LOCUS       sequence-393100-        6080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses AAV2 Rep and AAV4.52 Cap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Gonzalez TJ, Mitchell-Dick A, Blondel LO, Fanous MM, Hull JA, Oh DK,
            Moller-Tank S, Castellanos Rivera RM, Piedrahita JA, Asokan A
  TITLE     Structure-guided AAV capsid evolution strategies for enhanced CNS 
            gene delivery.
  JOURNAL   Nat Protoc. 2023 Sep 21. doi: 10.1038/s41596-023-00875-y.
  PUBMED    37735235
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2023 Sep 21. doi: 10.1038/s41596-023-00875-y."
COMMENT     Sequence Label: pAAV4.52-mut
FEATURES             Location/Qualifiers
     source          1..6080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(4331..4919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4411..4430)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5090..5881,5882..5950))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5713..5732
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5951..6055)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatatcctta aggtcctgta ttagaggtca cgtgagtgtt ttgcgacatt ttgcgacacc
       61 atgtggtcac gctgggtatt taagcccgag tgagcacgca gggtctccat tttgaagcgg
      121 gaggtttgaa cgcgcagcca ccacgccggg gttttacgag attgtgatta aggtccccag
      181 cgaccttgac gagcatctgc ccggcatttc tgacagcttt gtgaactggg tggccgagaa
      241 ggaatgggag ttgccgccag attctgacat ggatctgaat ctgattgagc aggcacccct
      301 gaccgtggcc gagaagctgc agcgcgactt tctgacggaa tggcgccgtg tgagtaaggc
      361 cccggaggcc cttttctttg tgcaatttga gaagggagag agctacttcc acatgcacgt
      421 gctcgtggaa accaccgggg tgaaatccat ggttttggga cgtttcctga gtcagattcg
      481 cgaaaaactg attcagagaa tttaccgcgg gatcgagccg actttgccaa actggttcgc
      541 ggtcacaaag accagaaatg gcgccggagg cgggaacaag gtggtggatg agtgctacat
      601 ccccaattac ttgctcccca aaacccagcc tgagctccag tgggcgtgga ctaatatgga
      661 acagtattta agcgcctgtt tgaatctcac ggagcgtaaa cggttggtgg cgcagcatct
      721 gacgcacgtg tcgcagacgc aggagcagaa caaagagaat cagaatccca attctgatgc
      781 gccggtgatc agatcaaaaa cttcagccag gtacatggag ctggtcgggt ggctcgtgga
      841 caaggggatt acctcggaga agcagtggat ccaggaggac caggcctcat acatctcctt
      901 caatgcggcc tccaactcgc ggtcccaaat caaggctgcc ttggacaatg cgggaaagat
      961 tatgagcctg actaaaaccg cccccgacta cctggtgggc cagcagcccg tggaggacat
     1021 ttccagcaat cggatttata aaattttgga actaaacggg tacgatcccc aatatgcggc
     1081 ttccgtcttt ctgggatggg ccacgaaaaa gttcggcaag aggaacacca tctggctgtt
     1141 tgggcctgca actaccggga agaccaacat cgcggaggcc atagcccaca ctgtgccctt
     1201 ctacgggtgc gtaaactgga ccaatgagaa ctttcccttc aacgactgtg tcgacaagat
     1261 ggtgatctgg tgggaggagg ggaagatgac cgccaaggtc gtggagtcgg ccaaagccat
     1321 tctcggagga agcaaggtgc gcgtggacca gaaatgcaag tcctcggccc agatagaccc
     1381 gactcccgtg atcgtcacct ccaacaccaa catgtgcgcc gtgattgacg ggaactcaac
     1441 gaccttcgaa caccagcagc cgttgcaaga ccggatgttc aaatttgaac tcacccgccg
     1501 tctggatcat gactttggga aggtcaccaa gcaggaagtc aaagactttt tccggtgggc
     1561 aaaggatcac gtggttgagg tggagcatga attctacgtc aaaaagggtg gagccaagaa
     1621 aagacccgcc cccagtgacg cagatataag tgagcccaaa cgggtgcgcg agtcagttgc
     1681 gcagccatcg acgtcagacg cggaagcttc gatcaactac gcagacaggt accaaaacaa
     1741 atgttctcgt cacgtgggca tgaatctgat gctgtttccc tgcagacaat gcgagagaat
     1801 gaatcagaat tcaaatatct gcttcactca cggacagaaa gactgtttag agtgctttcc
     1861 cgtgtcagaa tctcaacccg tttctgtcgt caaaaaggcg tatcagaaac tgtgctacat
     1921 tcatcatatc atgggaaagg tgccagacgc ttgcactgcc tgcgatctgg tcaatgtgga
     1981 tttggatgac tgcatctttg aacaataaat gatttaaata tggctgctga cggttacctt
     2041 ccagattggc tagaggacaa cctctctgaa ggcgttcgag agtggtgggc gctgcaacct
     2101 ggagccccta aacccaaggc aaatcaacaa catcaggaca acgctcgggg tcttgtgctt
     2161 ccgggttaca aatacctcgg acccggcaac ggactcgaca agggggaacc cgtcaacgca
     2221 gcggacgcgg cagccctcga gcacgacaag gcctacgacc agcagctcaa ggccggtgac
     2281 aacccctacc tcaagtacaa ccacgccgac gcggagttcc agcagcggct tcagggcgac
     2341 acatcgtttg ggggcaacct cggcagagca gtcttccagg ccaaaaagag ggttcttgaa
     2401 cctcttggtc tggttgagca agcgggtgag acggctcctg gaaagaagag accgttgatt
     2461 gaatcccccc agcagcccga ctcctccacg ggtatcggca aaaaaggcaa gcagccggct
     2521 aaaaagaagc tcgttttcga agacgaaact ggagcaggcg acggaccccc tgagggatca
     2581 acttccggag ccatgtctga tgacagtgag atgcgtgcag cagctggcgg agctgcagtc
     2641 gagggcggac aaggtgccga tggagtgggt aatgcctcgg gtgattggca ttgcgattcc
     2701 acctggtctg agggccacgt cacgaccacc agcaccagaa cctgggtctt gcccacctac
     2761 aacaaccacc tctacaagcg actcggagag agcctgcagt ccaacaccta caacggattc
     2821 tccaccccct ggggatactt tgacttcaac cgcttccact gccacttctc accacgtgac
     2881 tggcagcgac tcatcaacaa caactggggc atgcgaccca aagccatgcg ggtcaaaatc
     2941 ttcaacatcc aggtcaagga ggtcacgacg tcgaacggcg agacaacggt ggctaataac
     3001 cttaccagca cggttcagat ctttgcggac tcgtcgtacg aactgccgta cgtgatggat
     3061 gcgggtcaag agggcagcct gcctcctttt cccaacgacg tctttatggt gccccagtac
     3121 ggctactgtg gactggtgac cggcaacact tcgcagcaac agactgacag aaatgccttc
     3181 tactgcctgg agtactttcc ttcgcagatg ctgcggactg gcaacaactt tgaaattacg
     3241 tacagttttg agaaggtgcc tttccactcg atgtacgcgc acagccagag cctggaccgg
     3301 ctgatgaacc ctctcatcga ccagtacctg tggggactgc aatcgaccac caccggaacc
     3361 accctgaatg ccgggactgc caccaccaac tttaccaagc tgcggcctac caacttttcc
     3421 aactttaaaa agaactggct gcccgggcct tcaatcaagc agcagggctt ctcaaagact
     3481 gccaatcaaa actacgagat ccctgccacc gggtcagaca gtctcatcga atacgagacg
     3541 cacagcactc tggacggaag atggagtgcc ctgacccccg gacctccaat ggccacggct
     3601 ggacctgcgg acagcaagtt cagcaacagc cagctcatct ttgcggggcc taaacagaac
     3661 ggcaacacgg ccaccgtacc cgggactctg atcttcacct ctgaggagga gctggcagcc
     3721 accaacgcca ccgatacgga catgtggggc aacctacctg gcggtgacca gagcgccggc
     3781 ttcaggccga ccgtggacag actgacagcc ttgggagccg tgcctggaat ggtctggcaa
     3841 aacagagaca tttactacca gggtcccatt tgggccaaga ttcctcatac cgatggacac
     3901 tttcacccct caccgctgat tggtgggttt gggctgaaac acccgcctcc tcaaattttt
     3961 atcaagaaca ccccggtacc tgcgaatcct gcaacgacct tcagctctac tccggtaaac
     4021 tccttcatta ctcagtacag cactggccag gtgtcggtgc agattgactg ggagatccag
     4081 aaggagcggt ccaaacgctg gaaccccgag gtccagttta cctccaacta cggacagcaa
     4141 aactctctgt tgtgggctcc cgatgcggct gggaaataca ctgagcctag ggctatcggt
     4201 acccgctacc tcacccacca cctgtaataa cctgttaatc aataaaccgg tttattcgtt
     4261 tcagttgaac tttggtctag agatatcagc aaaaggccag gaaccgtaaa aaggccgcgt
     4321 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4381 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4441 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4501 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4561 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4621 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4681 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4741 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     4801 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4861 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4921 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4981 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     5041 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     5101 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctggc
     5161 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     5221 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     5281 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     5341 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5401 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5461 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5521 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5581 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5641 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5701 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5761 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5821 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5881 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5941 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     6001 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     6061 ttccccgaaa agtgccacct
//
