag aggacaacac ctgttgtaat cgttcttcca cacggatcct ggcgtaatag
     1801 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     1861 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca taatcgctgg
     1921 gccattctca tgaagaatat cttgaattta ttgtcatatt actagttggt gtggaagtcc
     1981 taatatcggt gatcaatata gtggttgaca tgctggctag tcaacattga gccttttgat
     2041 catgcaaata tattacggta ttttacaatc aaatatcaaa cttaactatt gactttataa
     2101 cttatttagg tggtaacatt cttataaaaa agaaaaaaat tactgcaaaa cagtactagc
     2161 ttttaacttg tatcctaggt tatctatgct gtctcaccat agagaatatt acctatttca
     2221 gaatgtatgt ccatgattcg ccgggtaaat acatataata cacaaatctg gcttaataaa
     2281 gtctataata tatctcataa agaagtgcta aattggctag tgctatatat ttttaagaaa
     2341 atttcttttg actaagtcca tatcgacttt gtaaaagttc acattagcat acatatatta
     2401 cacgagccag aaatagtaac ttttgcctaa atcacaaatt gcaaaattta attgcttgca
     2461 aaaggtcaca tgcttataat caactttttt aaaaatttaa aatacttttt tattttttat
     2521 ttttaaacat aaatgaaata atttatttat tgtttatgat taccgaaaca taaaacctgc
     2581 tcaagaaaaa gaaactgttt tgtccttgga aaaaaagcac tacctaggag cggccaaaat
     2641 gccgaggctt tcatagctta aactctttac agaaaatagg cattatagat cagttcgagt
     2701 tttcttattc ttccttccgg ttttatcgtc acagttttac agtaaataag tatcacctct
     2761 tagagttcga tgataagctg tcaaacatga gaattaattc cacatgttaa aatagtgaag
     2821 gagcatgttc ggcacacagt ggaccgaacg tggggtaagt gcactagggt ccggttaaac
     2881 ggatctcgca ttgatgaggc aacgctaatt atcaacatat agattgttat ctatctgcat
     2941 gaacacgaaa tctttacttg acgacttgag gctgatggtg tttatgcaaa gaaaccactg
     3001 tgtttaatat gtgtcactgt ttgatattac tgtcagcgta gaagataata gtaaaagcgg
     3061 ttaataagtg tatttgagat aagtgtgata aagtttttac agcgaaaaga cgataaatac
     3121 aagaaaatga ttacgaggat acggagagag gtatgtacat gtgtatttat atactaagct
     3181 gccggcggtt gtttgcaaga ccgagaaaag gctagcaaga atcgggtcat tgtagcgtat
     3241 gcgcctgtga acattctctt caacaagttt gattccattg cggtgaaatg gtaaaagtca
     3301 accccctgcg atgtatattt tcctgtacaa tcaatcaaaa agccaaatga tttagcatta
     3361 tctttacatc ttgttatttt acagatttta tgtttagatc ttttatgctt gcttttcaaa
     3421 aggcttgcag gcaagtgcac aaacaatact taaataaata ctactcagta ataacctatt
     3481 tcttagcatt tttgacgaaa tttgctattt tgttagagtc ttttacacca tttgtctcca
     3541 cacctccgct tacatcaaca ccaataacgc catttaatct aagcgcatca ccaacatttt
     3601 ctggcgtcag tccaccagct aacataaaat gtaagctctc ggggctctct tgccttccaa
     3661 cccagtcaga aatcgagttc caatccaaaa gttcacctgt cccacctgct tctgaatcaa
     3721 acaagggaat aaacgaatga ggtttctgtg aagctgcact gagtagtatg ttgcagtctt
     3781 ttggaaatac gagtctttta ataactggca aaccgaggaa ctcttggtat tcttgccacg
     3841 actcatctcc atgcagttgg acgatatcaa tgccgtaatc attgaccaga gccaaaacat
     3901 cctccttagg ttgattacga aacacgccaa ccaagtattt cggagtgcct gaactatttt
     3961 tatatgcttt tacaagactt gaaattttcc ttgcaataac cgggtcaatt gttctctttc
     4021 tattgggcac acatataata cccagcaagt cagcatcgga atctagtgca cattctgcgg
     4081 cctctgtgct ctgcaagccg caaactttca ccaatggacc agaactacct gtgaaattaa
     4141 taacagacat actccaagct gcctttgtgt gcttaatcac gtatactcac gtgctcaata
     4201 gtcaccaatg ccctccctct tggccctctc cttttctttt ttcgaccgaa ttaattcttg
     4261 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     4321 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4381 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4441 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4501 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4561 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4621 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4681 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4741 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4801 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4861 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4921 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4981 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5041 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5101 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5161 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5221 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5281 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5341 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5401 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5461 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5521 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5581 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5641 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5701 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5761 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5821 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5881 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5941 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6001 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6061 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6121 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6181 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     6241 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     6301 gccgattcat taatgcagga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc
     6361 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt
     6421 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca
     6481 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc
     6541 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg
     6601 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg
     6661 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa
     6721 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat
     6781 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg
     6841 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt
     6901 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac
     6961 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac
     7021 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt
     7081 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga
     7141 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat
     7201 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc
     7261 attgatg
//
