LOCUS       sequence-393088-        3488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with 
            scrambled anti-repeat sequencesequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Jiao C, Reckstadt C, Konig F, Homberger C, Yu J, Vogel J, Westermann
            AJ, Sharma CM, Beisel CL
  TITLE     RNA recording in single bacterial cells using reprogrammed 
            tracrRNAs.
  JOURNAL   Nat Biotechnol. 2023 Jan 5. doi: 10.1038/s41587-022-01604-8.
  PUBMED    36604543
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 5. doi: 10.1038/s41587-022-01604-8."
COMMENT     Sequence Label: tracrRNA(scr)-CJ8421_04975 mRNA
FEATURES             Location/Qualifiers
     source          1..3488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     129..148
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        566..600
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     primer_bind     complement(1832..1850)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1846..1893
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(join(2060..2851,2852..2920))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2683..2702
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2921..3025)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(3128..3488,1..228)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
       61 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      121 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      181 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      241 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
      301 ttatcccctg attctgtgga taaccgtgcg gcccgagagc atcgatccta gcatggatgc
      361 attgagctaa caccgtgcgt gttgacaatt ttacctctgg cggtgataat ggttgcatca
      421 cttcataatc gttcgcaatt cagggcttga cctacaaggc ttcatgccga aatcaacacc
      481 ctgtcatttt atggcagggt gttttcaaag cccgccgaaa ggcgggcttt tctgtaatac
      541 ctcgacactg ggatactgta ggatcttgac agctagctca gtcctaggta taatgctagc
      601 aatttccttc caagctccat ctatatcaaa ttcaagctct gttccatcgc taagatagac
      661 ttcataggaa tttttgtctt tttgcacgat ccctataggc gctttgaaat tatgttgtaa
      721 aaattcttta gaaacatttg gtaaattatc agcagaaatt attatatcag catttaaaga
      781 taaacttaaa atagtaataa tatttaaaaa tttaattttc aatatttttc ctaaattatt
      841 ttttagtaaa attgtaaaga atttatgtga attttatgtg aattttaaga caatattata
      901 aaaaataaag gttttatatt ttgcttacaa tttttttaat ttttttaaat attttatgtt
      961 attttttgat ttcttatgat tattacaata tccttggttt gaatatttta ttttttaatg
     1021 gagcttattg gcagctttta agttcaatgt ttttacatgg aaatttaact catcttatat
     1081 taaatatgat agttttgttt caatttgggc gtatgttaga aacttatttg ggtgctttgt
     1141 gttttatttt tatttatttt attggcggat tgttgtgctc acttttaagt gttttttatg
     1201 tgtattttga ttttaaatat tttggagaaa atataaatgt cataggcgct agtggtgcaa
     1261 tttgtgtttt aatgggcttt tatgctgtta ttgataaaaa tagcacaaaa ggacttatag
     1321 tggcaatttt acttatgagt tttgtgccat tgcttatggg gataaatgtt gcttggtatg
     1381 gtcatatttt cgggtttatg tgtggttata ttttagctaa aataaaagag gtaaaatgaa
     1441 aaaaatttat attataagac atgcaaaagc aagtaagagt gaagacatcg atgattttga
     1501 aagaaagctt actaagagtg gaaaggaaga tttaaaaaaa ctttttaaaa atcttgcttc
     1561 gcatgaaatt catcctgatt tggtgctatc aagtcctgct gttagaaccg ctaaaacagc
     1621 gaaaaaaata gccaaatttt ataattttga taaaaataga atttgttttg atgaaagatt
     1681 gtatctttgc aatgttgaaa atttgctaaa aattctgcaa gatattgatg atgagtttaa
     1741 tgaagtattt ttagtgggtc ataatcctag tttaatggag cttggagagc tgctaacaaa
     1801 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
     1861 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggataa
     1921 gcttgaaaga atcgatcgat cccgaatgag tagtacagcg gccgcgatta tcaaaaagga
     1981 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2041 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2101 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     2161 agggcttacc atctggcccc agtgctgcaa tgataccgcg ggacccacgc tcaccggctc
     2221 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2281 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2341 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2401 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2461 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2521 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2581 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2641 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2701 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2761 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2821 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     2881 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     2941 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3001 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gtcatgacca
     3061 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3121 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3181 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3241 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3301 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3361 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3421 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3481 gaacgacc
//
