LOCUS       sequence-393132-        3484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobrick (MoClo) Level 0 Part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pMC-1-SlEf1a-6
FEATURES             Location/Qualifiers
     source          1..3484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1364..1382)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1450..1554
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1776..2567
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2738..3326
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3227..3246
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3480..3484,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
       61 gccgattcat taatcactct gtggtctcag gagggtttct atttgtaagc taatcttttt
      121 cagttatcct tttttttaaa tctttattat tattatagct atatctttta tcttttaaaa
      181 ttaacattat ctattaaaga taatttcaat aaaagagtaa aaattaattt agagttctac
      241 tttcttcaaa tttctatttt aaaaaataat tttaaaactt gatgtatttt tttacgtggt
      301 ttttcactat gatttgattt ctgttttatt ataatatgta taaatataaa aatagatatt
      361 ccataacata ttataaaaaa tgtaaggggc atttacgtaa atagatagac ttaaaagagg
      421 caccgagtga accctaattc tcatcgttga gactataaaa tgcccattat cccattcgca
      481 gtctcttcat tacttttgct gtgatttctc ctcagctgtg ccgcatatcg cctaattttt
      541 cttctctaag gtttcgtcat cttcaccaat ttctttaatc tcgattctat tttttatgtt
      601 tgatctgtta ttgttctgtc actacatgtg tttttcagtt gttttactag atgattttca
      661 ctgacttctt gttagatcat acatatattg aaaatgtttt ggattgactt ttttgtattg
      721 tgaatatctg ttattgtttg attgttgttc agtatttaca cagccgatct gtgttatgag
      781 cttggtaata actatttctc tgtatgtaaa tacagatctg ttaatgtttg taatcaattt
      841 ttcatatgca ctgttgatat tgttctctct cctgtcctgt tatatgttgg tatgattcgg
      901 tttttgtata acttgaacta aacactagtc ctaaatgttt tttttactat ttaagattta
      961 tataatatgg atagattttt tgagttccta gtctctgaag aggttaagct tgctgtagtt
     1021 gtttaccagt tgagttgcaa tactaaaaat caattcaatt actgatattt tttgccgttt
     1081 aggtttttga caaagtactt taatttgctt tattgaacta aaaacgtagt cctgaattca
     1141 tggcaagtgt gaaagctata gttcactgtt tttgttgcaa ttcttgaaaa atcaattggt
     1201 caagctataa tggatttact ttttctgttt taaatgttga atgcgctgaa tttatgaatg
     1261 ggttgcatgg tttttgaaat atgttgttgt gtgttgtgta aatgcagttt cttagtgtct
     1321 caagaatgtg agaccacgaa gtggctcttc agtggacgaa agggcctcgt gatacgccta
     1381 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     1441 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1501 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgcgc
     1561 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     1621 ttcatggctt gttatgactg tttttttttg gggtacagtc tatgcctcgg gcatccaagc
     1681 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     1741 agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag
     1801 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     1861 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agcgatattg
     1921 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     1981 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     2041 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     2101 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     2161 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     2221 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     2281 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     2341 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     2401 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     2461 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     2521 ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag
     2581 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     2641 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2701 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2761 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2821 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2881 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2941 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3001 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3061 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3121 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3181 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3241 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3301 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3361 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3421 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3481 gcga
//
