LOCUS       sequence-393130-        3852 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-6-RcTAL-9
FEATURES             Location/Qualifiers
     source          1..3852
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1732..1750)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1818..1922
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2144..2935
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3106..3694
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3595..3614
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3848..3852,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcaa atgacgctcc aatcacagac tgctaaagac
      121 tgccttgcat tagatggcgc tttaacgctg gtgcaatgcg aggccatcgc tacacatagg
      181 tcccgaataa gcgtcactcc tgccctcaga gagagatgtg caagggctca cgcccgtctg
      241 gaacacgcca tcgccgagca aagacatata tacggcataa ccactgggtt tggcccgctt
      301 gcaaatcgac tgattggagc agatcaaggc gcagaactcc aacaaaacct tatttaccac
      361 cttgccacgg gggttgggcc aaaattgagt tgggctgagg caagggccct gatgttggcc
      421 aggctgaact caattctcca gggagcttca ggggcttccc cagagacaat cgatcgtatc
      481 gtagcagtac tcaacgctgg atttgccccc gaggttccgg ctcaaggaac tgtcggcgca
      541 agcggtgacc tgaccccgct cgcacacatg gtgctcgctc tgcagggccg aggtcgtatg
      601 attgacccaa gtggccgtgt tcaagaggcc ggagccgtca tggatcgtct gtgcggtggg
      661 cccctgaccc tggctgccag ggacggactg gcactggtaa acggaacttc cgctatgacg
      721 gccattgccg ccctcaccgg tgtagaagct gccagagcca tagatgctgc tttaaggcac
      781 tccgctgtac ttatggaagt acttagcgga cacgcagagg catggcatcc tgcctttgca
      841 gagcttcgtc ctcatccagg tcaattgaga gccacagagc gtcttgcaca agccttagat
      901 ggcgccggta gagtgtgtag gaccttaact gctgcaagac gtcttaccgc cgccgacctg
      961 aggcccgagg accatcccgc acaggatgcc tactcattga gagtagttcc gcaattagta
     1021 ggggcagtct gggacacact tgattggcat gacagggtag tcacgtgcga gttaaacagc
     1081 gtcacagata accctatatt tcctgagggc tgcgctgtgc ctgctctcca tggcgggaac
     1141 tttatgggtg tacatgtcgc actcgcttct gatgcactga acgctgcatt ggtaactctg
     1201 gctggtttag ttgagaggca gatcgcaaga ctgacagacg agaagttgaa taagggctta
     1261 ccagcattcc tccacggagg acaggctgga ttacaaagtg ggttcatggg ggcccaagtc
     1321 accgctactg ctttgctcgc cgaaatgcga gctaacgcta cccctgtctc agtacagtct
     1381 ctttcaacaa atggcgcaaa ccaggacgtt gtgtccatgg gtacgattgc agccaggcga
     1441 gcacgagcac aattgttacc gctgtcacag attcaggcta ttttggcatt agctttggca
     1501 caagctatgg atttgttaga cgatccggaa ggacaagcag gctggtcact cactgccagg
     1561 gacctcaggg accgaatcag ggctgtaagt cccggattac gtgccgatag gccacttgcc
     1621 gggcacattg aggcagtcgc acaaggactt cgtcatccat ctgccgctgc cgaccctccg
     1681 gctagtggtt gagctttgag accacgaagt ggctcttcag tggacgaaag ggcctcgtga
     1741 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1801 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1861 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1921 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     1981 tggcggtttt catggcttgt tatgactgtt tttttttggg gtacagtcta tgcctcgggc
     2041 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     2101 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat
     2161 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     2221 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     2281 cgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     2341 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     2401 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     2461 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     2521 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     2581 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     2641 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     2701 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     2761 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     2821 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     2881 ggaagaattt gtccattacg taaaaggcga gatcaccaag gtagtcggca aataactgtc
     2941 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3001 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3061 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3121 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3181 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3241 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3301 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3361 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3421 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3481 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3541 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3601 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3661 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3721 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3781 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     3841 cgagcgcagc ga
//
