LOCUS       sequence-393134-        2053 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 module 5 with BsaI expansion point.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pJMC-5-BsaI
FEATURES             Location/Qualifiers
     source          1..2053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        135..239
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1307..1895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1796..1815
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2049..2053,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc acctgctttt cctcaccgtg agaccaacaa
       61 caaggtctct agtgcggatt ttgcaggtgt gatgaaaagc cttagggtca ggtggcactt
      121 ttcggggaaa tgtgcgatga acccctattt gtttattttt ctaaatacat tcaaatatgt
      181 atccgctaat gagacaataa ccctgataaa tgcttcaatt atattgaaaa aggaagagta
      241 tgagtatcca acatttccgt gtcgccctta ttcccttttt tgcggccttc tgccttcctg
      301 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgccc
      361 gagtgggtta catttaactg gatctcaaca gcggcaaaat cttagagagt tttcgccccg
      421 aagaacgctt cccaatgatg agcactttca aagttctgct atgtggcgcg gtgttatccc
      481 gtattgatgc tgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
      541 ttgagtattc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
      601 gtagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgattg
      661 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
      721 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
      781 ctgtagcaat ggcaacaact ctccgcaaac tattaactgg cgaactactt actcttgctt
      841 cacgccagca actcattgac tggatggagg cggacaaagt tgcaggacca cttctgcgct
      901 cggcacttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggctctc
      961 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     1021 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     1081 cactgattaa gcattggtaa ctgtcagacg aagggaaata aatagtagcc cgcctgatgt
     1141 gcgggctttt ttttggtggt ggttaccagt gtcgcttagc taccgtaggt cctcttaggc
     1201 ggtccgatta gcgacatagt catcaatgcc ttgacggcaa aatcccttaa cgtgagtttt
     1261 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1321 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1381 tgccggatca agagctacta actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1441 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1501 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1561 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1621 gctgaacggg gggttcgtgc tcacagccca gcttggagcg aacgacctac accgaactga
     1681 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     1741 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     1801 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     1861 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     1921 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     1981 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2041 ccgagcgcag cga
//
