LOCUS       sequence-393061-        4876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive LuxR expression, controlled under E.coli hybrid 
            (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) 
            expression, controlled under V. fischeri HSL-regulated pLux (luxPR) 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Toparlak OD, Zasso J, Bridi S, Serra MD, Macchi P, Conti L, Baudet 
            ML, Mansy SS
  TITLE     Artificial cells drive neural differentiation.
  JOURNAL   Sci Adv. 2020 Sep 18;6(38). pii: 6/38/eabb4920. doi: 
            10.1126/sciadv.abb4920. Print 2020 Sep.
  PUBMED    32948587
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-09-18"; volume: "6(38). pii: 6/38/eabb4920. doi"; pages:
            " 10.1126/sciadv.abb4920. Print 2020 Sep"
COMMENT     Sequence Label: DT034A
FEATURES             Location/Qualifiers
     source          1..4876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(113..904,905..973))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     736..755
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(974..1078)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1146..1164
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1168..1211)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1214..1233
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     CDS             1298..1321
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      2196..2243
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             2336..2359
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      3984..4031
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    4033..4053
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(4059..4076)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4230..4818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4310..4329)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
       61 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
      121 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
      181 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
      241 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
      301 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
      361 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
      421 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
      481 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
      541 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
      601 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
      661 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
      721 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
      781 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
      841 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
      901 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
      961 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     1021 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     1081 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     1141 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta
     1201 aggatgattt ctggaattcg cggccgcttc tagatttacg gctagctcag tcctagggat
     1261 tgtgctagcg atactagaag aaggagaata atctatggac tacaaagacg atgatgacaa
     1321 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
     1381 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
     1441 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
     1501 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
     1561 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
     1621 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
     1681 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
     1741 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
     1801 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
     1861 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
     1921 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
     1981 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
     2041 aggagcaatt gattgcccat actttaaaaa ttaataacga ctcaggctgc tacgcctgtg
     2101 tactggaaaa caaaaccaaa acccaaaaaa caaaaaactg agcccattgg tatcgtggaa
     2161 ggactcgatc aaaaaaaaaa aaaaaaaaaa aaaaactagc ataacccctt ggggcctcta
     2221 aacgggtctt gaggggtttt ttggtactag aacctgtagg atcgtacagg tttacgcaag
     2281 aaaatggttt gttatagtcg aataaaaaat actagaagaa ggagaataat ctatggacta
     2341 caaagacgat gatgacaaga tgaaagatat cacggataaa aaccaatcga ttgatagtgg
     2401 catcagttcg ctgagctata accgcaatga agtcctggcc agcaatggtg ataaaattga
     2461 atcgtttgtt cctaaagagg gtaaaaaagc gggcaataaa ttcattgtag tcgaacgcca
     2521 gaaacgtagc ctgactactt caccggttga tatttccatt attgacagtg ttaatgatcg
     2581 cacgtatcct ggtgcgctgc aactggctga taaagcattt gtcgaaaatc gccctactat
     2641 tctgatggta aaacgtaaac cgatcaatat caacattgat ctgccgggtc tgaaaggtga
     2701 aaactctatc aaggtggatg atccaactta tggtaaagtc agcggcgcca ttgatgaact
     2761 ggttagcaaa tggaatgaaa aatatagcag cacccacacc ctgccggcgc gcacccagta
     2821 ctccgaatcc atggtgtata gtaaatccca aatctcatcg gccctgaatg tgaacgctaa
     2881 agtgctggaa aattcgctgg gtgttgactt caacgccgta gcaaataacg agaaaaaagt
     2941 aatgatcctg gcatataagc agatctttta tacagtcagt gccgatctgc cgaaaaatcc
     3001 gtctgatctg ttcgatgata gcgttacttt taatgatctg aagcaaaaag gtgttagcaa
     3061 cgaagctccg ccgctgatgg tttctaatgt tgcctatggt cgcaccattt atgttaaact
     3121 ggaaaccact agctcctcga aagatgtcca agctgctttt aaagctctga tcaaaaatac
     3181 agatattaaa aatagtcaac agtataaaga tatttatgaa aatagcagtt tcacagcagt
     3241 tgtgctggga ggggatgcac aggaacataa taaagtggtg acgaaagatt ttgacgaaat
     3301 ccgcaaagtc atcaaagata atgccacttt tagtacgaag aatccggctt atccaatcag
     3361 ctatacctct gtcttcctga aagacaacag cgttgccgcc gtccataaca agaccgatta
     3421 cattgaaacc accagtaccg aatattctaa aggtaaaatc aacctggatc attccggtgc
     3481 gtatgtcgct cagtttgaag tggcgtggga cgaggtctca tacgataaag aaggtaacga
     3541 agttctgacg cataaaactt gggacggcaa ttaccaggac aaaactgccc actatagcac
     3601 cgttatcccg ctggaagcca atgcgcgcaa catccgcatt aaagcacgtg aagcaacggg
     3661 gctggcctgg gaatggtggc gtgatgtaat tagcgaatat gatgtgccgc tgacgaataa
     3721 tatcaacgtt tcgatttggg ggaccaccct gtatccgggt tcaagcatta cctataatgc
     3781 ggcgaacaaa aacgaagaaa acaccaacga agtgccgacc tttatgctga acgcgggcca
     3841 ggcgaacaga agacgagttt gatgacgact caggctgcta cgcctgtgta ctggaaaaca
     3901 aaaccaaaac ccaaaaaaca aaaaactgag cccattggta tcgtggaagg actcgatcaa
     3961 aaaaaaaaaa aaaaaaaaaa aaactagcat aaccccttgg ggcctctaaa cgggtcttga
     4021 ggggtttttt ggtactagta gcggccgctg caggcttcct cgctcactga ctcgctgcgc
     4081 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     4141 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4201 aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat
     4261 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4321 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4381 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4441 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4501 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4561 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4621 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     4681 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4741 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4801 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4861 aacgaaaact cacgtt
//
