LOCUS       sequence-393090-       10174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit 
            (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components 
            of a BRET2 biosensor for studying heterotrimeric G proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, 
            Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT
  TITLE     TRUPATH, an open-source biosensor platform for interrogating the 
            GPCR transducerome.
  JOURNAL   Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 
            10.1038/s41589-020-0535-8.
  PUBMED    32367019
REFERENCE   2  (bases 1 to 10174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 
            10.1038/s41589-020-0535-8."
COMMENT     Sequence Label: TRUPATH Triple Gagust
FEATURES             Location/Qualifiers
     source          1..10174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1937..1990
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3022..3039)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    3051..3624
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3074..3626
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3218..3235)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3445..3464
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(5765..5782)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5771..5995
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6041..6469
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6128..6147)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6338..6359
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6478..6498)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6483..6812
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6663..6798
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6725..6744
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6879..7673
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6933..6952)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7543..7562
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7847..7968
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7884..7903)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7938..7957
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8017..8033)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8017..8033)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8030..8052)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8041..8057
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8065..8071,8072..8089,8090..8095))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8110..8131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8248..8265)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8419..9007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8499..8518)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9178..9969,9970..10038))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9801..9820
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10039..10143)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgg gagagatgga gcagctgagg caggaggcag agcagctgaa
      961 gaagcagatc gccgacgcca gaaaggcctg cgcagatgtg accctggcag agctggtgag
     1021 cggactggag gtggtgggaa gggtgcagat gaggaccagg agaacactga ggggccacct
     1081 ggccaagatc tacgccatgc actgggccac agactctaag ctgctggtgt ccgcctctca
     1141 ggacggcaag ctgatcgtgt gggatagcta taccacaaac aaggtgcacg caatccccct
     1201 gcgcagctcc tgggtcatga cctgtgccta cgccccttcc ggaaatttcg tggcatgcgg
     1261 aggactggat aacatgtgct ctatctataa tctgaagagc cgggagggca acgtgaaggt
     1321 gtctagagag ctgagcgccc acaccggcta cctgtcttgc tgtcggttcc tggacgataa
     1381 caatatcgtg acatctagcg gcgacaccac atgcgcactg tgggacatcg agaccggaca
     1441 gcagaagaca gtgtttgtgg gccacacagg cgactgtatg tccctggccg tgtctccaga
     1501 tttcaatctg tttatctccg gagcatgcga cgcatctgcc aagctgtggg atgtgcggga
     1561 gggcacctgt agacagacct tcacaggcca cgagagcgac atcaacgcca tctgcttctt
     1621 tcccaatggc gaggccatct gcacaggcag cgacgatgcc tcctgtaggc tgttcgacct
     1681 gcgcgccgat caggagctga tctgcttttc tcacgagagc atcatctgtg gcatcacctc
     1741 cgtggccttt agcctgtccg gcaggctgct gttcgcagga tatgacgact tcaactgcaa
     1801 cgtgtgggac tccatgaagt ctgagagggt gggcatcctg agcggccacg ataatcgcgt
     1861 gtcctgtctg ggagtgaccg cagacggaat ggcagtggcc acaggcagct gggattcctt
     1921 tctgaagatc tggaacgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     1981 tcccggccct accggtatgg tgtccaaggg agaggagctg ttcaccggag tggtgcctat
     2041 cctggtggag ctggacggcg atgtgaatgg ccacaagttt agcgtgtccg gagagggaga
     2101 gggcgatgca acctacggca agctgacact gaagttcatc tgcaccacag gcaagctgcc
     2161 cgtgccttgg ccaaccctgg tgaccacact gtcctacggc gtgcagtgtt tttctcgcta
     2221 tcctgaccac atgaagcagc acgatttctt taagtctgcc atgccagagg gctacgtgca
     2281 ggagaggaca atcttcttta aggacgatgg caattataag acccgcgccg aggtgaagtt
     2341 cgagggcgat acactggtga accggatcga gctgaagggc atcgacttta aggaggatgg
     2401 caatatcctg ggccacaagc tggagtacaa ctataatccc cacaacgtgt atatcatggc
     2461 cgacaagcag aagaacggca tcaaggtcaa tttcaagatc agacacaaca tcgaggacgg
     2521 ctctgtgcag ctggccgatc actaccagca gaacacccca atcggcgacg gacccgtgct
     2581 gctgcctgat aatcactatc tgtttacaca gtctgccctg agcaaggacc ctaatgagaa
     2641 gagggatcac atggtgctgc tggagttcgt gaccgcagca ggcatcacac tgggcatgga
     2701 tgagctgtac aagggcagcg ccggcaccat gccagtgatc aacatcgagg acctgacaga
     2761 gaaggataag ctgaagatgg aggtggacca gctgaagaag gaggtgaccc tggagcggat
     2821 gctggtgagc aagtgctgtg aggagtttcg ggactatgtg gaggagagat ccggagagga
     2881 ccccctggtg aagggcatcc cagaggacaa gaaccccttc aaggagctga agggcggctg
     2941 cgtgatcagc tgaccgcggc gtacgaggcc tgcatgctcc ggacctgcag gttcgaagtc
     3001 gacagatctc aattggggcc cctatagtgt cacctaaata attccgcccc cccctctccc
     3061 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
     3121 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
     3181 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
     3241 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
     3301 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
     3361 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
     3421 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
     3481 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
     3541 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
     3601 ttcctttgaa aaacacgatg ataatatggg cagcggcatc agctccgagt ccaaggagtc
     3661 tgccaagagg tccaaggagc tggagaagaa gctgcaggag gacgcagaga gggatgcaag
     3721 aaccgtgaag ctgctgctgc tgggagcagg agagagcggc aagtccacaa tcgtgaagca
     3781 gatgaagatc atccacaaga acggctacag cgagcaggag tgcatggagt tcaaggccgt
     3841 gatctattct aataccctgc agagcatcct ggccatcgtg aaggccatga ccacactggg
     3901 catcgattac gtgaacccaa ggtccgcaga ggaccagagg cagctgtatg ccatggccaa
     3961 taccctggag gattctggag gaggaggctc tatggcaagc aaggtgtacg acccagagca
     4021 gaggaagcgc atgatcacag gaccacagtg gtgggcaagg tgcaagcaga tgaacgtgct
     4081 ggattccttt atcaattact atgactctga gaagcacgcc gagaacgccg tgatcttcct
     4141 gcacggcaat gccacctcta gctacctgtg gaggcacgtg gtgccccaca tcgagcctgt
     4201 ggcaaggtgt atcatccctg atctgatcgg catgggcaag tccggcaagt ctggcaacgg
     4261 ctcctatagg ctgctggacc actacaagta tctgacagcc tggttcgagc tgctgaatct
     4321 gccaaagaag atcatctttg tgggacacga ttggggagca gcactggcat tccactacgc
     4381 atatgagcac caggatcgca tcaaggccat cgtgcacatg gagagcgtgg tggacgtgat
     4441 cgagtcctgg gacgagtggc ctgacatcga ggaggacatc gccctgatca agagcgagga
     4501 gggcgagaag atggtgctgg agaacaattt ctttgtggag accgtgctgc catccaagat
     4561 catgaggaag ctggagccag aggagttcgc agcatacctg gagcctttta aggagaaggg
     4621 cgaggtgcgg agacccacac tgtcttggcc acgcgagatc ccactggtga agggaggcaa
     4681 gcctgatgtg gtgcagatcg tgcggaacta caatgcctat ctgagagcca gcgacgatct
     4741 gcccaagctg tttatcgaga gcgaccctgg cttcttttcc aacgccatcg tggagggcgc
     4801 caagaagttc ccaaataccg agtttgtgaa ggtgaagggc ctgcactttc tgcaggagga
     4861 cgcccccgat gagatgggca agtatatcaa gagcttcgtg gagcgggtgc tgaagaacga
     4921 gcagagcgga ggaggaggct ctggaggcat gacacctcag ctggccgaag tgatcaagag
     4981 gctgtggcgc gatccaggca tccaggcctg cttcgagaga gcctctgagt accagctgaa
     5041 cgacagcgcc gcctactatc tgaatgacct ggatcggatc accgcctccg gctatgtgcc
     5101 caacgagcag gacgtgctgc actctagagt gaagaccaca ggcatcatcg agacacagtt
     5161 ctcttttaag gatctgcact tccggatgtt tgacgtgggc ggccagcgga gcgagagaaa
     5221 gaagtggatt cactgtttcg agggcgtgac ctgcatcatc ttttgtgccg ccctgtccgc
     5281 ctacgatatg gtgctggtgg aggacgagga ggtgaacaga atgcacgagt ctctgcacct
     5341 gttcaacagc atctgcaatc acaagtattt tagcaccaca tccatcgtgc tgttcctgaa
     5401 taagaaggac atctttcagg agaaggtgac caaggtgcac ctgtccatct gtttccctga
     5461 gtacaccggc ccaaacacat ttgaggacgc cggcaactat atcaagaatc agtttctgga
     5521 tctgaatctg aagaaggagg acaaggagat ctactctcac atgacctgcg ccaccgatac
     5581 acagaacgtg aagttcgtgt ttgatgccgt gacagacatc atcatcaagg agaatctgaa
     5641 ggactgtggc ctgttctgag gtaccgagct cggatccact agtccagtgt ggtggaattc
     5701 tgcagatatc cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga
     5761 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     5821 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     5881 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     5941 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     6001 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     6061 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6121 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6181 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6241 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6301 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6361 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6421 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     6481 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6541 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     6601 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6661 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6721 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     6781 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     6841 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     6901 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     6961 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     7021 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     7081 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     7141 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     7201 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     7261 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     7321 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     7381 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     7441 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     7501 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     7561 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     7621 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     7681 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     7741 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     7801 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     7861 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7921 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     7981 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8041 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8101 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8161 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8221 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8281 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8341 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8401 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8461 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8521 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8581 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8641 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8701 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8761 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8821 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     8881 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8941 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9001 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9061 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9121 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9181 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9241 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9301 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9361 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9421 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9481 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9541 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9601 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9661 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9721 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9781 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9841 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9901 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9961 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10021 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10081 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10141 gcgcacattt ccccgaaaag tgccacctga cgtc
//
