LOCUS       sequence-393091-        8496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of truncated HSF1 (DBD and HR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Price RM, Budzynski MA, Shen J, Mitchell JE, Kwan JZJ, Teves SS
  TITLE     Heat shock transcription factors demonstrate a distinct mode of 
            interaction with mitotic chromosomes.
  JOURNAL   Nucleic Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304.
  PUBMED    37114996
REFERENCE   2  (bases 1 to 8496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304."
COMMENT     Sequence Label: H1DBD-HR-Halo
FEATURES             Location/Qualifiers
     source          1..8496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        377..588
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             601..869
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             1588..1608
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             1618..2508
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             2509..2532
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2557..3129
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2724..2741)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2951..2970
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3138..3932
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3192..3211)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3802..3821
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3992..4012)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    4054..4175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4091..4110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4145..4164
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4938..4972
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5463..5479)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5463..5479)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5476..5498)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5487..5503
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5511..5517,5518..5535,5536..5541))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5556..5577
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5694..5711)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5865..6453)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5945..5964)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6624..7415,7416..7484))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7247..7266
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7485..7589)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7615..8070)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7752..7773)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7964..7983
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8197..8219
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8211..8228
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8212..8228
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   8358..8420
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 tactaataat aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaacaa
       61 aaactcaaaa tttcttctat aaagtaacaa aacttttatg agggacagcc cccccccaaa
      121 gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg
      181 ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc
      241 gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag
      301 cctgcagaca cctgggggga tacggggaaa aggcctccac ggccaaggat ctgcgatcgc
      361 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
      421 ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
      481 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
      541 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga
      601 ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg
      661 ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag
      721 gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct
      781 agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt
      841 tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac tatggatctg
      901 gccgtgggcc ccggtgcagc ggggcccagc aacgtcccgg ccttcctaac caagctgtgg
      961 accctcgtga gcgacccgga cacagacgcg ctcatctgct ggagcccgag tgggaacagc
     1021 ttccacgtgt ttgaccaggg ccagtttgcc aaggaggtgc tgcccaagta cttcaagcac
     1081 aacaacatgg ctagcttcgt gcggcagctc aacatgtatg gcttccgaaa agtagtccac
     1141 attgagcagg gtggcctggt caagcctgag agagatgaca ccgagttcca gcatccttgt
     1201 ttcttgcgtg gacaggaaca gctccttgag aacatcaaga ggaaagtgac cagcgtgtcc
     1261 accctgaaga gtgaggacat aaaaatacgc caggacagtg tcacccggct gttgacagat
     1321 gtgcagctga tgaaggggaa acaggagtgt atggactcca agctcctggc catgaagcac
     1381 gagaacgagg ccctgtggcg ggaggtggcc agccttcggc agaagcatgc ccagcagcaa
     1441 aaagttgtca acaagctcat tcagttcctg atctcactgg tgcagtcgaa ccggatcctg
     1501 ggggtgaaga gaaagatccc tctgatgttg agtgacagca actcagcaca ctctgtgccc
     1561 aagaattcct cgaggactag tcctcgagag gatctgtact ttcagagcgg aggctccatg
     1621 gcagaaatcg gtactggctt tccattcgac ccccattatg tggaagtcct gggcgagcgc
     1681 atgcactacg tcgatgttgg tccgcgcgat ggcacccctg tgctgttcct gcacggtaac
     1741 ccgacctcct cctacgtgtg gcgcaacatc atcccgcatg ttgcaccgac ccatcgctgc
     1801 attgctccag acctgatcgg tatgggcaaa tccgacaaac cagacctggg ttatttcttc
     1861 gacgaccacg tccgcttcat ggatgccttc atcgaagccc tgggtctgga agaggtcgtc
     1921 ctggtcattc acgactgggg ctccgctctg ggtttccact gggccaagcg caatccagag
     1981 cgcgtcaaag gtattgcatt tatggagttc atccgcccta tcccgacctg ggacgaatgg
     2041 ccagaatttg cccgcgagac cttccaggcc ttccgcacca ccgacgtcgg ccgcaagctg
     2101 atcatcgatc agaacgtttt tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg
     2161 actgaagtcg agatggacca ttaccgcgag ccgttcctga atcctgttga ccgcgagcca
     2221 ctgtggcgct tcccaaacga gctgccaatc gccggtgagc cagcgaacat cgtcgcgctg
     2281 gtcgaagaat acatggactg gctgcaccag tcccctgtcc cgaagctgct gttctggggc
     2341 accccaggcg ttctgatccc accggccgaa gccgctcgcc tggccaaaag cctgcctaac
     2401 tgcaaggctg tggacatcgg cccgggtctg aatctgctgc aagaagacaa cccggacctg
     2461 atcggcagcg agatcgcgcg ctggctgtcg acgctcgaga tttccggcga ctacaaagac
     2521 gatgacgaca agcgtacgta aggatccgcg gccgcgcccc tctccctccc ccccccctaa
     2581 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     2641 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     2701 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     2761 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     2821 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     2881 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     2941 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     3001 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     3061 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     3121 acgataatac ctccggaatg attgaacaag atggattgca cgcaggttct ccggccgctt
     3181 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     3241 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3301 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc gcgacgggcg
     3361 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3421 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3481 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3541 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3601 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3661 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3721 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3781 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3841 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3901 ccttctatcg ccttcttgac gagttcttct gagtcgacaa tcaacctctg gattacaaaa
     3961 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4021 ctgctttaat gcctttgtat catgcgttaa ctaaacttgt ttattgcagc ttataatggt
     4081 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4141 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggaattg actcaaatga
     4201 tgtcaattag tctatcagaa gctcatctgg tctcccttcc gggggacaag acatccctgt
     4261 ttaatattta aacagcagtg ttcccaaact gggttcttat atcccttgct ctggtcaacc
     4321 aggttgcagg gtttcctgtc ctcacaggaa cgaagtccct aaagaaacag tggcagccag
     4381 gtttagcccc ggaattgact ggattccttt tttagggccc attggtatgg ctttttcccc
     4441 gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga ggaaagcgat
     4501 cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg gggatgcggg
     4561 gggagcgccg gaccggagcg gagccccggg cggctcgctg ctgcccccta gcgggggagg
     4621 gacgtaatta catccctggg ggctttgggg gggggctgtc cctgatatct ataacaagaa
     4681 aatatatata taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta
     4741 tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc
     4801 gttatagttc aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca
     4861 agcggcgact gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga
     4921 gcaatatttc aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaatcta
     4981 gctgcatcag gatcatatcg tcgggtcttt tttccggctc agtcatcgcc caagctggcg
     5041 ctatctgggc atcggggagg aagaagcccg tgccttttcc cgcgaggttg aagcggcatg
     5101 gaaagagttt gccgaggatg actgctgctg cattgacgtt gagcgaaaac gcacgtttac
     5161 catgatgatt cgggaaggtg tggccatgca cgcctttaac ggtgaactgt tcgttcaggc
     5221 cacctgggat accagttcgt cgcggctttt ccggacacag ttccggatgg tcagcccgaa
     5281 gcgcatcagc aacccgaaca ataccggcga cagccggaac tgccgtgccg gtgtgcagat
     5341 taatgacagc ggtgcggcgc tgggatatta cgtcagcgag gacgggtatc ctggctggat
     5401 gccgcagaaa tggacatgga taccccgtga gttacccggc gggcgcgctt ggcgtaatca
     5461 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     5521 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     5581 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     5641 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5701 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5761 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5821 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5881 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5941 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6001 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6061 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6121 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6181 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6241 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6301 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6361 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6421 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6481 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6541 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6601 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6661 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6721 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6781 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6841 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6901 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6961 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7021 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7081 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7141 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7201 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7261 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7321 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7381 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7441 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7501 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7561 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     7621 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     7681 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     7741 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     7801 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     7861 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     7921 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     7981 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     8041 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     8101 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     8161 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     8221 cggccagtga gcgcgcctcg ttcattcacg tttttgaacc cgtggaggac gggcagactc
     8281 gcggtgcaaa tgtgttttac agcgtgatgg agcagatgaa gatgctcgac acgctgcaga
     8341 acacgcagct agattaaccc tagaaagata atcatattgt gacgtacgtt aaagataatc
     8401 atgtgtaaaa ttgacgcatg tgttttatcg gtctgtatat cgaggtttat ttattaattt
     8461 gaatagatat taagttttat tatatttaca cttaca
//
