LOCUS       sequence-393138-        3472 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 1 Module 6 destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Chamness JC, Kumar J, Cruz AJ, Rhuby E, Holum MJ, Cody JP, Tibebu R,
            Gamo ME, Starker CG, Zhang F, Voytas DF
  TITLE     An extensible vector toolkit and parts library for advanced 
            engineering of plant genomes.
  JOURNAL   Plant Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312.
  PUBMED    36896468
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Genome. 2023 Mar 9:e20312. doi: 10.1002/tpg2.20312."
COMMENT     Sequence Label: pJMC-6
FEATURES             Location/Qualifiers
     source          1..3472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(86..91,92..109,110..116)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             170..850
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             1170..1475
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1354..1373
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     promoter        1553..1657
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2726..3314
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3215..3234
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3468..3472,1..13)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcagtgagc gaggaagcgg aagagcgccc acctgctttt cggaggcgcg ccggagtgag
       61 accggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
      121 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa
      181 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
      241 atttcagtca gtcgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
      301 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
      361 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat
      421 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
      481 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
      541 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
      601 cgtttcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
      661 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
      721 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
      781 gcttaatgaa ttacaacagt actgcgatga gtggcaggcg gggcgtaatc tagaggatcc
      841 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
      901 atatatactg atatgtatac ccgaagtatg tcaaaaggag gtatgctatg aagcagcgta
      961 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     1021 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     1081 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     1141 ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag
     1201 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac
     1261 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     1321 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     1381 tgccggtttc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     1441 aaaacgccat taacctgatg ttctggggaa tataaggtct ctcgctgagc tcctgctttt
     1501 gcaggtgtga tgaaaagcct tagggtcagg tggcactttt cggggaaatg tgcgatgaac
     1561 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctaatga gacaataacc
     1621 ctgataaatg cttcaattat attgaaaaag gaagagtatg agtatccaac atttccgtgt
     1681 cgcccttatt cccttttttt gcggccttct gccttcctgt ttttgctcac ccagaaacgc
     1741 tggtgaaagt aaaagatgct gaagatcagt tgggtgcccg agtgggttac attgaactgg
     1801 atctcaacag cggcaaaatc ttagagagtt ttcgccccga agaacgcttc ccaatgatga
     1861 gcactttcaa agttctgcta tgtggcgcgg tgttatcccg tattgatgct gggcaagagc
     1921 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtattca ccagtcacag
     1981 aaaagcatct tacggatggc atgacagtaa gagaattatg tagtgctgcc ataaccatga
     2041 gtgataacac tgcggccaac ttacttctga caacgattgg aggaccgaag gagctaaccg
     2101 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     2161 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaactc
     2221 tccgcaaact attaactggc gaactactta ctcttgcttc acgccagcaa ctcattgact
     2281 ggatggaggc ggacaaagtt gcaggaccac ttctgcgctc ggcacttccg gctggctggt
     2341 ttattgctga taaatctgga gccggtgagc gtggctctcg cggtatcatt gcagcactgg
     2401 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2461 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2521 tgtcagacga agggaaataa atagtagccc gcctgatgtg cgggcttttt tttggtggtg
     2581 gttaccagtg tcgcttagct accgtaggtc ctcttaggcg gtccgattag cgacatagtc
     2641 atcaatgcct tgacggcaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2701 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2761 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctactaa
     2821 ctctttttcc gaaggtaact ggcttcagta gagcgcagat accaaatact gttcttctag
     2881 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2941 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3001 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgct
     3061 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3121 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3181 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3241 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3301 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3361 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3421 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc ga
//
