LOCUS       sequence-393139-       13855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries a Sleeping Beauty (SB) transposon vector that can be used to
            deliver an activated human CTNNB1(S33Y) transgene into cells, when a
            source of SB transposase is also co-introduced..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13855)
  AUTHORS   Keng VW, Sia D, Sarver AL, Tschida BR, Fan D, Alsinet C, Sole M, Lee
            WL, Kuka TP, Moriarity BS, Villanueva A, Dupuy AJ, Riordan JD, Bell 
            JB, Silverstein KA, Llovet JM, Largaespada DA
  TITLE     Sex bias occurrence of hepatocellular carcinoma in Poly7 molecular 
            subclass is associated with EGFR.
  JOURNAL   Hepatology. 2013 Jan;57(1):120-30. doi: 10.1002/hep.26004. Epub 2012
            Oct 19.
  PUBMED    22899566
REFERENCE   2  (bases 1 to 13855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/hep.26004";
            journalName: "Hepatology"; date: "2013-01"; volume: "57"; issue: 
            "1"; pages: "120-30"
COMMENT     Sequence Label: pT2/GD-IRES-GFP-CTNNB1
FEATURES             Location/Qualifiers
     source          1..13855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             40..81
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             91..108
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(248..821,822..824,825..833)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             834..2486
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(900..918)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2653..2708
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3409..3429)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3409..3427)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3448..3464)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3448..3464)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3461..3483)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3472..3488
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3496..3502,3503..3520,3521..3526))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3541..3562
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3679..3696)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3850..4438)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3930..3949)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4609..5400,5401..5469))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAAKLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5232..5251
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5470..5574)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5601..6056
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5688..5707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5898..5919
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6182..6204
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6196..6213
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6197..6213
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6223..6241
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    6908..6963
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(join(7134..7844,7845..7847,7848..7850))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7134..7844,7845..7847,7848..7850))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7176..7197)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7525..7544
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7784..7805
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(7851..7859,7860..7862,7863..8437)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     promoter        complement(9830..10329)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9878..9897)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     enhancer        10437..10740
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10742..11017
                     /label=chicken beta-actin promoter
     primer_bind     11273..11292
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        11363..11381
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    11411..11435
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11459..13801
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDYGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     CDS             11459..13801
                     /codon_start=1
                     /gene="Homo sapiens CTNNB1"
                     /product="human cadherin-associated protein that also plays
                     a role in the canonical Wnt signaling pathway"
                     /label=beta-catenin
                     /note="also known as catenin beta-1"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDYGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     CDS             13802..13825
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(join(13845..13855,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggttgatct agagggcccg cggttcgaag gtaagcctat ccctaaccct
       61 ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg agtttggccg
      121 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
      181 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattccgcc cccccccccc
      241 cccccccccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
      301 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
      361 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
      421 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
      481 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
      541 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
      601 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
      661 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
      721 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa
      781 ccacggggac gtggttttcc tttgaaaaac acgattgcta tatggccaca accatggaag
      841 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg
      901 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta
      961 cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt
     1021 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg
     1081 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg
     1141 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc
     1201 ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc
     1261 tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt
     1321 cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtgc
     1381 cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg
     1441 gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca
     1501 gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat
     1561 tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg
     1621 tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc
     1681 aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca
     1741 aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag
     1801 tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca
     1861 ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg
     1921 gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg
     1981 gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa
     2041 acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca
     2101 tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta
     2161 agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca
     2221 acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg
     2281 ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg
     2341 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac
     2401 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca
     2461 agaagggcgg aaagatcgcc gtgtaattct agagcggccg aacgcgtctt ccggagtgta
     2521 caggcatgcg gtcgacccac tagtgagtcg tattacccaa tgccctggct cacaaatacc
     2581 actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     2641 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     2701 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     2761 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     2821 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     2881 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     2941 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     3001 cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcatacc ggtcaagcta
     3061 ggctactcga aatgtttgac ccaagttaaa caatttaaag gcaatgctac caaatactaa
     3121 ttgagtgtat gtaaacttct gacccactgg gaatgtgatg aaagaaataa aagctgaaat
     3181 gaatcattct ctctactatt attctgatat ttcacattct taaaataaag tggtgatcct
     3241 aactgaccta agacagggaa tttttactag gattaaatgt caggaattgt gaaaaagtga
     3301 gtttaaatgt atttggctaa ggtgtatgta aacttccgac ttcaactgta tagggatcct
     3361 ctagctagag tcgacctcga gggggggccc ggtacccagc ttttgttccc tttagtgagg
     3421 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     3481 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     3541 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3601 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3661 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3721 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3781 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4021 ttcgggaacc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4261 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     4321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     4381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     4441 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     4501 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     4561 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4621 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4681 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4741 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4801 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4861 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4921 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4981 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5041 cggtcctccg atcgttgtca gaagtaactt ggccgcagtg ttatcactca tggttatggc
     5101 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5161 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5221 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5281 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     5341 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     5401 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     5461 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     5521 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     5581 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggagtggt
     5641 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     5701 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     5761 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     5821 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     5881 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5941 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     6001 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttccat
     6061 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     6121 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     6181 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     6241 ggcgaattgg agctcggatc cctatacagt tgaagtcgga agtttacata cacttaagtt
     6301 ggagtcatta aaactcgttt ttcaactact ccacaaattt cttgttaaca aacaatagtt
     6361 ttggcaagtc agttaggaca tctactttgt gcatgacaca agtcattttt ccaacaattg
     6421 tttacagaca gattatttca cttataattc actgtatcac aattccagtg ggtcagaagt
     6481 ttacatacac taagttgact gtgcctttaa acagcttgga aaattccaga aaatgatgtc
     6541 atggctttag aagctagatc agcttgggct gcaggtcgag ggatctccat aagagaagag
     6601 ggacagctat gactgggagt agtcaggaga ggaggaaaaa tctggctagt aaaacatgta
     6661 aggaaaattt tagggatgtt aaagaaaaaa ataacacaaa acaaaatata aaaaaaatct
     6721 aacctcaagt caaggctttt ctatggaata aggaatggac agcagggggc tgtttcatat
     6781 actgatgacc tctttatagc caacctttgt tcatggcagc cagcatatgg gcatatgttg
     6841 ccaaactcta aaccaaatac tcattctgat gttttaaatg atttgccctc ccatatgtcc
     6901 ttccgagtga gagacacaaa aaattccaac acactattgc aatgaaaata aatttccttt
     6961 attagccaga agtcagatgc tcaaggggct tcatgatgtc cccataattt ttggcagagg
     7021 gaaaaagatc tcagtggtat ttgtgagcca gggcattggc cacaccagcc accaccttct
     7081 gataggcagc ctgcacctga tgaggagtga attgatctgg ccatctagag cggcttgtac
     7141 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
     7201 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
     7261 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
     7321 acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc
     7381 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
     7441 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
     7501 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
     7561 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
     7621 tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag
     7681 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
     7741 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
     7801 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggttgtggcc
     7861 atattatcat cgtgtttttc aaaggaaaac cacgtccccg tggttcgggg ggcctagacg
     7921 tttttttaac ctcgactaaa cacatgtaaa gcatgtgtac cgaggcccca gatcagatcc
     7981 catacaatgg ggtaccttct gggcatcctt cagccccttg ttgaatacgc ttgaggagag
     8041 ccatttgact ctttccacaa ctatccaact cacaacgtgg cactggggtt gtgccgcctt
     8101 tgcaggtgta tcttatacac gtggcttttg gccgcagagg cacctgtcgc caggtggggg
     8161 gttccgctgc ctgcaaaggg tcgctacaga cgttgtttgt cttcaagaag cttccagagg
     8221 aactgcttcc ttcacgacat tcaacagacc ttgcattcct ttggcgagag gggaaagacc
     8281 cctaggaatg ctcgtcaaga agacagggcc aggtttccgg gccctcacat tgccaaaaga
     8341 cggcaatatg gtggaaaata acatatagac aaacgcacac cggccttatt ccaagcggct
     8401 tcggccagta acgttagggg ggggggaggg agaggggcgg atctcgagca tgcgatatcg
     8461 ctagggccgc tcaggctggt gaaagggcag gcagcacttt cccagcacac tggccaaagc
     8521 caacacggta gccgtccccc tggcagtgac ctgttatgat gacttcatcg ccgtccagca
     8581 ggaaggtcct ggtctgcccc tgctccacat cgatggcctt tgttcccttc caggacagtt
     8641 ccagcatgga gccaaagctt tcagggtctg atccactgat ggttccagaa gccaagaggt
     8701 ccccaggtct caggttgcat ccattaacag agtggtgtgt gagttgctgc agcatggtcc
     8761 agtacatgtg cttaaagtta gacctgcaga tggtagccgc ctggctcatt ccttctcctt
     8821 tcaaagagac agacaggttg atatcaaatg tgtagggctg gctgtggcag agatatggca
     8881 agggcttggg gtcctgcttt gggtttggca ccacaaaggg catgagggca tccataggca
     8941 ccacccacgg ggagattgtg gttccaaagc ttttccccag gaatggccca agtgggacgt
     9001 actcccattg ctggatgtct cgtgcgctcc agtcgttcat gaggaccatc ccgaaaatgt
     9061 gttcatgggc tttggaaatg gggattggct ctccgaatct gttcccaggg cctacgaaga
     9121 aagccatttc caactccatg tctaagagtc tgcaggcacc atacacagga ggctttgagt
     9181 tatcaggtct catctgcccc atgggtcttc gaatcggggt tccagatacc acaatggagg
     9241 aagctcggcc atggtatccc acaggtaagt ggagccaatt tggcaacagc gcattctcct
     9301 tgcctctgaa cataatgcca acattggtgg catgctgccg agaagagtag aagtccgtgt
     9361 agtctcctat ggtagcagga aggtgcattg tcgcagaagc ctgggaggtg aatgcacgct
     9421 gccgaagctc cttgtcatct ctgagccggg cttggctggc agacagtaag ttctgtaagg
     9481 atgctcttgc ctccttccat gcagcttgac ccagacccat gaagttattg agagttgtct
     9541 catcgaagac atgttgatgt ttggaaaggg caggtccggt aaagaggtgt ttaatgacac
     9601 tcaggtccaa gatctggtca ccgatggcta caccaatccg tggctttggg ttgctttgag
     9661 tggagaaaac accatagggc aggttttgga tgggaaagtc ggagtcctcg gccactggaa
     9721 taaagctcat ggtgaattag gagtgaattg atctggccat ctagagcggc cgcgcgcact
     9781 agtgaattcc atggataatt cgattctcga gatctagatg gatgcaggtc gaaaggcccg
     9841 gagatgagga agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg
     9901 ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg agcccgcccc
     9961 cggacccacc ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc tgctattggc
    10021 cgctgcccca aaggcctacc cgcttccatt gctcagcggt gctgtccatc tgcacgagac
    10081 tagtgagacg tgctacttcc atttgtcacg tcctgcacga cgcgagctgc ggggcggggg
    10141 ggaacttcct gactagggga ggagtagaag gtggcgcgaa ggggccacca aagaacggag
    10201 ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc
    10261 gccaagtgcc cagcggggcc gctaaagcgc atgctccaga ctgccttggg aaaagcgcct
    10321 cccctacccg gtagggcgcg aaacctagta atcactagtg aattatcaag caaacctagt
    10381 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
    10441 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
    10501 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
    10561 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
    10621 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
    10681 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
    10741 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
    10801 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
    10861 gsgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc
    10921 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
    10981 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg
    11041 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
    11101 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
    11161 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggagct
    11221 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
    11281 ctggttattg tgctgtctca tcattttggc aaagaattct ctggctaact agagaaccca
    11341 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
    11401 taagctatca acaagtttgt acaaaaaagc aggcaatgag gaatccccag cgtggacaat
    11461 ggctactcaa gctgatttga tggagttgga catggccatg gaaccagaca gaaaagcggc
    11521 tgttagtcac tggcagcaac agtcttacct ggactatgga atccattctg gtgccactac
    11581 cacagctcct tctctgagtg gtaaaggcaa tcctgaggaa gaggatgtgg atacctccca
    11641 agtcctgtat gagtgggaac agggattttc tcagtccttc actcaagaac aagtagctga
    11701 tattgatgga cagtatgcaa tgactcgagc tcagagggta cgagctgcta tgttccctga
    11761 gacattagat gagggcatgc agatcccatc tacacagttt gatgctgctc atcccactaa
    11821 tgtccagcgt ttggctgaac catcacagat gctgaaacat gcagttgtaa acttgattaa
    11881 ctatcaagat gatgcagaac ttgccacacg tgcaatccct gaactgacaa aactgctaaa
    11941 tgacgaggac caggtggtgg ttaataaggc tgcagttatg gtccatcagc tttctaaaaa
    12001 ggaagcttcc agacacgcta tcatgcgttc tcctcagatg gtgtctgcta ttgtacgtac
    12061 catgcagaat acaaatgatg tagaaacagc tcgttgtacc gctgggacct tgcataacct
    12121 ttcccatcat cgtgagggct tactggccat ctttaagtct ggaggcattc ctgccctggt
    12181 gaaaatgctt ggttcaccag tggattctgt gttgttttat gccattacaa ctctccacaa
    12241 ccttttatta catcaagaag gagctaaaat ggcagtgcgt ttagctggtg ggctgcagaa
    12301 aatggttgcc ttgctcaaca aaacaaatgt taaattcttg gctattacga cagactgcct
    12361 tcaaatttta gcttatggca accaagaaag caagctcatc atactggcta gtggtggacc
    12421 ccaagcttta gtaaatataa tgaggaccta tacttacgaa aaactactgt ggaccacaag
    12481 cagagtgctg aaggtgctat ctgtctgctc tagtaataag ccggctattg tagaagctgg
    12541 tggaatgcaa gctttaggac ttcacctgac agatccaagt caacgtcttg ttcagaactg
    12601 tctttggact ctcaggaatc tttcagatgc tgcaactaaa caggaaggga tggaaggtct
    12661 ccttgggact cttgttcagc ttctgggttc agatgatata aatgtggtca cctgtgcagc
    12721 tggaattctt tctaacctca cttgcaataa ttataagaac aagatgatgg tctgccaagt
    12781 gggtggtata gaggctcttg tgcgtactgt ccttcgggct ggtgacaggg aagacatcac
    12841 tgagcctgcc atctgtgctc ttcgtcatct gaccagccga caccaagaag cagagatggc
    12901 ccagaatgca gttcgccttc actatggact accagttgtg gttaagctct tacacccacc
    12961 atcccactgg cctctgataa aggctactgt tggattgatt cgaaatcttg ccctttgtcc
    13021 cgcaaatcat gcacctttgc gtgagcaggg tgccattcca cgactagttc agttgcttgt
    13081 tcgtgcacat caggataccc agcgccgtac gtccatgggt gggacacagc agcaatttgt
    13141 ggagggggtc cgcatggaag aaatagttga aggttgtacc ggagcccttc acatcctagc
    13201 tcgggatgtt cacaaccgaa ttgttatcag aggactaaat accattccat tgtttgtgca
    13261 gctgctttat tctcccattg aaaacatcca aagagtagct gcaggggtcc tctgtgaact
    13321 tgctcaggac aaggaagctg cagaagctat tgaagctgag ggagccacag ctcctctgac
    13381 agagttactt cactctagga atgaaggtgt ggcgacatat gcagctgctg ttttgttccg
    13441 aatgtctgag gacaagccac aagattacaa gaaacggctt tcagttgagc tgaccagctc
    13501 tctcttcaga acagagccaa tggcttggaa tgagactgct gatcttggac ttgatattgg
    13561 tgcccaggga gaaccccttg gatatcgcca ggatgatcct agctatcgtt cttttcactc
    13621 tggtggatat ggccaggatg ccttgggtat ggaccccatg atggaacatg agatgggtgg
    13681 ccaccaccct ggtgctgact atccagttga tgggctgcca gatctggggc atgcccagga
    13741 cctcatggat gggctgcctc caggtgacag caatcagctg gcctggtttg atactgacct
    13801 ggactacaag gacgacgatg acaagtaagc ggccccagtt ctagacccag ctttc
//
