LOCUS       sequence-393136-        9363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of HSF1 with HSF2 DBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Price RM, Budzynski MA, Shen J, Mitchell JE, Kwan JZJ, Teves SS
  TITLE     Heat shock transcription factors demonstrate a distinct mode of 
            interaction with mitotic chromosomes.
  JOURNAL   Nucleic Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304.
  PUBMED    37114996
REFERENCE   2  (bases 1 to 9363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Apr 28:gkad304. doi: 10.1093/nar/gkad304."
COMMENT     Sequence Label: H2DBD-H1-Halo
FEATURES             Location/Qualifiers
     source          1..9363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(3..9,10..27,28..33))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    48..69
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(186..203)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(357..945)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(437..456)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1116..1907,1908..1976))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1739..1758
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1977..2081)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2107..2562)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2244..2265)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2456..2475
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2689..2711
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2703..2720
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2704..2720
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2850..2912
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        3365..3576
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3589..3857
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5443..5463
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             5473..6363
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             6364..6387
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6412..6984
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6579..6596)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6806..6825
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6993..7787
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7047..7066)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7657..7676
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7847..7867)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    7909..8030
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7946..7965)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8000..8019
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8793..8827
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9318..9334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9318..9334)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9331..9353)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9342..9358
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
       61 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      121 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      181 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      241 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      301 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      361 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      421 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      481 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      541 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      601 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      661 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      721 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      781 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      841 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      901 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      961 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1981 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2041 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2101 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2161 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2221 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2281 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2341 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     2401 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     2461 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     2521 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     2581 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     2641 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     2701 gttgtaaaac gacggccagt gagcgcgcct cgttcattca cgtttttgaa cccgtggagg
     2761 acgggcagac tcgcggtgca aatgtgtttt acagcgtgat ggagcagatg aagatgctcg
     2821 acacgctgca gaacacgcag ctagattaac cctagaaaga taatcatatt gtgacgtacg
     2881 ttaaagataa tcatgtgtaa aattgacgca tgtgttttat cggtctgtat atcgaggttt
     2941 atttattaat ttgaatagat attaagtttt attatattta cacttacata ctaataataa
     3001 attcaacaaa caatttattt atgtttattt atttattaaa aaaaacaaaa actcaaaatt
     3061 tcttctataa agtaacaaaa cttttatgag ggacagcccc cccccaaagc ccccagggat
     3121 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt
     3181 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa
     3241 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc
     3301 tggggggata cggggaaaag gcctccacgg ccaaggatct gcgatcgctc cggtgcccgt
     3361 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     3421 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     3481 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     3541 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     3601 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     3661 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     3721 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     3781 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     3841 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacta tgaagcagag ttccaacgtg
     3901 ccggctttcc tcagcaagct gtggacgctt gtggaggaaa cccacaccaa cgagttcatc
     3961 acctggagtc agaatggaca aagttttctg gtcttggatg agcaaagatt tgcaaaggaa
     4021 attcttccta agtacttcaa acacaataac atggcgagct ttgtgagaca actaaatatg
     4081 tatggcttcc gaaaagtagt gcatatcgaa tctggaatta tcaaacagga aagagatggc
     4141 cctgttgaat ttcagcatcc ttatttcaag caaggccagg atgacctgtt ggagaacatt
     4201 aaaaggaagg tttcatcttc aaaaccagag gaaaataaaa ttcgtcagga cagtgtcacc
     4261 cggctgttga cagatgtgca gctgatgaag gggaaacagg agtgtatgga ctccaagctc
     4321 ctggccatga agcacgagaa cgaggccctg tggcgggagg tggccagcct tcggcagaag
     4381 catgcccagc agcaaaaagt tgtcaacaag ctcattcagt tcctgatctc actggtgcag
     4441 tcgaaccgga tcctgggggt gaagagaaag atccctctga tgttgagtga cagcaactca
     4501 gcacactctg tgcccaagta tggtcgacag tactccctgg agcatgtcca tggtcctggc
     4561 ccatactcag ctccatctcc agcctacagc agctctagcc tttactcctc tgatgctgtc
     4621 accagctctg gacccataat ctccgatatc actgagctgg ctcccaccag ccctttggcc
     4681 tccccaggca ggagcataga tgagaggcct ctgtccagca gcactctggt ccgtgtcaag
     4741 caagagcccc ccagcccacc tcacagccct cgggtactgg aggcgagccc tgggcgccca
     4801 tcctccatgg ataccccttt gtccccaact gccttcattg actccatcct tcgagagagc
     4861 gagcctaccc ctgctgcctc aaacacagcc cctatggaca caaccggagc ccaagccccc
     4921 gcactcccga ccccctccac ccctgagaag tgcctcagcg tagcctgcct agacaagaac
     4981 gagctaagtg atcacctgga tgccatggac tccaacctgg acaacctgca gaccatgctg
     5041 acaagccacg gcttcagtgt ggacaccagt gccctgctgg acctgttcag cccctcggtg
     5101 accatgcccg acatgagcct gcctgacctg gacagcagcc tggccagcat tcaggagctt
     5161 ctgtctccac aagagcctcc caggcctatt gaggcagaga acagtaaccc cgactcagga
     5221 aagcagctgg tgcactacac ggctcagcct ctgttcctgc tggatcctga tgctgtggac
     5281 acagggagca gtgagctgcc tgtgctcttt gagctggggg agagctccta cttctctgag
     5341 ggggatgact acacggatga tcccaccatc tctcttctga caggcactga accccataaa
     5401 gccaaggacc ccactgtctc ctcctcgagg actagtcctc gagaggatct gtactttcag
     5461 agcggaggct ccatggcaga aatcggtact ggctttccat tcgaccccca ttatgtggaa
     5521 gtcctgggcg agcgcatgca ctacgtcgat gttggtccgc gcgatggcac ccctgtgctg
     5581 ttcctgcacg gtaacccgac ctcctcctac gtgtggcgca acatcatccc gcatgttgca
     5641 ccgacccatc gctgcattgc tccagacctg atcggtatgg gcaaatccga caaaccagac
     5701 ctgggttatt tcttcgacga ccacgtccgc ttcatggatg ccttcatcga agccctgggt
     5761 ctggaagagg tcgtcctggt cattcacgac tggggctccg ctctgggttt ccactgggcc
     5821 aagcgcaatc cagagcgcgt caaaggtatt gcatttatgg agttcatccg ccctatcccg
     5881 acctgggacg aatggccaga atttgcccgc gagaccttcc aggccttccg caccaccgac
     5941 gtcggccgca agctgatcat cgatcagaac gtttttatcg agggtacgct gccgatgggt
     6001 gtcgtccgcc cgctgactga agtcgagatg gaccattacc gcgagccgtt cctgaatcct
     6061 gttgaccgcg agccactgtg gcgcttccca aacgagctgc caatcgccgg tgagccagcg
     6121 aacatcgtcg cgctggtcga agaatacatg gactggctgc accagtcccc tgtcccgaag
     6181 ctgctgttct ggggcacccc aggcgttctg atcccaccgg ccgaagccgc tcgcctggcc
     6241 aaaagcctgc ctaactgcaa ggctgtggac atcggcccgg gtctgaatct gctgcaagaa
     6301 gacaacccgg acctgatcgg cagcgagatc gcgcgctggc tgtcgacgct cgagatttcc
     6361 ggcgactaca aagacgatga cgacaagcgt acgtaaggat ccgcggccgc gcccctctcc
     6421 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     6481 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     6541 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     6601 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     6661 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     6721 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     6781 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     6841 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     6901 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     6961 tttcctttga aaaacacgat aatacctccg gaatgattga acaagatgga ttgcacgcag
     7021 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     7081 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     7141 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     7201 tggccgcgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     7261 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     7321 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     7381 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     7441 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     7501 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     7561 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     7621 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     7681 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     7741 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagtc gacaatcaac
     7801 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7861 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc gttaactaaa cttgtttatt
     7921 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7981 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8041 aattgactca aatgatgtca attagtctat cagaagctca tctggtctcc cttccggggg
     8101 acaagacatc cctgtttaat atttaaacag cagtgttccc aaactgggtt cttatatccc
     8161 ttgctctggt caaccaggtt gcagggtttc ctgtcctcac aggaacgaag tccctaaaga
     8221 aacagtggca gccaggttta gccccggaat tgactggatt ccttttttag ggcccattgg
     8281 tatggctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt
     8341 tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg
     8401 gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc
     8461 ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctga
     8521 tatctataac aagaaaatat atatataata agttatcacg taagtagaac atgaaataac
     8581 aatataatta tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt
     8641 ttgactcacg cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc
     8701 ctcacgggag ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct
     8761 atttagaaag agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt
     8821 tctagggtta atctagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca
     8881 tcgcccaagc tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga
     8941 ggttgaagcg gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg
     9001 aaaacgcacg tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga
     9061 actgttcgtt caggccacct gggataccag ttcgtcgcgg cttttccgga cacagttccg
     9121 gatggtcagc ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg
     9181 tgccggtgtg cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg
     9241 gtatcctggc tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg
     9301 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     9361 cca
//
