LOCUS       sequence-393105-       10806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, 
            and TniQ from a CMV promoter, in a polycistronic vector format 
            separated by T2A sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Lampe GD, King RT, Halpin-Healy TS, Klompe SE, Hogan MI, Vo PLH, 
            Tang S, Chavez A, Sternberg SH
  TITLE     Targeted DNA integration in human cells without double-strand breaks
            using CRISPR-associated transposases.
  JOURNAL   Nat Biotechnol. 2023 Mar 29. doi: 10.1038/s41587-023-01748-1.
  PUBMED    36991112
REFERENCE   2  (bases 1 to 10806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Mar 29. doi: 10.1038/s41587-023-01748-1."
COMMENT     Sequence Label: pSL3718 (pQCascade_Pse)
FEATURES             Location/Qualifiers
     source          1..10806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(104..208)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(283..302)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        474..853
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        854..1057
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1008..1028
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1102..1121
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1102..1120
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1156..1165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2254..2274
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2284..2337
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4471..4491
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4501..4554
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5212..5232
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5242..5295
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5332..5352
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6636..6653)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6642..6866
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6912..7340
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6999..7018)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7209..7230
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7349..7369)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7354..7683
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7534..7669
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7596..7615
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7750..8544
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7804..7823)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8414..8433
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8718..8839
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8755..8774)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8809..8828
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8888..8904)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8888..8904)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8901..8923)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8912..8928
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8936..8942,8943..8960,8961..8966))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8981..9002
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9119..9136)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9290..9878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9370..9389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10049..10806,1..34,35..103))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10672..10691
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
       61 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
      121 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
      181 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
      241 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
      301 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      361 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      421 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      481 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      541 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      601 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      661 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      721 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      781 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      841 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      901 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      961 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     1021 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
     1081 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcg
     1141 tttaaactta agcttgccac catggagctg tgcaacatcc tgaagtacga caggtctctg
     1201 tatcccggca aggccgtgtt cttttacaag accgccgaca gcgattttgt gccactggag
     1261 gccgatatca acaagatcag gggaccaaag agcggcttca ccgaggcctt tacacctcag
     1321 ttctctccaa agaatatcag ccctcaggac ctgacccaca acaatatcct gacactggag
     1381 gagtgctatg tgccccctaa cgtggagcac atcttctgta ggttttctct gcgcgtgcag
     1441 gccaatagcc tggtgcccag cggctgctcc gatcctgagg tgttttccct gctgaaggag
     1501 ctggccgaga ccttcaagga gtgcggagga tacaaggagc tggccgtgcg gtattgtaga
     1561 aacatcctga tcggcacatg gctgtggcgg aaccagaata ccggcaatac acagatcgag
     1621 atcaagacct ctaagggcag ctgttacctg atcgacaaca caagaaagct ggcctgggag
     1681 agcaagtggg cctccgacga tctgaaggtg ctggaggagc tgtccaacga gatcgagtct
     1741 gccctgaccg accctaacgt gttctggagc gccgatatca cagccaagat cgaggcctcc
     1801 ttctgccagg aaatctaccc atctcagatc ctgaacgaca aggtgaagca gggcgaggcc
     1861 tccaagcagt ttgtgaaggc caagtgtgcc gatggccggt atgccgtgtc cttcaattct
     1921 gtgaagatcg gcgccgccct gcagagcatc gacgattggt gggacgagga tgcctccaag
     1981 aggctgcgcg tgcacgagtt cggagcagac aaggagatcg gagtggcacg gagaccaccc
     2041 gatagcgagc agaactttta ctccatcttc aagaataccg agtggtatct gtccgccctg
     2101 aagaattgta tcacaaacaa gaatgagaag atcgatcccg ccatctacta tctgttttct
     2161 gtgctgatca agggcggcat gttccagaag aaggccgagg ccaagaaggc cggctccgga
     2221 aaaagaaccg ccgacggcag cgaattcgag tcacccaaga agaagaggaa agtcgggtcc
     2281 tccgagggaa ggggctctct gctgacctgc ggcgacgtgg aggagaaccc tggacccgga
     2341 atgggccacc tgaaggagct gctggagatc accgacacca cagagaggga tcgctccctg
     2401 cggagagcct tctctcccta caccgccatg atcgacatca caggctccga ggccgtggcc
     2461 ctgatcatcc tgctgaatct gacatatcgc aagaaccagg tggacgatct gctggacaag
     2521 aagctggcca agcaggccct gaagagcgag gatcacatca acaagtgcat caaggagatc
     2581 gcctggtttc acacccacaa tctgaagtac ccagatatcc gggtgtccaa gcagaacctg
     2641 gccgtggagc cccctacact gcactcttat gtgctgagct ccgccaatta ccccaaggcc
     2701 tatggctgga gccacaattc cgccaaggtg aactttgcca agctgttcgt gagctacttt
     2761 aagtggcaga accaggtgtc ctggctggcc caggtgctgg ccaccaatag cgacaactgg
     2821 aagtctgcct tcacaagcct gggcctgtcc gtgaaggcct tcaagagcct gtgcgtgacc
     2881 gtgaagaata gcctgcctga ggaggccatc ccagactccg tggataggta ttctcgccag
     2941 atcagaatgc cataccacga cggctatctg gccgtgaccc ccgtgatcag ccacgtggtg
     3001 cagtccaaga tccagcaggc cgccatcgat aagcgggcca gattctccaa cgtggagttt
     3061 acacgcccag cagccgtgtc tatgctggcc gccagcctgg gaggcgtgat caatgtgctg
     3121 aactacccac cctatatcag aagcaagtac cacggcctgt ctaatagcag ggccttcaag
     3181 ctgaacaatg gccagaccgt gtttaacgtg gaggccctgc tgaagcctga gctgatcaag
     3241 gccctggagg gcatcatctt ctccaacaat gccctggccc tgaagcagag gcgccagcag
     3301 aaggtgaaga atatcaagga gctgcgcaac acactgctgg agtggttctc tccagtgttt
     3361 gagtggcggc tggacgccat cgagaatggc tacgatctgg agcagctgga gtccgcctct
     3421 gagagactgg agtataagat cctgtccctg cccgacaatg agctgccttc tctgaccatc
     3481 ccactgttca gactgctgaa cgagatgctg ggcggcgtga gcatgacaca gaggtacgcc
     3541 tttcacccca agctgatgtc ccctctgaag gccgccctgc agtggctgct ggtgaatctg
     3601 accgaccaga agcacgtgct gatcgaggag gacgatgagc actacagata tctgcacctg
     3661 tccggcatca gggtgtttga tgcccaggcc ctgtctaacc catactgctc tggcatcccc
     3721 agcctgacag ccgtgtgggg aatgatccac tcttatcaga ggaagctgaa tgaggccctg
     3781 ggcaccaacg tgcggttcac cagcttcagc tggttcatca gaaactacag cgccgtggca
     3841 ggcaagaagc tgcccgagct gagcctgcag ggagcacagc agtccagact gaagaggcct
     3901 ggcatcatcg acggcaagta ctgtgacctg gtgttcgatc tgatcatcca catcgatggc
     3961 tatgaggacg atctgcaggc cgtggacagc aagcctgata tcctgaaggc ccacttccca
     4021 tccaattttg caggaggcgt gatgcaccag ccagagctga actctaatat caactggtgc
     4081 tgtctgtata gcaatgagaa ccagctgttt gagaagctgc ggagactgcc cctgtccggc
     4141 tgctgggtca tgcctaccga gcacaagatc caggacctgg atgagctgct gctgctgctg
     4201 aactctgaca gcaagctgtc cccttctatg atgggctaca tgctgctgac cgagccaatg
     4261 gcccgcgtgg gcagcctgga gcggctgcac tgttacgccg agcctgccat cggcgtggtg
     4321 aagtatgagg ccgccacatc tgtgaggctg aagggcatcg gcaattattt caacagcgcc
     4381 ttttggatgc tggatgccca ggagaagttc atgctgatga agaaggtggg aagcggcaag
     4441 cggactgctg atggcagtga atttgagtcc ccaaagaaga agagaaaggt gggctcgagc
     4501 gagggaaggg gctccctgct gacttgtggc gacgtggagg agaaccctgg ccccagcatg
     4561 ggccagagat actactttac cgtgcatttc ctgcctaaac aagccaatct ggccctgctg
     4621 acaggcagat gcatcagcat catgcacggc tttatcctga agcacaacat cgagggaatg
     4681 ggcgttacct tcccagcctg gtccgacagc agcatcggca acgaaattgc cttcgtgtac
     4741 accgacaagg aaatcctcaa cacactgaag gaccaggcct acttcgtgga tatgcaggat
     4801 tgcggcttct tcaaggtctc ccaagtgctg gctgtgcccg attcttgcga ggaagtgcgg
     4861 ttcatccgga accaggccgt ggccaaaatc ttcaccggag agagcagaag aaggctgaaa
     4921 agactgcaga agagagccct ggccagaggc gaagatttca accccaagaa gatcgaggcc
     4981 cctcgggaaa tcgatatctt ccacagagtg gccatgacca gcaagagctc tcaggaggac
     5041 tacatcctgc acatccagaa acaggacgtg gactgtcagg ctgagcctta ctttagcaat
     5101 tacggcctgg ccagcaacga gaagttcaag ggaacagtgc ctgacctgag ccccagcatc
     5161 gacagaaacg ggtctggaaa gcgcaccgcc gatggttccg agttcgaaag ccccaaaaaa
     5221 aagcgcaagg tcggtagctc cgagggaaga ggctccctgc tgacatgtgg cgacgtggaa
     5281 gaaaaccctg gacctggcaa acggacagcc gacggaagcg agttcgagtc accgaaaaag
     5341 aagcgaaaag ttggctccgg aatggccttc ctgttttctc ccaaggcccg ggcctttagc
     5401 gacgagtccc tggagtctta tctgctgaga gtggtgagcg agaatttctt tgatagctac
     5461 gagggcctgt ccctggccat cagggaggag ctgcacgagc tggactttga ggcacacgga
     5521 gcattcccag tggacctgaa gcggctgaac gtgtaccacg ccaagcacaa ctctcacttc
     5581 agaatgagag ccctgggact gctggagacc ctgctggacc tgccccggta tgagctgcag
     5641 aagctggccc tgctgaagag cgatatcaag tttaacagct ccgtggccct gtacaacaat
     5701 ggcgtggaca tccctctgag gttcatcagg caccacgcag aggaggcagt ggattctatc
     5761 ccagtgtgca gccagtgtct ggccgaggag gcctacatca agcagtcctg gcacatcaag
     5821 tgggtgaatg cctgcaccaa gcaccagtgt gccctgctgc acaactgccc cgagtgttac
     5881 gcccctatca actatatcga gaatgagtcc atcacacact gctcttgtgg ctttgagctg
     5941 agctgcgcct ctaccagccc cgtgaataca ctgtccatcg agcacctgaa caagctgctg
     6001 gacaagggcg agaggaacga ttccaatcct ctgtttaaca atatgaccct gacagagagg
     6061 ttcgccgccc tgctgtggta tcaggagcgg tactctcaga ccgacaactt ctgcctgaat
     6121 gatgccgtga actactttag caagtggcct gccgtgttca atacagagct ggacgagctg
     6181 tccaagaacg ccgagatgaa gctgatcgat ctgtttaaca agaccgagtt caagtttatc
     6241 ttcggcgacg ccatcctggc ctgtccaagc acacagaagc agtccgagtc tcacttcatc
     6301 tacagggccc tgctggacta tctggtgacc ctggtggagt ccaatccaaa gacaaagaag
     6361 cccaacgccg ccgatctgct ggtgtctgtg ctggaggcag ccaccctgct gggcacaagc
     6421 gtggagcagg tgtaccgcct gtatcagaat ggcatcctgc agaccgcctt tcggcacaag
     6481 atgaatcaga gaatcaaccc ttacaagggc gccttctttc tgaggcacgt gatcgagtat
     6541 aagacatcct tcggcaacga taaggcccgc atgtacctgt ctgcctggtg agcggccgct
     6601 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     6661 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6721 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     6781 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     6841 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     6901 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6961 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     7021 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     7081 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     7141 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     7201 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     7261 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     7321 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     7381 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     7441 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7501 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7561 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7621 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     7681 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     7741 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     7801 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     7861 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     7921 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     7981 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     8041 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     8101 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     8161 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     8221 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     8281 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     8341 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     8401 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     8461 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     8521 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     8581 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     8641 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     8701 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     8761 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8821 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     8881 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     8941 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     9001 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     9061 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     9121 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9181 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9241 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9721 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9961 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    10021 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    10081 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    10141 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    10201 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    10261 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    10321 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    10381 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    10441 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    10501 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    10561 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    10621 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    10681 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10741 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10801 gatctt
//
