LOCUS       sequence-393092-        9449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HSF2 with HSF1 DBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   Price RM, Budzynski MA, Shen J, Mitchell JE, Kwan JZJ, Teves SS
  TITLE     Heat shock transcription factors demonstrate a distinct mode of 
            interaction with mitotic chromosomes.
  JOURNAL   Nucleic Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304.
  PUBMED    37114996
REFERENCE   2  (bases 1 to 9449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304."
COMMENT     Sequence Label: Halo-H1DBD-H2
FEATURES             Location/Qualifiers
     source          1..9449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     481..500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(719..823)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(849..1304)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(986..1007)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1198..1217
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1431..1453
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1445..1462
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1446..1462
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1592..1654
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2107..2317
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2330..2598
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2624..2633
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2633..2698
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2708..3598
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             3611..3631
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     misc_feature    5240..5812
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5407..5424)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5634..5653
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5821..6615
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5875..5894)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6485..6504
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6675..6695)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6737..6858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6774..6793)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6828..6847
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7621..7655
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8146..8162)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8146..8162)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8159..8181)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8170..8186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8194..8200,8201..8218,8219..8224))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8239..8260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8377..8394)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8548..9136)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8628..8647)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9307..9449,1..649,650..718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
       61 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
      121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
      181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
      241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
      301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
      361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
      421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
      481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
      541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
      601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
      661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
      721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
      781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
      841 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      901 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      961 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     1021 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     1081 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     1141 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     1201 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     1261 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     1321 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     1381 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     1441 acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt cacgtttttg aacccgtgga
     1501 ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg atggagcaga tgaagatgct
     1561 cgacacgctg cagaacacgc agctagatta accctagaaa gataatcata ttgtgacgta
     1621 cgttaaagat aatcatgtgt aaaattgacg catgtgtttt atcggtctgt atatcgaggt
     1681 ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca tactaataat
     1741 aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaacaa aaactcaaaa
     1801 tttcttctat aaagtaacaa aacttttatg agggacagcc cccccccaaa gcccccaggg
     1861 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     1921 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     1981 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     2041 cctgggggga tacggggaaa aggcctccac ggccaaggat ctgcgatcgc tccggtgccc
     2101 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     2161 attgaacggg tgcctagaga aggtgcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2221 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2281 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
     2341 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
     2401 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
     2461 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
     2521 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
     2581 tctgcgccgt tacagatcca agctgtgacc ggcgcctact ctagccacca tggactacaa
     2641 agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg atgacaagct
     2701 tggctccatg gcagaaatcg gtactggctt tccattcgac ccccattatg tggaagtcct
     2761 gggcgagcgc atgcactacg tcgatgttgg tccgcgcgat ggcacccctg tgctgttcct
     2821 gcacggtaac ccgacctcct cctacgtgtg gcgcaacatc atcccgcatg ttgcaccgac
     2881 ccatcgctgc attgctccag acctgatcgg tatgggcaaa tccgacaaac cagacctggg
     2941 ttatttcttc gacgaccacg tccgcttcat ggatgccttc atcgaagccc tgggtctgga
     3001 agaggtcgtc ctggtcattc acgactgggg ctccgctctg ggtttccact gggccaagcg
     3061 caatccagag cgcgtcaaag gtattgcatt tatggagttc atccgcccta tcccgacctg
     3121 ggacgaatgg ccagaatttg cccgcgagac cttccaggcc ttccgcacca ccgacgtcgg
     3181 ccgcaagctg atcatcgatc agaacgtttt tatcgagggt acgctgccga tgggtgtcgt
     3241 ccgcccgctg actgaagtcg agatggacca ttaccgcgag ccgttcctga atcctgttga
     3301 ccgcgagcca ctgtggcgct tcccaaacga gctgccaatc gccggtgagc cagcgaacat
     3361 cgtcgcgctg gtcgaagaat acatggactg gctgcaccag tcccctgtcc cgaagctgct
     3421 gttctggggc accccaggcg ttctgatccc accggccgaa gccgctcgcc tggccaaaag
     3481 cctgcctaac tgcaaggctg tggacatcgg cccgggtctg aatctgctgc aagaagacaa
     3541 cccggacctg atcggcagcg agatcgcgcg ctggctgtcg acgctcgaga tttccggcga
     3601 gccaaccact gaggatctgt actttcagag cgataactct agaatggatc tggccgtggg
     3661 ccccggtgca gcggggccca gcaacgtccc ggccttccta accaagctgt ggaccctcgt
     3721 gagcgacccg gacacagacg cgctcatctg ctggagcccg agtgggaaca gcttccacgt
     3781 gtttgaccag ggccagtttg ccaaggaggt gctgcccaag tacttcaagc acaacaacat
     3841 ggctagcttc gtgcggcagc tcaacatgta tggcttccga aaagtagtcc acattgagca
     3901 gggtggcctg gtcaagcctg agagagatga caccgagttc cagcatcctt gtttcttgcg
     3961 tggacaggaa cagctccttg agaacatcaa gaggaaagtg accagcgtgt ccaccctgaa
     4021 gagtgaggac ataaaaatac gccaggaaga tttaacaaaa attattagta gtgctcagaa
     4081 ggttcaaata aaacaagaaa ctattgagtc caggctttca gaattaaaaa gtgagaatga
     4141 atccctttgg aaggaggtgt cagaactaag agcaaagcat gcccagcagc aacaagttat
     4201 tcggaagatt gtccagttta ttgttacatt ggttcagaat aatcaacttg tgagtttaaa
     4261 acgtaaaagg cctctacttc taaacacaaa tggagcccca aagaagaatc tatatcagca
     4321 catagtcaaa gaaccaactg ataatcacca tcataaagtt ccacacagca ggactgaagg
     4381 tttaaagtca agagaacgga tttcagatga cataattatt tatgatgtta ctgacgataa
     4441 tgtggatgaa gaaaatattc cagttattcc agaaacaaat gaggatgttg tagtggattc
     4501 ctccaaccag tatcctgaca ttgtcattgt tgaagatgac aacgaggatg agtatgctcc
     4561 tgtcattcag agtggagagc agagtgaacc agccagagaa cccttacgtg tggggagtgc
     4621 tggcagcagc agccctctca tgtctagtgc tgtccagcta aacggctcct ccagtctgac
     4681 ctcagaagac cctgtgacca tgatggactc cattctgaat gacaacatta acctgttagg
     4741 aaaggttgag ctgttggatt accttgacag tattgattgc agtttagagg acttccaagc
     4801 tatgctctca ggaagacagt ttagcataga cccagatctt ctggttgatt ctgagaataa
     4861 gggactagaa gctaccaaga gcagtgttgt tcaacatgtg tcagaagagg gaagaaaatc
     4921 taaatccaag ccagacaaac aacttatcca gtatactgcc tttccacttc ttgcattcct
     4981 ggatgggaac tctgcatctg ctattgaaca ggggagtaca actgcatcgt cagaagttgt
     5041 gccttctgta gataaaccca tagaagtcga tgagctcctg gatagcagcc tggatccaga
     5101 accgacccag agtaagcttg tccgcctgga accattgact gaagcggaag ctagtgaagc
     5161 cacactcttc tatttatgtg aacttgctcc tgcacctctg gatagtgata tgccgctttt
     5221 agatagttaa gcggccgcgc ccctctccct cccccccccc taacgttact ggccgaagcc
     5281 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     5341 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     5401 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     5461 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     5521 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     5581 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     5641 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     5701 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     5761 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgataa tacctccgga
     5821 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     5881 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     5941 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     6001 caggacgagg cagcgcggct atcgtggctg gccgcgacgg gcgttccttg cgcagctgtg
     6061 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     6121 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     6181 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     6241 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     6301 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     6361 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     6421 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     6481 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     6541 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     6601 gacgagttct tctgagtcga caatcaacct ctggattaca aaatttgtga aagattgact
     6661 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6721 tatcatgcgt taactaaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6781 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6841 actcatcaat gtatcttatc atgtctggaa ttgactcaaa tgatgtcaat tagtctatca
     6901 gaagctcatc tggtctccct tccgggggac aagacatccc tgtttaatat ttaaacagca
     6961 gtgttcccaa actgggttct tatatccctt gctctggtca accaggttgc agggtttcct
     7021 gtcctcacag gaacgaagtc cctaaagaaa cagtggcagc caggtttagc cccggaattg
     7081 actggattcc ttttttaggg cccattggta tggctttttc cccgtatccc cccaggtgtc
     7141 tgcaggctca aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc
     7201 gtgcccgggc tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga
     7261 gcggagcccc gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct
     7321 gggggctttg ggggggggct gtccctgata tctataacaa gaaaatatat atataataag
     7381 ttatcacgta agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa
     7441 gtcacgtaaa agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca
     7501 gtgacactta ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt
     7561 cctaaatgca cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg
     7621 catgcgtcaa ttttacgcag actatctttc tagggttaat ctagctgcat caggatcata
     7681 tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg
     7741 aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag tttgccgagg
     7801 atgactgctg ctgcattgac gttgagcgaa aacgcacgtt taccatgatg attcgggaag
     7861 gtgtggccat gcacgccttt aacggtgaac tgttcgttca ggccacctgg gataccagtt
     7921 cgtcgcggct tttccggaca cagttccgga tggtcagccc gaagcgcatc agcaacccga
     7981 acaataccgg cgacagccgg aactgccgtg ccggtgtgca gattaatgac agcggtgcgg
     8041 cgctgggata ttacgtcagc gaggacgggt atcctggctg gatgccgcag aaatggacat
     8101 ggataccccg tgagttaccc ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc
     8161 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8221 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8281 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8341 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8401 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8461 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8521 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8581 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8641 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8701 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8761 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8821 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8881 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8941 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     9001 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9061 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9121 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9181 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9241 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9301 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9361 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9421 tggccccagt gctgcaatga taccgcgag
//
